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Methods Rabbits were administered by intravaginal amoxicillin and metronidazole solution, then the experimental vaginitis model were established by E. coli and Staphylococcus aureus, were treated with lactobacilli lysates, lactobacilli lysates in lower concentration, lactobacillus fermented broth, the probiotic lactobacillus and N. S for 7 days respectively. The variety in vaginal microecosystem were analysed and investigated.

用阿莫西林和甲硝唑接种家兔建立脱污染模型,再用大肠埃希菌和金黄色葡萄球菌的混合液接种,建立实验性感染模型后,随机分为5组,分别用乳杆菌裂解物原液、裂解物稀释液、发酵上清液、乳杆菌活菌制剂及生理盐水进行治疗,分析和考察阴道菌群变化。

In order to increase the density of cell and give more guides to optimization of batch fermentation, a Study on the characteristics of Alcaligenes faecalis should be carried out.

为了提高菌体阶段菌体的生长密度、对分批发酵有一些优化指导作用,对菌体本身特性进行了研究。

There were 70 strains isolated from each of the MRS and VJ at the different stage of the processing of dried beef. Staphylococcus warneri, Staphylococcus sciuri, Staphylococcus auricularis, Bacillus subtilis, Staphylococcus xylosus, Staphylococcus saprophyticus, Lactobacillus plantarum and Pediococcus pentosaceus were 8 predominate bacteria. The growth curve of predominate bacteria was studied. The result showed that growth of Pediococcus pentosaceus was faster than that of Lactobacillus plantarum; the growth of Staphylococcus sciuri is fastest and Staphylococcus auricularis was slowest.

在牛肉自然发酵加工过程中的不同阶段,利用MRS和PCA~+培养基分离到乳酸菌和微球菌各70株,经过分析鉴定,得到8株优势菌,分别为沃氏葡萄球菌、松鼠葡萄球菌、耳氏葡萄球菌、木糖葡萄球菌、腐生葡萄球菌、枯草芽孢杆菌、植物乳杆菌和戊糖片球菌。

Strain Ppl35 can secrete human lysozyme in media when inducing by methanol, so it can be determinated for human lysozyme bacteriolytic activity straightly in fermentative supernatants by agar plate diffusion method which used Microccous lysodeikticus as the test bacterium.

菌株Pp135经甲醇诱导,可将人溶菌酶分泌到培养基中,因此可在发酵上清液中直接检测到人溶菌酶的活性。

In this research work, the technology of microbial conversion was studied in order to establish an environment friendly method for the production of doxorubicin. A doxA gene encoding Daunorubicin C-14 hydroxylase was cloned from a daunorubicin-producing strain Streptomyces coeruleorubidus SIPI 1482. Some plasmids for the expression of doxA gene were constructed and transformed into S.

本研究对微生物转化法替代现有工艺生产阿霉素进行了探索,从柔红霉素产生菌天蓝淡红链霉菌SIPI 1482中克隆了柔红霉素C-14羟化酶基因,构建了doxA基因的链霉菌表达质粒,导入变铅青链霉菌TK24中获得了柔红霉素转化基因工程菌,并研究了doxA基因在工程菌中的表达,最后还对工程菌转化柔红霉素生成阿霉素的发酵工艺进行了初步研究。

Result The phytopathogenic fungus Pythium aphanidermatum was isolated; 7 strains of antagonistic actinomycete, which had obvious inhibitory effects on Pythium aphanidermatum were screened. The inhibitory ring of No.8 strain was 13.3 mm, and reached 88.3% when cultivated for 120 h.

伽师甜瓜猝倒病的病原菌为瓜果腐霉;筛选出7株对瓜果腐霉具有显著拮抗性的生防放线菌,其中8号放线菌对瓜果腐霉的拮抗环宽度为13.3mm,其无菌发酵滤液对瓜果腐霉的抑菌率在培养120h时高达88.3%。

Soaking seed of rice and wheat with zymolytic liquid of Trichoderma harziaiarum, Trichoderma DWC and Trichoderma viride, comparing with germinate ratio, germinate force, root length, bud length and ratio of root to bud, the results show that 50 times dilute zymolytic liquid of Trichoderma harziaiarum has a relatively notable promote role for germinate of seed .The effect of Trichoderma viride to germinate of seed is not evidence. Trichoderma DWC for seed sprout has certain inhibition.

据此,采用哈茨木霉作为出发菌株,通过离子束诱变,从大菌落株中筛选出促进生长的高产突变株——h-13菌株,通过对用该菌株发酵液处理后的水稻及小麦的种子的各项生理生化指标的测定,发现该菌株比其它菌株更能促进水稻和小麦的活力和发芽率,三天时小麦的总根长为3.03cm,比对照的1.42cm的两倍还多,并且能够明显提高种子发芽后的淀粉酶和蛋白酶的活力,其活力都是对照的两倍左右。

A set of models including cell growth, product formation and substrate consumption was developed according to different peculiarities of cell growth and PHB synthesis. This was found to agree well with the experimental data.

根据PHB分批发酵过程中菌体生长和产物形成阶段的不同特点建立的分批发酵动力学模型能较好地用来分批发酵中各参数的变化。

Based on the plant growth rate and inhibition zone methods, the antifungal activity of extracts of every organ of Xanthium sibiricum, the fermentation liquid of endophytic fungi MG4-23, and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively. The extract of every organ of X.

方法]采用生长速率法和抑菌圈法,分别以水稻纹枯病菌等6种植物病原真菌和大肠杆菌等3种病原细菌为供试菌种,对苍耳各器官提取液、植物内生真菌MG4-23的发酵液及菌丝体提取液进行抑菌活性测定。

In this paper, adopted the method of microorganism pour plate, 28 strains of filiform fungi were isolated from edible fungi waste of our school .Kaolinite suspension was flocculated by the culture broth ,which was regarded as the index to determine flocculating activity and ability to produce flocculant. 10 flocculant-producing strains which had higher flocculating activity were obtained and they were further rescreened.

以发酵液对高岭土悬浮液絮凝效果为指标衡量其絮凝活性及产絮凝剂能力,经过初筛从分离株中筛选出10株具有较高活性的絮凝剂产生菌,并对这10株菌株进一步作复筛试验,最终选出其中一株絮凝剂高产菌暂时定名F_

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