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The results indicate that different molecular weight of dextran could be obtained by direct fermentation,and the acid hydrolyzing in the old technology be avoided.The yield reached the maximum by controlling the pH value to be 8.0,and the inorganic salt could promote the growth of the Leuconostoc mesenteriodes with the effect of promotion being Mn2++Ca2+>Mn2+>Ca2+.

结果表明:通过定向发酵技术可得到符合要求的不同分子量的右旋糖酐,避免了老工艺中酸水解工艺;控制发酵培养基pH值为8.0,可使产率最大;无机盐离子Mn2+和Ca2+能促进L.M菌的生长,其促进菌生长的程度大小依次为:Mn2++Ca2+>Mn2+>Ca2+。

Among those results,the efficiency of leuconostoc mesentervides was the best one; and the nitrite peak was inhibited clearly by adding garlic juice or ginger juice during fermentation.

结果表明:采用纯接种技术,在发酵前期可有效降低亚硝酸盐的含量,其中以接种肠膜明串珠菌效果最佳;同时在发酵过程中添加适量蒜汁或姜汁,可明显地抑制甘蓝乳酸发酵中亚硝峰出现;且在发酵过程中,泡菜的pH值对亚硝酸盐含量也有较大的影响。

Fed-batch fermentations of vitamin B12 by Propionibacterium shermanii were conducted in a 7L fermentor to investigate characteristics of the cell growth and vitamin B12 production.

首先对维生素B12产生菌Propionibacterium shermanii在7L发酵罐内分批发酵下的菌体生长及产维生素B12的特性进行了研究。

In these studies, 2 microbes were adopted. The time of fermenting CBM by DT and JM funguses involved respectively 3 stages: 5 days, 10 days and 15 days. After the trial of fermentation, the content of ricinine and allergen was detected.

本研究所采用的微生物为DT菌和JM菌,其发酵处理蓖麻饼的时间均分别为5d、10d、15d,发酵后测定并比较了它们对蓖麻碱、变应原的去除率及蓖麻饼中营养成分含量的变化。

With routine method, from having elected contents higher one RNA bacterial strain among several saccharomycetes, beer yeast, and the method with Shan factor experiment have studied an experiment being bacterial strain's turn to produce more proper RNA culture medium made up of , optimum the pH value fermenting and the best fermentation time.

本实验用常规方法从数株酵母菌中选出了一株RNA含量较高的菌株——啤酒酵母,并用单因素实验的方法研究了该菌株产RNA较适宜的培养基组成、最佳发酵pH值和最佳发酵时间。

It showed that the growth of the strain was positive correlated to the flocculating activity at different cultivation time.

通过研究该菌株在不同培养时间的生长情况和发酵液的絮凝活性,从而得出发酵液絮凝活性与菌体生长量呈正相关。

This achievement is mainly about producing natural astaxanthin by fermentation method. Techniques including strains isolation, breeding astaxanthin overproducing strains, optimization of fermentation process and astaxanthin extract process.

本成果利用发酵法生产天然虾青素,对虾青素产生菌株的分离、高产菌株的选育、虾青素发酵工艺的优选、虾青素提取技术的优化等进行系统地研究。

Furthermore, the effects of different metallic salts,nitrogen sources,carbon sources,temperature,pH,water content and fermentation time on the enzyme activity and the yield of the acid protease were studied and the results were as follows: both MnCl2 and CuSO4 could activate the protease while other metallic salts inhibited the protease on different levels; addition of 1 % bean flour and 1 % glucose could increase enzyme yield and enzyme activity by 71 % and 31 % respectively; the maximal yield of enzyme was achieved under the conditions of temperature at 40 ℃ and pH as 7.0; water content and fermentation time would also influence enzyme yield on different levels.

不同金属盐对该酶酶活及不同氮源、碳源、温度、pH、含水量以及发酵时间对菌株产酶的影响试验结果表明:MnCl2,CuSO4均对该酶有激活作用,其他金属盐类对该酶有不同程度的抑制作用;添加黄豆粉(1 %)和葡萄糖(1 %)可使菌株产酶酶活分别提高71 %和31 %;培养温度为40 ℃以及培养基起始pH为7.0时产酶最高;含水量和发酵时间对产酶也有不同程度的影响。

The optimum condition for separating and purifying SAM was using the solution at pH of 5.0 containing 3.0 g SAM per liter at the flow velocity of 6 L/h through the ion-exchange column (φ50 mm×1500 mm)filled with D152 resin. Impurities were removed first by eluting the column with double-distilled water and acetic acid, and then with 9 L to 15 L of sulfuric acid of 50 mmol/L concentration at the flow velocity of 6 L/h.

论文对SAM的性质、药理作用以及当前国际上关于SAM的研究进展进行了较为详尽的综述,并在此基础上确立了研究内容,取得了如下成果: 1利用本实验室构建的基因工程菌JM71发酵生产S-腺苷-L-蛋氨酸,在50 L发酵罐中发酵6天后,SAM终产量达到6 g/L发酵液。

The quality of purified 2-KGA from its fermentation products was up to industrial standard. In addition, several hexoses, such as D-glucose and D-sorbitol can be utilized to synthesize 2-KGA by this newly-mixed culture. The growth and anabolism of the B529-V6 were studied in 4M〓 and 300M〓 fermentor respectively. The results showed that its growth and anabolism were affected by carbon sources, ratio of D-glucose and L-sorbose, nitrogen sources, growth factors, inoculum quality and culture condition including the initial pH value, temperature and ventilation.

新菌系B529-V6表现出了较强的高浓度L-山梨糖耐受能力、较高的底物代谢速率和较高的2-KGA转化能力,在8%山梨糖浓度的发酵培养基中培养48h,糖酸转化率较对照菌系提高了5.94%;山梨糖浓度提高至10%,其生长代谢受影响程度较小,且能不同程度地利用葡萄糖和山梨醇为底物,合成维生素C前体-2-酮基-L-古龙酸(2-KGA),其发酵产物2-KGA经反相高效液相色谱分析其质量符合工业化生产要求。

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