发酵菌

More metabolites could be detected when the TLC was carried out in system II (chloroform∶methanol=9∶1) and system III (butanol∶acetic acid∶water=4∶1∶5) with anisaldehyde test solution.

对每一株菌而言，产生最多产物的发酵培养基各不相同，为了获得尽可能多的次生代谢产物，不同菌株选用的发酵培养基不一样。

The strains were selected with liquid fermentation method and the strain was identified through the observation of the growth of the samples cultured for 18, 48 and 72 hours under the treatment of smear stained with crystal violet dye.

采用液体发酵的方法筛选降解苄嘧磺隆的菌株，分别于18、48、72 h取发酵液，用结晶紫染液涂片染色，观察细菌的生长情况，对涂片结果中菌体生长较好的菌种进行菌种鉴定。

And the last, the effects of fermented cottonseed-hull on dairy performance were studied with feed tests. In experiment 1, Cottonseed-hull and comminuted cottonseed-hull were fermented with complexing strains of microbials including EM and probiotics, using seven pairs of formulation have been formed by corn meal, molasses and fecula combined with urea and zein meal.

试验一利用EM菌液与益生素混合扩大培养后的复合菌液，选用玉米粉、糖蜜、淀粉三种炭源和尿素、玉米蛋白粉两种氮源共组成了7组发酵底物配方，将棉籽壳分成微粉样与原始样，发酵结束后对测定结果进行统计分析。

An astaxanthin overproducing strain was obtained by mutagenesis. in 200l common stirring fomenter, 60mg/l - 70mg/l astaxanthin were harvested by fed-batch cultivation of phaffia rhodozyma. results of optimization of extract condition indicated astaxanthin productivity was 1.14mg/g by acid methods under optimal condition. this achievement reached to the leading level of the field in china, and also advanced method internationally. in the same time, it is a good basis for the further research for the fermentation of natural astaxanthin.

分离选育出虾青素高产的海洋红酵母，在200l中试罐中发酵虾青素，虾青素的产率达60-75mg/l发酵液；对天然虾青素的无毒提取技术进行研究，虾青素的提取得率为1.14 mg/g干菌体；虾青素发酵水平和提取技术水平为国际先进，国内领先，为用发酵法生产天然虾青素打下坚实的基础。

An astaxanthin overproducing strain was obtained by mutagenesis.In 200L common stirring fomenter, 60mg/L - 70mg/L astaxanthin were harvested by fed-batch cultivation of Phaffia rhodozyma. Results of optimization of extract condition indicated astaxanthin productivity was 1.14mg/g by acid methods under optimal condition. This achievement reached to the leadinglevel of the field in China, and also advanced method internationally. In the same time, it is a good basis for the further research for the fermentation of natural astaxanthin.

分离选育出虾青素高产的海洋红酵母，在200L中试罐中发酵虾青素，虾青素的产率达60-75mg/L发酵液；对天然虾青素的无毒提取技术进行研究，虾青素的提取得率为1.14 mg/g干菌体；虾青素发酵水平和提取技术水平为国际先进，国内领先，为用发酵法生产天然虾青素打下坚实的基础。

The objective of the study was to find out the effect of Lactobacillus flora on the quality of yoghurt.

本试验从乳酸菌的分离开始，进行了单菌发酵，混合发酵，来比较研究乳酸菌菌相对酸乳品质的影响。

First,126 samples were gathered in the natural environment in which grew to the lactobacillus .

首先在各种适合于乳酸菌生长的自然环境中采集了126个样品，然后进行了乳酸菌的筛选试验，优选出2株L-乳酸产量较高且遗传性比较稳定的菌株，对该两株菌的种子培养条件及影响L-乳酸发酵的因素进行了系统的研究，同时对玉米芯L-乳酸发酵进行了初步的探讨。

Based on that the dielectric permittivity of cell suspensions is monotonic function of the measuring frequency and cell concentration at radio-frequencies,a new method for detecting cell concentrations on-line and in-situ is proposed .

利用发酵液在无线电频率范围内的电容率是测量频率与菌体浓度的函数，提出了一种实时在线检测发酵过程菌体浓度的新方法，设计了检测传感器和自动检测系统，并且实验验证了该检测方法及检测系统的可行

This fermentation system has some advantages, such as long-Cycle, which make for the synthesize of officinal ingredients.

亮菌液体发酵工艺研究结果：采用"复杂系统调控技术"确定亮菌发酵的最适因素及其相应量值。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。