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The mutants were further screened through repeated fermentations as well as the measurement of their physical properties. The results showed that some major fermentation indexes of strain HJ1615-d4 such as alcoholicity, fermenting power,total acids and flavoring components etc. were almost the same as those of the original strain. However, its urea yield was only 15.41 mg/L, louer 50 % than that of the original strain.

对这4株突变株进行发酵栓实验,并测定其发酵综合性能,其中菌株HJ1615-d4在发酵力、总酸、酒精度以及风味物质等综合指标方面保持了亲株的优良性状,同时产尿素的量为15.41 mg/L,低于出发菌株产尿素量50 %以上。

The experiment optimized the culture medium and conditions for PV 1 to yield the highest GABA: sucrose as carbon source,peptone and beef extract as complex nitrogen source,initial pH at 7.0,temperature at 30~34 ℃.The content of GABA was over 4 g/L in the optimum culture conditions.Thereby,i...

实验还对酸菜1号菌株的培养基和培养条件进行了优化:用蔗糖为碳源、蛋白胨和牛肉膏作为复合氮源、培养基初始pH为6.0、培养温度为30~34℃时该菌株发酵产GABA的量最多,发酵过程中的振荡培养(100 r/min)和静置培养对GABA的产量无明显影响;在最佳培养基组合和发酵条件下,发酵液中GABA含量可达4 g/L以上,表明利用乳酸菌进行富含GABA食品的生产是可行的。

The results showed: no significant difference was found between the results of single tube quantitative assay method and multiple tube fermentation technique;single tube quantitative assay method showed better accurateness,precision,and reproducibility than multiple tube fermentation technique;single tube quantitative assay method was quicker and more economical.

结果表明:MUG单管定量检测法与大肠埃希氏菌多管发酵法对菌悬液、天然海水、水产品的检测结果没有统计学差异;MUG单管定量检测法检测数据的精确性高于大肠埃希氏菌多管发酵法,而且更为快速、经济。

Results show that the antibacterial component belongs to volatile oil、sesquiterpene、phenols and organic acids. The antibacterial part of the broth was isolated by silica gel column and 6 fractions of obviously antibacterial activity were obtained .By further study Fractions 1、2、3 contain sesquiterpene and fractions 8、9 contain phenols and fraction 10 contains phenolic organic acid.

对发酵液抑菌成分的研究表明,抑菌活性成分组成为挥发油倍半萜、酚和有机酸等类化合物,采用硅胶柱层析对发酵液抑菌活性部位进行分离实验,得到了6个具有明显抑菌活性的粗组分(组分1、2、3、8、9、10),其中组分1、2、3含有倍半萜类物质,组分8、9含有酚类物质,组分10中有含酚羟基的羧酸类物质。

Using assay of 21 Wistar rats from diarrhea intestine separation, pure culture of bacteria in the intestine containing 1% were small thorn Hericium erinaceus polysaccharide fermentation broth and 1% fermentative polysaccharide liquid medium 37 ° C for 18 h after the OD value, Statistical analysis showed Hericium and see 21 species of Ganoderma intestinal bacteria strains of the five species of a clear inhibitory effect other strains of the role is not obvious, Ganoderma and Hericium compared Hericium no significant role in the strain Ganoderma and there are obvious inhibition, Hericium and prominent role in the strain.

采用比浊法测定了21种从腹泻Wistar大鼠肠道内分离、纯培养的肠道细菌在分别含有1%小刺猴头菌发酵液多糖和1%灵芝发酵液多糖的培养基中37℃培养18h后的OD值,经统计分析结果表明见猴头和灵芝对21种肠道菌中的5种菌株有明显的抑菌作用,而对其他菌株的作用并不明显,灵芝和猴头相比较,猴头没有显著作用的菌株,而灵芝却有显的抑制作用,而猴头具有显著作用的菌株。

Each of three lactic acid bacteria from EZAL-MY96 was cultured and fermental techniques were studied by experiments with 5L fermentive vessel. Technique parameters of three lactic acid bacteria were determined.

对EZAL-MY96直投式酸奶发酵剂中分离出来的3株乳酸菌利用5L发酵罐进行发酵工艺研究,确定3株菌中每一菌株的最佳发酵工艺参数。

In high dentity fermentation, the cellular respiration, energy metabolism, highest bacterial density and dry bacteria weight of the G830 strain were markedly better than control strains PA1 and BL21, The expression of recombinant prolyl endopeptidase in G830 and PA1 under the above condition was high and stable. Their growth situation and fermentation parameters were similar with their parental strains without plasmids, and resided plasmids maintained stably in those strains.

在高密度发酵条件下,G830的细胞呼吸强度、能量代谢、最高菌密度和细胞干重,均明显优于对照菌株PA1和BL21;重组蛋白脯氨醯内肽酶在G830和PA1中获得稳定高表达;重组菌生长状况及发酵指标均与空宿主菌基本一致且表达质粒能维持较好的稳定性。

During the stationary phase of fermentation, cell productivity was slightly reduced. MgSO_4 and tryptone have the positive effect on the product synthesis and cell growth respectively. Based on these conclusions, MgSO_4 and tryptone were added to the feed medium to enhance the production of polysialic acid. Variable feed rate policies were applied to the fed-batch fermentation process and the final biomass and polysialic acid production were both increased by 40 percent.

通过摇瓶实验研究了大肠杆菌发酵过程中菌体生长和产物合成的影响因素,结果表明:产物合成与菌体生长是相耦联的过程,菌体生长受到菌体代谢产物的抑制作用,聚唾液酸的合成不受代谢产物的抑制作用;发酵进入稳定期后菌体的生长和生产能力有所降低;硫酸镁和胰蛋白胨分别对产物合成和菌体生长有一定的促进作用。

Saccharified bran was produced by thick layer and aeration solid fermentation of Aspergillus niger HS-16 with bran as raw material.

利用黑曲霉Aspergillus niger HS-16菌株作生产菌,麸皮为原料,采用厚层通风固体发酵工艺糖化麸皮,使麸皮的还原糖含量从2.8g/100g增加到糖化后的39.0g/100g,为红曲霉M-1菌株利用麸皮制备红曲麸增加了可发酵碳源。

The results showed that the fermentation liquid from A44 showed strong inhibitory effect on mycelium growth and on spore germination with tower germination rate against pathogenic funguses Alternarina.alternate .

结果表明A44菌株发酵液对烟草赤星病菌、黄瓜枯萎病菌、西瓜枯萎病菌等病原菌有抗菌活性及抑制孢子萌发作用,且这种作用在A44发酵液稀释了160倍后仍然存在;对峙培养试验表明A44菌对植物病原菌有很强的拮杭作用,拮抗机制主要为营养竞争作用、抗生作用等。

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