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Based on the orthogonal experiment, the best fermentation condition of the strain was preliminarily found to be: Tryptone 1.33%, starch 1.33%, yeast 0.3%, at 25℃.

在此基础上进行正交实验分析得到了该菌株的最佳发酵条件为:蛋白胨和淀粉含量各为1.33%,酵母膏含量为0.3%,温度为25℃。

The optimal chitinase fermentation conditions of strain SWCH-6 were conformed by single-factor experiments and orthogonal experiments, they were colloidal chitin 25.0 g/L, tryptone 10.0 g/L, seawater 1 L, pH 8.5, 32℃, 150 r/min for 72 h.

采用单因素优化方法结合正交实验,得到菌株SWCH-6产几丁质酶的最佳发酵条件:胶体几丁质25.0 g/L,胰蛋白胨10.0 g/L,陈海水1.0 L, pH 8.5, 32℃, 150 r/min培养72 h;在该条件下酶活力达0.39 U/mL。

Antioxidant and antitumor activity of nine compounds from fermental mycelium of Xylaria nigripes were studied.

对从黑柄炭角菌发酵菌丝中分离得到的9个化合物进行抗氧化和抗肿瘤活性研究。

Rapeseed meal detoxicated with multi-microbes including SPT-3, rhizopus oligosprus and lactics etc by ferment in an appropriate fermentation condition and the application of the detoxicated rapeseed meal were studied.

研究了复合菌体系酶法菜籽粕脱毒及其应用,采用少孢子根霉菌、乳酸菌等菌种,在适宜的发酵条件下进行脱毒。

The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料,经过过滤去除菌体,上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸,聚唾液酸沉淀用去离子水溶解,再用过滤去除变性蛋白质,滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后,再用过量乙醇沉淀出聚唾液酸,聚唾液酸沉淀用超纯水溶解,用层析柱进行分级纯化,收集洗脱液经过透析,冷冻干燥得聚唾液酸产品。

The steps were as follows: Acquiring superant from the culture medium by centrifugation, discarding waste residue by filtration, concentrating by hollow-fiber membrane, treating with salting-out, dialyzing the product of salting-out, treating the dialysate by vaccum freeze dryer, dissolving solid , using the method of gel filtration chromatography with Sephadex G-100/G-75, and proof-test by filter-paper chromatography ,then preparation by thin-layer chromatography was carried out.

建立了分离纯化该物质的有效途径:发酵液离心除菌→抽滤除杂→中空纤维过滤浓缩→盐析→透析→真空冷冻干燥→溶解→交联葡聚糖凝胶Sephadex G-100凝胶分离→交联葡聚糖凝胶Sephadex G-75凝胶分离→纸层析检验→薄层层析制备。

Various grains and inocula were investigated for carotenoid production by solid-state fermentation using Penicillium sp.

研究了 7种谷物培养基及相关因素对青霉 PT95菌株进行类胡萝卜素固态发酵的影响作用。

In order to improve the quality and yield of Xiaoqu liquor,the key technical points were progressed discussing in depth in the product such as the grain′s marinating,braizing,culture saccharification,fermentation etc.

摘 要:为提高小曲白酒的质量和产量,将白酒生产技术中几个关键问题如泡粮、蒸粮、培菌、发酵、黄水分析等进行了探讨。

After 48 hours, the activity of the saccharifying typ...

并对工程菌株TI40(pBX96)的发酵条件做了初步摸索。

The fermrntation supernatant of endophytic actinomycetes GKSHJA and PR1-8 played a role in cucumber powdery mildew protection.

内生放线菌GKSHJA及PR1-8发酵上清液对黄瓜白粉病防治有较好的保护性作用。

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