发酵菌的

Forty-seven strains of bacteria were isolated from the samples of Kumiss products collected from the pastoral area in Xilin Guole,and its biological properties were studied.

本项研究主要以内蒙古锡林郭勒牧区马奶酒发酵菌冻干粉为样品，进行了马奶酒发酵菌的活化、分离、纯化、鉴定及其生物活性的研究。

To investigate the mechanisms of biohydrogen production by anerobic fermentation of solid organic waste, gas chromatography is used to analysis the biogas and volatile fatty acid in anaerobic bio-reactor. Firstly, the ability of hydrogen production by digested sludge from the West Lake, Enteobacter aerogences, digested sludge from sewage farm, the fluid of methane pool and dejecta have been studied. The rule of the ability of hydrogen production by different bacteria under different control condition. Simultaneity, the importance of the synergistic effect on hydrogen production has been proved.

本文首先选用西湖底的厌氧活性污泥、产气肠杆菌、污水处理厂的淤泥、沼气池发酵液以及猪粪等不同菌群对废弃食物——马铃薯进行厌氧发酵产氢特性实验研究，得到马铃薯在各不同菌群及工况下的发酵产氢能力，同时发现产氢菌间的协同作用很重要，在控制好发酵条件的情况下，产氢菌群发酵通常会好于单一产氢菌的发酵。

Shandong Shengli Bioengineering Co., Ltd., Jining 272037, Shandong, China Abstract: The cause of infection in the process of avermectin production was studied from infected time, scope and bacterial type. The test method to check the infection caused by other bacteria was also raised. The ways to prevent infection was ascertained from preparation of microbial strain, air purification, medium disinfection, ductwork deployment and systematic dead angle cleaning. Meanwhile, adjustment measure was summarized in different infected period such as microbial strain, seed pot and yeastiness.

山东胜利生物工程有限公司，山东济宁 272073 摘要：从染菌的时间、类型、范围三方面探讨了阿维菌素发酵染菌的原因，提出了发酵过程中杂菌的检查方法，分别从菌种制备、空气净化、培养基灭菌、管路配置、系统死角清理等方面明确了防止发酵染菌的措施，得出了斜面菌种、种子罐、发酵罐不同时期染菌后的补救措施。

OBJECTIVE To investigate the clinical distribution and drug resistance of non-fermentative bacilli isolated from department of surgery so as to guide the rational use of antibiotics.

目的了解医院外科非发酵菌的临床分离状况及常见菌株的耐药性，指导临床合理选用抗菌药物。

Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群，序列同源性大于99%，与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6，6~18，18~66和66 h；培养12 h后，YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56，此后逐渐上升，至发酵结束时其pH值分别为7.74和8.07；培养0~18 h时2菌株发酵液中还原糖含量迅速降低，此后保持稳定；12 h时氨基氮含量达到最低，此后开始缓慢上升；YL001菌株培养54 h后及YL002菌株培养42和66 h后，其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。

By comparing with the curves of classical antibiotics in Doskochilova system, the antibiotic type of fermentation broths was identified. The antimicrobial tests showed, the inhibition rate of alt the twenty strains were considerably and ten of them reached 80% when the fermentation broths was diluted by 10 times. It showed strongest inhibition effect against Erwinia carotorora (17.75 mm). Organize method tests showed that inhibition rate of fermentation broths of the strain HJ1-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100% and 71.39%, respectively.

发酵液的抑菌活性测定结果表明，放线菌HJ1-2菌株发酵液对20种供试病原真菌均具有不同程度抑制作用，其10倍稀释液对其中10种病原真菌菌丝生长的抑制率达到80%以上；供试7种细菌中放线菌HJ1-2菌株发酵液对白菜软腐病菌的抑制作用最强，抑菌圈直径为17.75mn；放线菌HJ1-2菌株发酵液对油菜菌核病和番茄灰霉病的抑制率分别为100和71.39%。

4Mm harvested in preculture, is the best. In this form, Rhizopus oryzae R1021 produced most L-lactic acid (76. 62g/L), the pellet size in production medium in flask and fermentor are 1. 9mm and 2. 6mm, respectively, and its L-lactic acid productivity reached or exceeded that of the immobilized cell with polyurethane foam. The produced L-lactic acid and the yield of L-lactic acid based on the total glucose and the productivity are above 74g/L and 74% and 2. 69g/L h, respectively, in 7 batches repetitive fermentation in fermentor, and the size of pellets remain 2. 6mm～3. 4mm.

摇瓶种子培养获得的菌球直径为1.4mm时，这种大小的菌球产酸最高，为76.62g/L；研究表明以直径1.4mm的菌球为种子，在摇瓶发酵和发酵罐发酵中菌球大小分别为1.9mm和2.6mm；这种菌球的产酸水平最高，可以达到甚至超过聚氨酯泡沫载体固定化细胞发酵乳酸的水平；在反复7批次发酵罐发酵过程中，菌球的大小始终保持在2.6mm～3.4mm之间，L-乳酸产量、得率和生产能力分别保持在74g/L、74％和2.69g/L h以上。

First, the fermentation of residue with Saccha romyces cerevisia was studied. In the process of fermentation, the effects of temperature, initial pH, inoculums size, carbon and nitrogen addition , cultivation time were discussed.

通过对残渣成分的分析，以及温度、初始pH、接菌量、添加C、N源及发酵时间等对单菌发酵残渣时啤酒酵母生长的影响，得出：在35℃、初始pH6.0、接菌量10%、发酵24h的条件下，粗蛋白含量由发酵前的5.3%提高到发酵后的6.5%，提高幅度为22.6%。

Lactic acid fermentation is efficient, and the fermenting organisms rapid in growth.

乳酸发酵的效率高，发酵菌的生产迅速。

The effect of temperature, inoculums size, fermentation time, n...

通过对残渣成分的分析，以及温度、初始pH、接菌量、添加C、N源及发酵时间等对单菌发酵残渣时啤酒酵母生长的影响，得出：在35℃、初始pH6.0、接菌量10%、发酵24h的条件下，粗蛋白含量由发酵前的5.3%提高到发酵后。。。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。