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This research has used the different origin Bacillus licheniformis strains, and the in-depth liquid fermentation production bacitracin and has studied the different strains, different carbon sources, different commensurability zinc and phosphorus to bacitracin high production effect.

采用细胞融合技术得到的菌株与不同来源地衣芽孢杆菌菌株,进行深层液体发酵生产杆菌肽,研究了不同菌株,不同碳源,不同量锌和磷量诸因素对杆菌肽高产的影响。

The size of bacteria,characteristic of stain,move about under light microscope and types of endospore were observed.

通过观察实验菌株的形态、染色特征、动力、芽孢的形态与位置,结合葡萄糖、阿拉伯糖、乳糖、肌醇发酵、卵磷脂试验、尿素水解试验、溶血性试验、青霉素抗性试验、溶菌酶抗性试验等,结果3种微生物被鉴定为蜡样芽孢杆菌、克柔念珠菌、枯草芽孢杆菌。

On the basis of Saccharification of the liquid component analysis and processing of corn stalk remnants of the scanning electron microscope on the surface of the micro-structure .

在对糖化液成分分析及预处理后的玉米秸秆进行了扫瞄电镜的表面结构的微观分析基础上,进行了玉米秸秆糖化液的发酵前预处理和秸秆糖化液的发酵条件,以及构建双菌株发酵体系进行了研究。

And unrooted trees were established. The accession numbers in GenBank of the sequences of 16S rDNA of the strains were followed by PFK1(DQ295034), PFK2(DQ295035), PFK3(DQ295036), LZCL(DQ295040), ZCS1(DQ295037), MMA1(DQ295041), MMA2(DQ295038), JSQ1(DQ295042), JSQ2(DQ295039), JSQ3(DQ295043). The Blast and phylogenetic tree analysis showed that

3将分离菌株进行形态、生理和糖发酵实验,结果表明这些表型鉴定的结论与16 S rRNA基因序列同源性分析结论一致,分离纯化5种产品的10株菌中仍有相同的3株菌与标签标注的不一致,即其中标签上分别标注为青春双歧杆菌和长双歧杆菌的两株菌鉴定结果均为动物双歧杆菌

The biological characteristics study of the Y9601and Y9801 strains showed the strain Y9601 had a stronger ability to assimilate starch and ferment alcohol from starch. The Y9601 was identified as a new species of Saccharomyces diastaticus, and the Y9801 was identified as Hansenula anomala.

对菌株Y9601及Y9801进行了生物学特性研究,表明菌株Y9601同化淀粉能力强,并对淀粉具有较强的酒精发酵能力,鉴定为酵母属糖化酵母的一个新种;菌株Y9801鉴定为异常汉逊酵母。

This paper focused on the breeding of an L-lactic acid producing bacteria Lactobacillus easei YBQ1-1, studying the characteristics, medium components, pH and metabolic network of the mutant YBQH2-14 obtained after being selected.Using UV irradiation, L.casei YBQH2-14 was selected in the different plate media such as the medium with high concentration of glucose, lactic acid , CaCO3 -Bromocresol purple and KMnO4 -KBr.

本文主要对L-乳酸生产菌株(Lactobacillus casei YBQ1-1)进行了诱变选育,并研究了突变株YBQH2-14发酵条件对生产L-乳酸的影响,构建了L.caseiYBQH2-14的代谢网络模型,分析了分批发酵不同阶段的代谢通量,同时研究了L-乳酸的应用——黑麦麸皮粉发酵。

A combination analysis of SIP and in situ microbiological methods was applied on the anaerobic digestion of biomass organics suffering from severe acids inhibition. The studies found that accumulated acids were degraded mainly by Methanosarcina firstly with acetoclastic pathway and then with hydrogenotrophic pathway under mesophilic condition, whereas acids were mainly degraded to methane by the tandem reactions of syntrophic acetate oxidation and hydrogenotrophic methanogenesis under thermophilic condition.

联合应用稳定同位素表征技术和微生态原位表征方法,对高浓度有机酸胁迫条件下的生物质类有机物厌氧发酵甲烷化途径的研究结果表明,面临高浓度有机酸迅速累积的环境,中温发酵条件下,优势菌种为甲烷八叠球菌,依时间次序,通过乙酸发酵和氢营养型途径利用累积的有机酸产生甲烷;而在高温条件下,有机酸则通过乙酸氧化和氢营养型甲烷化途径的串联反应被降解。

Taking Eremothecium ashbyii (abbreviated as Eaw95. 1) as the original strain, after Υ-ray mutation, tubercidin and 8--azaguaniae (8--AG) resistant tests, a strain labeled as T〓 capable of overproducing riboflavin was obtained and its genetic stability was analyzed. Compared with Eaw95. 1, the riboflavin productivity was improved about 40% by flask--shaking tests.

以Eremothecium ashbyii为出发菌(间称Eaw95.1),通过Υ-射线诱变,杀结核菌素和8-氮杂鸟嘌呤等结构类似物的的抗性筛选,获得了一株核黄素高产菌T〓,并对菌株的遗传稳定性进行了研究,获得了较好的效果,与出发菌株相比,核黄素的摇瓶发酵产量提高了近40%。

Times higher than that of initial strain. Polysaccharides, such as barley flour, dextrin and soluble starch, were better carbon sources than monosaccharides and disaccharides, such as glucose and maltose, for cell growth of B subtilis ZJF-1A5 and β-glucanase production. Yeast extract was the best nitrogen source, the following was soybean flour.

糊精、大麦粉等多糖有利于B.subtilisZJF-1A5 β-葡聚糖酶的产生,葡萄糖、蔗糖等易利用性碳源不利于菌体生物量的积累和β-葡聚糖酶的产生,β-葡聚糖酶受分解代谢产物阻遏调节。B.subtilis ZJF-1A5 β-1,3-1,4-葡聚糖酶的产生与菌体生物量和菌体生长状态密切相关,提高发酵产酶水平的关键是提高菌体生物量和解除葡萄糖效应。

The results indicated that the regulation of the acidity of raw juice could prevent bacteria contamination and prolong 2~3 d shelf period under homothermal condition (temperature at 29 ℃), the addition of active dry yeast with high temperature tolerance (addition level as 0.1g/100 mL) could realize thick mash fermentation, the fermentation time should be controlled about 28 h, and sugar content of fermenting mash was 20.6 g/100 mL and the alcohol content of matured mash reached 12.8 %vol.

结果表明,调整原汁的酸度,能够防止杂菌污染,在29 ℃恒温条件下,可延长甜高粱榨汁的保存期至2~3 d;耐高温活性干酵母的加量为0.1 g/100 mL,并可实现甜高粱秸秆榨汁的浓醪发酵;发酵时间控制在28 h左右;高糖度发酵醪含糖量为20.6 g/100 mL,成熟醪酒精度达12.8 %vol。

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