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In order to reach a higher productivity level of mycelium and exopolysaccharide ,L934 orthogonal experiments on fermentation with Schizophyllum commune were carried out in 7 L fermentor.

在7 L发酵罐上进行了裂褶菌发酵生产菌丝体和胞外多糖的研究,采用L_9(3~4)正交试验对发酵条件进行了优化。

Schizophyllum commune is a kind of edible and medicinal fungi,which contains different kinds of bio—active constituents,such as polysaccharides.

在7 L发酵罐上进行了裂褶菌发酵生产菌丝体和胞外多糖的研究,采用L_9(3~4)正交试验对发酵条件进行了优化。

A combination analysis of SIP and in situ microbiological methods was applied on the anaerobic digestion of biomass organics suffering from severe acids inhibition. The studies found that accumulated acids were degraded mainly by Methanosarcina firstly with acetoclastic pathway and then with hydrogenotrophic pathway under mesophilic condition, whereas acids were mainly degraded to methane by the tandem reactions of syntrophic acetate oxidation and hydrogenotrophic methanogenesis under thermophilic condition.

联合应用稳定同位素表征技术和微生态原位表征方法,对高浓度有机酸胁迫条件下的生物质类有机物厌氧发酵甲烷化途径的研究结果表明,面临高浓度有机酸迅速累积的环境,中温发酵条件下,优势菌种为甲烷八叠球菌,依时间次序,通过乙酸发酵和氢营养型途径利用累积的有机酸产生甲烷;而在高温条件下,有机酸则通过乙酸氧化和氢营养型甲烷化途径的串联反应被降解。

By means of mycelium growth rate, cup plate method and organize method, the antimicrobial activity of bioassay was studied. The stability of the fermentation broths in different conditions was measured.

采用菌丝生长速率法、管碟法和活体组织法测定发酵液的抑菌活性,在不同条件下测定发酵液的稳定性,捷克溶剂系统纸层析法鉴别发酵液中抗生素类型。

The Etest is an altenmative to agar dilution method for gonococci susceptibility testing to ceftriaxone,penicillin and ciprofloxacin due to its rapid,effectiveness and significant relationship with agar dilution method.

菌株来源实验菌株均从我所STD门诊患者的泌尿生殖道分泌物中分离得到,所有菌株经革兰染色、氧化酶试验和糖发酵试验证实为淋球菌传代一次,洗于脱脂牛奶中,于- 78℃低温冰箱冻存备用。2 。

Taking Eremothecium ashbyii (abbreviated as Eaw95. 1) as the original strain, after Υ-ray mutation, tubercidin and 8--azaguaniae (8--AG) resistant tests, a strain labeled as T〓 capable of overproducing riboflavin was obtained and its genetic stability was analyzed. Compared with Eaw95. 1, the riboflavin productivity was improved about 40% by flask--shaking tests.

以Eremothecium ashbyii为出发菌(间称Eaw95.1),通过Υ-射线诱变,杀结核菌素和8-氮杂鸟嘌呤等结构类似物的的抗性筛选,获得了一株核黄素高产菌T〓,并对菌株的遗传稳定性进行了研究,获得了较好的效果,与出发菌株相比,核黄素的摇瓶发酵产量提高了近40%。

Amino nitrogen content as protease enzyme capacity indicators was determinated in the fermentation process.

在酱油发酵中,研究了菌株HUV-2的发酵规律并对发酵条件进行探索。

Times higher than that of initial strain. Polysaccharides, such as barley flour, dextrin and soluble starch, were better carbon sources than monosaccharides and disaccharides, such as glucose and maltose, for cell growth of B subtilis ZJF-1A5 and β-glucanase production. Yeast extract was the best nitrogen source, the following was soybean flour.

糊精、大麦粉等多糖有利于B.subtilisZJF-1A5 β-葡聚糖酶的产生,葡萄糖、蔗糖等易利用性碳源不利于菌体生物量的积累和β-葡聚糖酶的产生,β-葡聚糖酶受分解代谢产物阻遏调节。B.subtilis ZJF-1A5 β-1,3-1,4-葡聚糖酶的产生与菌体生物量和菌体生长状态密切相关,提高发酵产酶水平的关键是提高菌体生物量和解除葡萄糖效应。

A set of models including cell growth, product formation and substrate consumption was developed according to different peculiarities of cell growth and PHB synthesis. This was found to agree well with the experimental data.

根据PHB分批发酵过程中菌体生长和产物形成阶段的不同特点建立的分批发酵动力学模型能较好地用来分批发酵中各参数的变化。

To explore the effect of coculturing actinomycetes with Bacillus subtilis on the production of bioactive secondary metabolites, we studied the difference between fermentation products of monocultures and the corresponding cocultures of 22 actinomycetes by antimicrobial assay and HPLC-PDA analysis.

为探讨共培养对放线菌产生活性次生代谢产物的影响,结合抗菌活性测定及HPLC-PDA分析,研究了22株放线菌的单培养及其与枯草芽孢杆菌的共培养发酵代谢产物的差异,并选取抗菌活性较强的链霉菌FXJ2.014进一步研究其代谢产物。

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