发酵菌

The results showed that the Mulberry fruit surface carried large numbers of yeast but little other mixed fungi; ethanol fermentation reached the peak value in 48h'; before vinegar production the acetification bed breeding took 9~10d, but the vinegar fermentation cycle was only about 24h during production .

结果表明，桑椹果实表面携带有大量的酵母菌而几乎不带其他杂菌，酒精发酵醪除含有大量的酵母菌之外未发现有其他杂菌，酒精度在发酵 48h 时达到高峰；桑椹醋生产前的醋酸菌床培育需要9～10d 时间，投入生产后醋酸发酵周期仅需要24h ；桑椹醋总黄酮含量高达6.68 mg/mL ，对 DPPH 自由基的清除作用远高于山西陈醋、镇江香醋和山楂果醋，具有良好的抗氧化作用；利用桑椹醋自身色素颜色变化可判断酸度测定时酸碱滴定终点。

The results showed that the mulberry fruit surface carried large numbers of yeast but little other mixed fungi; ethanol fermentation reached the peak value in 48h'; the acetification bed breeding took 9~10 d before vinegar production, but the vinegar fermentation cycle was only about 24h during production.

结果表明，桑椹果实表面携带有大量的酵母菌而几乎不带其他杂菌，酒精发酵醪除含有大量的酵母菌之外未发现有其他杂菌，酒精度在发酵48h时达到高峰；桑椹醋生产前的醋酸菌床培育需要9~10d，投入生产后酷酸发酵周期仅需要24h；桑椹醋总黄酮含量高达6.68mg/mL，对DPPH的清除作用远大于山西陈醋、镇江香醋和山楂果醋，具有良好的抗氧化作用；利用桑椹醋自身色素颜色变化可判断酸度测定时酸碱滴定终点。

In order to find new kinds of antibacterial agents, a primary screening model has been set with the DNA gyrase B subunit as a target, and then the active compounds of positive strain were purified. From Mycobacterium phlei, we isolated a novobiocin supersensitive mutant M.phlei SS12 and a resistant ~mutant M.phlei RR11 with NTG and UV.

实验采用Mycobacteriumphlei为出发菌株，利用NTG和紫外线复合诱变得到新生霉素特异性的超敏菌株M.phleiSS12和耐药菌株M.phleiRR11，建立了以DNA回旋酶B亚基为靶点的抗结核药物的筛选模型；并应用此模型对1600株真菌和100株放线菌发酵液进行了初筛，获得了38株阳性菌，阳性率为2.4%；对阳性菌株3277的发酵液进行了提取分离，得到X1和X2两个活性化合物。

Better results could be obtained, For 40 hours fermentation, cell dry weight and PHB concentration reached 32.7 g · L-1 and 21.4g · L-1 respectively.The synthesis of Poly (β-Hydroxybutyrate-co-β-Hydroxyvalerate) by the strain G-IIIy from different precusor was studied. It was found that the strain G-IIIy could accumulate PHBV with sucrose as carbon source and propionic acid or valeric acid as precursor . In 2L self-controlled fermentor, the dry cell weight, PHBV concentration and PHBV content reached 35.8g·L-1, 22.6g·L-1 and 38.4% respectively in the case of fermentation for 42 hours and the propionic acid as precursor.

研究了添加不同前体物，G-Ⅲy菌株生产聚β-羟基丁酸和聚β-羟基戊酸共聚物的发酵条件，结果表明，此菌株能以丙酸或戊酸为前体，在蔗糖为碳源的条件下合成PHBV共聚物；在2L发酵罐中，以丙酸为前体，发酵16小时开始流加丙酸，根据发酵液pH值变化控制丙酸流加量，发酵42小时，细胞干重、PHBV产量和含量分别达到35.8g/L，22.6g/L，63.13％。

The actinomyces was idicntified as a new species of the Microsircplosporu Its antifungal spectrum 024-antibiotic includes many plantpathogenic fungi, such as Glomerella cingulata, Fusarium gramiaearum, Helmmthosporiuin sativitnt,/iotrvosphticriu dothidea, Alternaria xolani, Endothia parasitica etc..

