发酵的

The recombinants with different multiple copies of amyL integrated in the chromosome were identified by real-time PCR and evaluated by shake-flask fermentation.

对转化子的amyL拷贝数及其BLA发酵水平分别用荧光实时定量PCR及摇瓶发酵试验进行评价与鉴定。

It focuses on the anabolism pathway ,screening of strain and fermentation conditions.

对发酵法生产D-核糖的生物合成途径、菌种选育、发酵条件等进行了综述。

The application areas includes the improvement of new-type China liquor by pervaporation, the production of apple brandy and apple acidic beverage by fermentation of apple juice with a PDMS membrane bioreactor and the production of fuel ethanol by continuous fermentation in a silicone rubber membrane bioreactor.

本论文对硅橡胶复合膜结构及其渗透蒸发性能展开了较为系统的研究，同时进行了PDMS膜用于渗透蒸发过程对白酒风味成分重整、苹果原汁发酵制造白兰地和酸性饮料产品、连续发酵生产燃料乙醇三方面的应用研究。

Wastewater containing titanium pigment was treated by microfiltration membrane, Nanometer titanium can be purified and concentrated by use of microfiltration. Operational conditions were optimized and the model was built. Finally, the amount of wash water was reduced. Based on the above research, the whole set equipment was designed, which can meet the needs in a titanium industry with a capability of 500 ton per year. Backflush and automatic control were used in the equipment, which has been steadily run two years, has been widely adopted in titanium industry. The technology has been widely adopted in titanium industry.In the recent years, whole set equipment of ceramic membrane was increasingly used in the recovery of desired production from fermentation broth in China. A new membrane process integrated with pretreatment of flocculation or coagulation was exploited to recover Inosine. ZrO2 ceramic membrane was used in the work, and the average permeation flux could reach 150L/m2.h under pH value of 3, transmembrane pressure 0.1MPa, CFV 3m/s, and temperature higher than 70C. The recovery ratio of inosine is higher than traditional recovery method, although inosine degraded under acidic and high temperature condition.

实验表明，ZrO_2膜处理肌苷发酵液的合适条件为pH=3、操作压力为0.1MPa、膜面流速3m／s、温度大于70度，此时可以获得平均渗透通量150L／m~2h，但由于酸性条件、高温下肌苷降解，导致肌苷收率有所降低，但仍高于传统肌苷提取方法得到的收率；为进一步提高肌苷得率，缩短提取工序，实验研究了絮凝剂预处理发酵液，解决碱性条件下由于蛋白析出导致膜污染加重的问题，研究表明采用CaCl_2絮凝并调节pH=11，可以获得平均渗透通量为180L／m~2h，肌苷得率大于95％；比较了三种肌苷提取工艺得到的产品质量，采用预处理与陶瓷南京工业大学博士学位论文膜分离结合的除杂工艺得到的粗昔质量与陶瓷膜分离、离子交换及活性炭吸附工艺生产的粗普质量相当，均优于传统的提取工艺；设计了每小时处理12一巧吨肌普发酵液的陶瓷膜成套装置，采用部分内循环的方式降低设备的能耗，简化了肌昔提取方式，提高了产品的收率和得率，由于减少离子交换和活性炭吸附，使得工厂废水排放量降低30一40%，降低酸、碱用量，有利于环境保护。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

In order to cutting down the cost, flocculation of recombined Erwinia carotovora-Eccl3B cell from broth, influence of temperature, pH and oxidants on the separation of PHB were studied; and the method which measure the molecular weight of PHB by intrinsic viscosity was also studied; furthermore, a phenomenon was found that insolubility of PHB in chloroform can be changed by pretreated at over 120℃ for the fist time, and the mechanism of this phenomenon was explored.

为了降低成本，提高生产效率，本文针对发酵法生产PHB过程中存在的，发酵液难以分离沉淀，非有机溶剂提取PHB时分子量严重降低等问题，进行了发酵液的絮凝，及温度、pH、氧化剂对提取PHB影响的研究。并对粘度法测定PHB分子量的方法进行了研究，同时，对于研究过程中发现的，加热后PHB在三氯甲烷中的溶解性变化及其原因进行了探索。

To culture antibiotic overproducing strains, the study include using different physical and chemical factors to induce Streptomyces thermocarbonxydus var shandaensis to mutation. After original strain were mutagensised by UV combined LiCl、~(60)Co、 DES, 9 high-yield strains were obtained.

通过紫外线加氯化锂复合诱变得到高产菌株UV—18、UV—19、UV—20，其中菌株UV—19的发酵上清液抑菌圈直径是原种的1.875倍；通过~(60)Co诱变得到高产菌株Co—22、Co—36、Co—38，其中菌株Co—38的发酵上清液抑菌圈直径是原种的1.68倍；通过硫酸二乙酯诱变得到高产菌株DES—21、DES—31、DES—37，其中菌株DES—21的发酵上清液是原种的1.52倍。

A song and yeast all imported from Japan, the wine and yeast production projects, strictly control the ratio of raw materials and production and health to prevent the intrusion of the Harmful microorganisms, strictly control the fermentation process Fermentation temperature, close monitoring of the low temperature sterilization, reducing the wine of nutrients in the destruction of the production cycle for two months, the more rich aroma of wine, I feel more alcohol and.

种曲和酵母全部从日本进口，酒母及发酵生产工程中严格控制各种原料的比例和生产卫生，最大限度的阻止有害微生物的侵入，发酵过程中严格控制发酵温度，密切监测，灭菌的温度低，减少了酒中的营养物质的破坏，整个生产周期长达两个月，使酒的香气更加浓郁，口感更加醇和。

This product has effects on promoting wine yeast to reproduce and increasing yeast population.So it could ferment red and white wine to full dryness,and accelerate sluggish wine to ferment again.The finished wine had better quality than

摘 要：研制了一种新型酵母活性剂，具有促进酵母繁殖、增加酵母群体数量的作用，从而使红、白葡萄酒的发酵更为完全、彻底，并能使发酵出现停滞的葡萄酒再次触发发酵；所生成的葡萄酒感官与理化指标均优于对照样，达到优质葡萄酒的标准。

The results indicated that the regulation of the acidity of raw juice could prevent bacteria contamination and prolong 2～3 d shelf period under homothermal condition (temperature at 29 ℃), the addition of active dry yeast with high temperature tolerance (addition level as 0.1g/100 mL) could realize thick mash fermentation, the fermentation time should be controlled about 28 h, and sugar content of fermenting mash was 20.6 g/100 mL and the alcohol content of matured mash reached 12.8 %vol.

结果表明，调整原汁的酸度，能够防止杂菌污染，在29 ℃恒温条件下，可延长甜高粱榨汁的保存期至2～3 d；耐高温活性干酵母的加量为0.1 g/100 mL，并可实现甜高粱秸秆榨汁的浓醪发酵；发酵时间控制在28 h左右；高糖度发酵醪含糖量为20.6 g／100 mL，成熟醪酒精度达12.8 %vol。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。