发酵菌的

The research on the conditions of chlamydospore production for the liquid fermentation showed that, the better single factors were oat liquid media, 30℃, 120r/min surge culture, sucrose as carbon source,(NH_4)_2SO_4 as nitrogen source and the ratio of 20 to 1 for C/N. Under the better single factors mentioned above, the largest quantity of chlamydospore, i.e.3.05×10~7spores/ml, was obtained on the 10th day after culture.

木霉T-33菌株产生厚垣孢子的发酵条件研究表明，采用燕麦粉培养液、30℃、120r/min振荡培养、250ml三角瓶装入80ml培养液、接种5片活化2d的5mm直径的菌片，碳氮源选择蔗糖和硫酸铵，C/N为20/l，足适合T-33菌株产孢的最佳单因素条件(碳氮源实验基于Czapek培养基)；用这些最佳单因素组合(不包括碳氮源和C/N结果)培养木霉T-33绘制了产孢曲线，发现在培养第10d得到最大的厚垣孢子产量3.05×10~7个/ml。

Endophytic fungus of strain 2B isolated from Celastrus angulatus has been studied on the taxonomy,agricultural bioactivities, isolation and identification of active components, breeding of high yield strains,and optimization of fermentation conditions in this paper.

本论文以一株编号为 2B 的苦皮藤内生真菌为出发菌株，应用形态学的方法鉴定了其种属地位；研究了其代谢产物的农用生物活性，并对目标活性物质进行分离和结构解析，采取诱变育种手段选育了高产菌株，并对高产菌株的发酵条件进行了优化。

It was then introduced by gene pulser into acrystalliferous strain CryB and a recombinant strain CryB (pBMBZGC10) was obtained. Different fermentative solutions of recombinant strain were used for multi-spray on leaves of Brassica pekinesis, Ipomoea aquatica and Lycopersicon esculentum. Results by using fluorescent detection and PCR amplification revealed that cry1Ac10 gene could not been detected in roots, stems and leaves of plants tested. The cry1Ac10 gene was also not transferred into indigenous bacteria, actinomyces and fungi in soil.

苏云金芽胞杆菌标记重组菌株与杀虫基因水平转移的研究将重组菌株CryB（pBMBZGC10）的发酵液按浓度梯度分次进行喷洒小白菜、蕹菜和番茄作为供试植株，利用标记基因研究的结果表明：cry1Ac10基因没有向供试土壤细菌、真菌和放线菌转移，也未在植物的供试植物根、茎和叶中检测到该基因。

The thesis studied the total exocellular proteins, reductant sugar and the pectinase enzyme activity of CXJZ95-198 strain in glucose, mannose, xylan, mannosan and pectin culture media.Compared enzyme activity of pectinase, xylanose and β-mannanose. The secreted proteins in ferment fluid from different culture media were analysed by SDS-PAGE electrophoresis.

本论文对CXJZ95-198菌株在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中胞外酶蛋白质总量、还原糖及果胶酶活性的影响进行了研究；对果胶酶、木聚糖酶及β-甘露聚糖酶在甘露糖培养基中的酶活进行了比较；对CXJZ95-198菌株在不同碳源培养基中的发酵液在SDS-PAGE电泳中分泌出的蛋白带作了比较；对CXJZ95-198菌株所产生的胞外酶系中果胶酶进行了分离纯化。

Jones B A%Satter L D%Muck R E Influence of bacterial inoculant and substrate addition to lucerne ensiled at different dry matter contents 10.1111/j.1365-2494.1992.tb02243.x Grass and Forage Science , 1992,1

张涛%崔宗均%李建平%杨立国%康玉凡%胡跃高不同发酵类型青贮菌制剂对青贮发酵的影响草业学报， 2005，3

The new yeast strain HU-KDF-185 screened from interspecific fusants between Saccharomyces cerevisiae and S.diaslaticus demonstrates a high-yield producer of ethanol, The strain produces 30.77% of ethanol on account of fermented materials when it ferments saccharified liquid (about 5.0-5.5 Brix) prepared from dried sweet potato-residuces after making starch in the productive fermentation test of Xian Ju distillery.

从原生质体融合获得的K氏酵母(Saccharomyces cerevisiae var.ellipsoideus)和糖化酵母的种间融合杂种中筛选到一株酒精高产菌株HU-KDF-185，它在番薯渣糖化液(约5.0—5.5Brix)的工厂生产性发酵试验中，原料的酒精得率平均是30.77％；而当前使用的酒精生产菌株K氏酵母(对照1)则是29.71％，新市一号菌株(对照2)则是29.54％。

2The total living bacteria quantity in the fermented feedstuff was close to thecontrol(P＞0.05),but the coliforms and putrefying microbe were inhibited infermented feed by EM.

1EM中的优势菌群为乳酸杆菌，其次是酵母菌；EM发酵饲料中的优势菌也为乳酸杆菌，大肠杆菌、腐败菌受到明显抑制；EM能增加粪便中乳酸杆菌数量，降低沙门氏菌的检出率。

Compoound 15, 16 and 17 showed strong bioactivites against Bacillus subtilis, Candia albicans, Aspergillus niger and Tricophyton rubrum, which may also be the toxic substances to human skin.

体外抗菌作用研究表明化合物15、16和17具有强的抗菌作用，可能是该真菌发酵EtOAc提取物具有抗菌作用的物质基础，这3个化合物还可能是该提取物具有致皮肤过敏的物质基础。

This paper describes the selection of high producing strain?the optimization of fermentation process and studies of its fermentation kinetics, method for investigating the curdlan concentration with Congo red was established.

本文主要就凝胶多糖高产菌株的选育，发酵工艺条件优化和摇瓶发酵动力学进行了研究，建立了刚果红显色测定方法。

The optimum culture medium components of DA07405 contained glucose 1.0%, sucrose 0.5%, corn powder 0.5%, soybean powder 0.75%, K2HPO4 0.05%; The optimum fermentation conditions of DA07405 were initial pH7.0, seed age 48h, 12% inoculation amount, the size 75mL in 250mL Erlenmeyer flask at 200 r/min and 28℃ for 5d.

确定菌株DA07408的最佳培养基配方及发酵条件为：葡萄糖1.0%，甘油1.5%，玉米粉0.75%，大豆粉0.75%，K2HPO4 0.05%，初始pH值7.0，种龄48h，接种量10%，200r/min发酵5d，培养温度28℃，250mL三角瓶装液量75mL。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！