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It was found that the positive mutants were obtained within the range of lower doses of 1-5 × 104 Roentgen in shake flask fermentations.

其中产量最高的两株是78-5-49和78-5-52,它们的发酵单位比原始菌株分别提高33.3和28.3%,且这两株菌株都是在10000μg/ml卡那霉素平板中找到的。

Thermoanaerobacter ethanolicus is a Gram-positive,anaerobic thermophile that can produce considerable amounts of ethanol from a wide range of polymeric and soluble carbohydrates.

嗜热厌氧乙醇菌是一种专性厌氧、嗜高温的革兰氏阳性菌,能广泛地以廉价的淀粉、木聚糖等已糖和戊糖为底物进行发酵,产生以乙醇为主的代谢产物。

SR-B, a colorless crystal antimicrobic compound, was extracted and purified from broth of marine actinomycete N331 isolated and screened from sea mud. Its molecular structure was characterized by MS and various NMR spectra.

从分离筛选的海洋放线菌N331的发酵液中分离、纯化得到一种无色结晶抗菌化合物SR-B,通过对其质谱、多种核磁共振谱的结构信息进行分析,鉴定其分子结构。

The characterization of the fermental Toxigenic Pasteurella multocida (Pm 13-1) is studied according to the vaccine standard.

为检验该培养方法培养出的T+Pm是否符合对T+Pm制苗用菌液的生物学特性鉴定标准,分别取样作培养特性检验、菌体形态检验、糖发酵实验、T+Pm毒素产生的检测及荚膜型鉴定。

The bacterial strain CCTCC No:M208015 provided by the invention is the bacterial strain which is firstly discovered in the phomopsis and can produce gallic acid through fermentation method, the product of the phomopsis bacteria is gallic acid which is the raw material of various medicinal substances.

本发明的菌株CCTCC No:M208015是第一次在拟茎点霉属中发现的能通过发酵法产没食子酸的菌株,其产物为现今多种药物原材料――没食子酸。

Their fermentation filtrates had significant inhibitory effects upon the pathogen, with the inhibitory rates as 73.72% and 75.32%, respectively.

sp。 Mali具有较强拮抗作用的菌株,其中菌株B86和B91的发酵滤液在活体上时苹果斑点落叶病的防治效果显著,分别为73.72%和75.32%。

And all the soil samples were collected from the surroundings of cinnamon oil factory, flower fertilizer and so on. On the whole, this research mainly included two aspects:(1) To screen and ferment the strains producing the aimed enzyme and study on the enzymatic synthesis reaction of natural benzaldehyde.

其研究内容主要包括两个方面:(1)产酶菌种的筛选、发酵以及酶催化合成天然苯甲醛反应的研究通过大量实验,采用常规方法培养时,共筛出1 81株对肉桂醛有耐受性的菌株;将常规培养基换成细菌培养基以后,共筛出36株菌。

31DH suspend in the dry red wine and ferment,the density of the dry immobilized 31DH was changed by various materials.

通过选用不同的材料调节干固菌胶粒的比重,使固定化酒类酒球菌31DH悬浮于干红葡萄酒中进行苹果酸—乳酸发酵,并测定这些材料对固定化胶粒相对扩散系数的影响。

Two outstanding fusants SR-7 and SR-T19 were chose out in the fusion group 90-11×FR-008, the former produces not only antibiotics the parents produced but also a new antibiotic effective gainst a wide range of both gram positive and negative bacteria, which the parents could not produce, and its product of the 37℃ fermentation was very effective against the cotton seedling damping off caused by Rhizoctonia sonali while product of the 26℃ was effective agaist the bacterial rots of turnip and Chinese cabbage; the latter have a much stronger activity against a wide range of fungi and yeasts than the parents.

在90-11×FR-008融合组合中,筛选获得2个优良融合子SR-7和SR-T19,前者除产生亲本产生的抗生素外,还产生亲本不产生的一种新的具有广谱抗细菌活性的碱性水溶性抗生素,防病试验表明,其37℃发酵产物对棉花苗期立枯病具有良好的防治效果,28℃发酵产物对萝卜和大白菜具有明显的防腐作用;后者与亲本FR-008的抗菌谱完全一致,但其活性强度都超过了FR-008;采用RAPD分子标记技术检测了SR-7和SR-T19与亲本的同源性及彼此间的差异,从而首次直接从DNA分子水平上验证了链霉菌的原生质体融合子及其遗传重组的多样性。

Methods The influence of inducer concentration and medium on the bacterial density and expression level of target protein in Erlenmeyer flask, as well as the influence of time for induction, method for fed batch and dissolved oxygen on those in 10 L fermentor, were explored.

在三角烧瓶中,探讨不同的诱导剂浓度和培养基对菌体密度和目的蛋白表达量的影响;在10L发酵罐中,探讨诱导时间、补料方式、溶氧量对菌体密度和目的蛋白表达量的影响。

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