本文针对防治小麦纹枯病的上百种生防放线菌进行筛选后，选择出目标菌株024，随后对它的菌种的初步鉴定、初始发酵条件与最适发酵条件进行了探索，并对024放线菌所产抗生素的纯化、理化性质、发酵液的防治效果等内容进行了研究。

Using assay of 21 Wistar rats from diarrhea intestine separation, pure culture of bacteria in the intestine containing 1% were small thorn Hericium erinaceus polysaccharide fermentation broth and 1% fermentative polysaccharide liquid medium 37 ° C for 18 h after the OD value, Statistical analysis showed Hericium and see 21 species of Ganoderma intestinal bacteria strains of the five species of a clear inhibitory effect other strains of the role is not obvious, Ganoderma and Hericium compared Hericium no significant role in the strain Ganoderma and there are obvious inhibition, Hericium and prominent role in the strain.

采用比浊法测定了21种从腹泻Wistar大鼠肠道内分离、纯培养的肠道细菌在分别含有1%小刺猴头菌发酵液多糖和1%灵芝发酵液多糖的培养基中37℃培养18h后的OD值，经统计分析结果表明见猴头和灵芝对21种肠道菌中的5种菌株有明显的抑菌作用，而对其他菌株的作用并不明显，灵芝和猴头相比较，猴头没有显著作用的菌株，而灵芝却有显的抑制作用，而猴头具有显著作用的菌株。

E isoated and obtained a excellent strain of producing 2 - keto - L - gulonic acid -Bdellovibrio J26. The fermentation condition of 2 - keto - L - gulonic acid(2 - KGA) wasstudied extensively. Addition some carbon source or nitrogen source in culture medium canincrease the 2 - KGA yield from 5O - 6Omg/ml to 7O - 76 mg/ml.

对新分离得到的能产生维生素C前体2-酮基-L-古龙酸（2-KGA）的优良菌株蛭弧菌J26，进行了一系列摇瓶发酵条件试验，通过添加一些其它有机碳源或其它氮源，可使蛭弧菌J26摇瓶发酵产生2－KGA由50-60mg／ml上升到70mg／ml以上，采用SMA探针技术的流加发酵摇瓶试验产生2－KGA可以达到83.9－91.7mg／ml。

And Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp.

该菌株的发酵液对大豆胞囊线虫二龄幼虫具有较高的毒杀活性并显著抑制胞囊孵化，不同稀释浓度发酵液的处理和无菌水处理有显著差异，发酵原液的杀线虫效果可达到90％以上，温室防效试验效果明显。同时该菌株对根结线虫Meloidogyne spp。

During the stationary phase of fermentation, cell productivity was slightly reduced. MgSO_4 and tryptone have the positive effect on the product synthesis and cell growth respectively. Based on these conclusions, MgSO_4 and tryptone were added to the feed medium to enhance the production of polysialic acid. Variable feed rate policies were applied to the fed-batch fermentation process and the final biomass and polysialic acid production were both increased by 40 percent.

通过摇瓶实验研究了大肠杆菌发酵过程中菌体生长和产物合成的影响因素，结果表明：产物合成与菌体生长是相耦联的过程，菌体生长受到菌体代谢产物的抑制作用，聚唾液酸的合成不受代谢产物的抑制作用；发酵进入稳定期后菌体的生长和生产能力有所降低；硫酸镁和胰蛋白胨分别对产物合成和菌体生长有一定的促进作用。

The medium composition and some fermentation parameters were studied by cross test and single factor experiments. The optimal conditions are as flows: Tryptone 0.76 %, Bean powder 1.0 %,initial pH 8.0, temperature 28 ℃, inoculation quantity 8 % and loading volume 30 %. Under optimal conditions, the maximum yield of biomass and microbial lipids reached 29.8 g/L and 11.7 g/L, respectively, which was 2.59 times and 2.05 times of those cultured in the original medium, after 9 days fed-batch co-fermentation in shake-flask.

采用单因子和正交实验对培养基组成和发酵条件进行了优化，结果表明，诱变菌在温度28 ℃，pH 8.0，接种量8 %，装液量30 %，蛋白胨0.76 %和豆粕1 %，分批补糖的最优发酵条件下发酵9 d，生物量和菌体油脂积累量分别达29.8 g/L和11.7 g/L，较出发菌提高了2.59倍和2.05倍，同时原料糖利用率达到99.4 %。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。