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And unrooted trees were established. The accession numbers in GenBank of the sequences of 16S rDNA of the strains were followed by PFK1(DQ295034), PFK2(DQ295035), PFK3(DQ295036), LZCL(DQ295040), ZCS1(DQ295037), MMA1(DQ295041), MMA2(DQ295038), JSQ1(DQ295042), JSQ2(DQ295039), JSQ3(DQ295043). The Blast and phylogenetic tree analysis showed that

3将分离菌株进行形态、生理和糖发酵实验,结果表明这些表型鉴定的结论与16 S rRNA基因序列同源性分析结论一致,分离纯化5种产品的10株菌中仍有相同的3株菌与标签标注的不一致,即其中标签上分别标注为青春双歧杆菌和长双歧杆菌的两株菌鉴定结果均为动物双歧杆菌

Technology of the honey- persimmon juice fermented drink were researched, and effect of sensory check by additive quantities of yeast and acetobacter with different temperatures and times of fermentation were investigated.

研究了蜂蜜、柿子汁发酵饮料的制作工艺及酵母菌、醋酸菌接种量、醋酸发酵温度和发酵时间对产品感官质量的影响。

The optimal conditions for acetic fermentation were at 30℃ for 8 d with the initial concentration of 8% alcohol and 12% inoculum of Acetobacter...

醋酸发酵最佳工艺条件为发酵温度30℃,醋酸菌接种量12%,初始酒精度8%,发酵8d。酿制出的红树莓果醋香味独特,品位纯正。

This paper focused on the breeding of an L-lactic acid producing bacteria Lactobacillus easei YBQ1-1, studying the characteristics, medium components, pH and metabolic network of the mutant YBQH2-14 obtained after being selected.Using UV irradiation, L.casei YBQH2-14 was selected in the different plate media such as the medium with high concentration of glucose, lactic acid , CaCO3 -Bromocresol purple and KMnO4 -KBr.

本文主要对L-乳酸生产菌株(Lactobacillus casei YBQ1-1)进行了诱变选育,并研究了突变株YBQH2-14发酵条件对生产L-乳酸的影响,构建了L.caseiYBQH2-14的代谢网络模型,分析了分批发酵不同阶段的代谢通量,同时研究了L-乳酸的应用——黑麦麸皮粉发酵。

In this research work, the technology of microbial conversion was studied in order to establish an environment friendly method for the production of doxorubicin. A doxA gene encoding Daunorubicin C-14 hydroxylase was cloned from a daunorubicin-producing strain Streptomyces coeruleorubidus SIPI 1482. Some plasmids for the expression of doxA gene were constructed and transformed into S.

本研究对微生物转化法替代现有工艺生产阿霉素进行了探索,从柔红霉素产生菌天蓝淡红链霉菌SIPI 1482中克隆了柔红霉素C-14羟化酶基因,构建了doxA基因的链霉菌表达质粒,导入变铅青链霉菌TK24中获得了柔红霉素转化基因工程菌,并研究了doxA基因在工程菌中的表达,最后还对工程菌转化柔红霉素生成阿霉素的发酵工艺进行了初步研究。

ABSTRACT The final production of erythromycin by Saccharopolyspora erythraea was increased by 43.1% in 15L bioreactor with double quantily of soybean oil into culture medium. The final production, production capacity and Yp/s of erythromycin were improved 69.7%, 69.6% and 63.8%, respectively, in 15L bioreactor with added soybean oil during fermentation.

红色糖多孢菌D在15L发酵罐的基础培养基和发酵过程中补加豆油,当基础培养基中豆油量加倍时,效价提高43.1%;发酵过程补加豆油罐的红霉素效价、生产强度和Yp/s比不补豆油的分别提高了69.7%、69.6%和63.8%。

ABSTRACT The final production of erythromycin by Saccharopolyspora erythraea was increased by 43.1% in 15L bioreactor with double quantily of soybean oil into culture medium.

作者:沈兆兵陈国豪陈长华红色糖多孢菌D在15L发酵罐的基础培养基和发酵过程中补加豆油,当基础培养基中豆油量加倍时,效价提高43.1%;发酵过程补加豆油罐的红霉素效价

The inhibitory rate of metabolites produced by TD103-UV-4 against RS was more than 80%, and the antibiotic production capacity increased by 47.1%, meanwhile, its metabolites had a strong inhibition against other phytopathogenic fungi, such as Botrytis cinerea.

其中菌株TD103-UV-4发酵液对立枯丝核菌的抑制率在80%以上,并且对灰葡萄孢菌等植物病原菌有较强的抑制作用,产抗生素能力比出发菌株提高了47.1%。

Result The phytopathogenic fungus Pythium aphanidermatum was isolated; 7 strains of antagonistic actinomycete, which had obvious inhibitory effects on Pythium aphanidermatum were screened. The inhibitory ring of No.8 strain was 13.3 mm, and reached 88.3% when cultivated for 120 h.

伽师甜瓜猝倒病的病原菌为瓜果腐霉;筛选出7株对瓜果腐霉具有显著拮抗性的生防放线菌,其中8号放线菌对瓜果腐霉的拮抗环宽度为13.3mm,其无菌发酵滤液对瓜果腐霉的抑菌率在培养120h时高达88.3%。

Using Sarcina lutea as original strain,a ethionine and zinc chloride-resistant mutant was screened after treated with UV.

通过对谷胱甘肽新产生菌———藤黄八叠球菌进行紫外诱变处理,筛选到一株抗乙硫氨酸和氯化锌的抗性菌株,该突变株发酵生产谷胱甘肽的产量比出发菌株提高268.9%。

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