发酵罐

Moreover by orthogonal optimization the culture condition and the culture medium were investigated,then the result of optimization was used to fermentor.

另外利用正交法对spMF0507的培养基组成和发酵培养条件也进行了研究，之后把发酵优化结果投入5升发酵罐进行验证。

Based on the above optimum condition, the batch fermentation was performed with strain SW0370 in 7-liter fermentor.

以摇瓶分批发酵最优条件为基础，对菌株SW0370进行了7L发酵罐分批发酵试验。

Through solid fermentation process, the mixture of sodium humate, oilcake and rice husk are mixed with bacterial liquid and inorganic elemnt solution, the final mixture is formed to various shape and fermented in solid fermentation cylinder for 2-5 days before drying, natural drying and crushing.

采用固体发酵法，将腐植酸钠、油料饼粕和谷壳粉混合，按料液比1∶0.5～1.3，加入10～30％的菌液和40～100％的无机元素溶液，搅拌均匀后制成各种形状，再装入固体发酵罐发酵2～5天，干燥，自然冷却，粉碎。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化，重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达，菌体密度最终达到115g/L以上，可溶性重组融合蛋白占菌体总蛋白的30%以上，含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养，结果表明该工艺能有效的放大，可适用于工业生产。

ABSTRACT The final production of erythromycin by Saccharopolyspora erythraea was increased by 43.1% in 15L bioreactor with double quantily of soybean oil into culture medium. The final production, production capacity and Yp/s of erythromycin were improved 69.7%, 69.6% and 63.8%, respectively, in 15L bioreactor with added soybean oil during fermentation.

红色糖多孢菌D在15L发酵罐的基础培养基和发酵过程中补加豆油，当基础培养基中豆油量加倍时，效价提高43.1%；发酵过程补加豆油罐的红霉素效价、生产强度和Yp/s比不补豆油的分别提高了69.7%、69.6%和63.8%。

ABSTRACT The final production of erythromycin by Saccharopolyspora erythraea was increased by 43.1% in 15L bioreactor with double quantily of soybean oil into culture medium.

作者：沈兆兵陈国豪陈长华红色糖多孢菌D在15L发酵罐的基础培养基和发酵过程中补加豆油，当基础培养基中豆油量加倍时，效价提高43.1%；发酵过程补加豆油罐的红霉素效价

Each of three lactic acid bacteria from EZAL-MY96 was cultured and fermental techniques were studied by experiments with 5L fermentive vessel. Technique parameters of three lactic acid bacteria were determined.

对EZAL-MY96直投式酸奶发酵剂中分离出来的3株乳酸菌利用5L发酵罐进行发酵工艺研究，确定3株菌中每一菌株的最佳发酵工艺参数。

In order to reach a higher productivity level of mycelium and exopolysaccharide ,L934 orthogonal experiments on fermentation with Schizophyllum commune were carried out in 7 L fermentor.

在7 L发酵罐上进行了裂褶菌发酵生产菌丝体和胞外多糖的研究，采用L_9(3~4)正交试验对发酵条件进行了优化。

Schizophyllum commune is a kind of edible and medicinal fungi,which contains different kinds of bio—active constituents,such as polysaccharides.

在7 L发酵罐上进行了裂褶菌发酵生产菌丝体和胞外多糖的研究，采用L_9(3~4)正交试验对发酵条件进行了优化。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。