发酵的

In order to achieve a high concentration and high productivity of exopolysaccharide from Auricularia auricula, the effect of pH on the performance of EPS production in submerged fermentation was investigated. It was showed that the maximum yield of EPS （7.54 g/L） was obtained at pH 5.5, but the mycelial growth rate was enhanced at pH 5.0 and the maximum dry cell weight was

为了实现黑木耳深层发酵生产胞外多糖的高产量和高生产强度的统一，在7L发酵罐中研究不同pH对A.auricula分批发酵生产黑木耳多糖的影响。A.auricula细胞生长的最适pH为5.0，而黑木耳胞外多糖合成的最适pH为5.5。

Besides, compounding bacteria was preferred for highland barley wine production by liquid fermentation with its addition level 0.4 %～0.6 % of the total weight of highland barley powder, fermentation time between 40～72 h, and water addition level 4～6 times of the volume of highland barley powder.

结果表明，优化发酵工艺条件可提高青稞酒的出酒率，对酒的色泽、口感等感官质量无影响；复合菌种适宜于液态发酵生产青稞酒，添加量为青稞粉重的0.4 %～0.6 %；发酵时间为40～72 h；加水量为青稞粉的4～6倍。

Chapter 2 argues the present status of parameter measurement in fermentation, including the methods and equipment, and the restrictions fermenter applies to them.

在第二章中，本文对目前微生物发酵过程参数的检测状况进行了全面的介绍，主要包括对发酵过程中溶解氧和其它各参数进行测量的常用方法和仪表，以及发酵过程对这些方法和仪表的特殊要求。

According to the characteristic of fermenter, we designed the control system based on single chip computer and personal computer, realizing the measurement and control over DO, pH, temperature, pressure, ventilating rate and the speed of the stirring motor.

根据发酵罐的特点，本文基于单片机和PC机结合，设计了发酵过程自动控制系统，实现DO、pH、温度、罐压、通气量和搅拌电机转速的测控以及发酵液的粘度的在线测量。

In 50L fermentor, the metabolic characteristics of carbohydrate, DO, NH〓-N and dissolved phosphorus, cell growth and CTC biosynthesis were analyzed intensively. It was put forward that the cell growth on the initial stage of fermentation must be controlled properly in order to achieve the goal of fermentation process optimization.

研究了50升发酵罐中CTC发酵过程的糖、氧、氮和磷的代谢特性以及菌体生长和金霉素合成的规律，并提出通过在发酵前期控制菌体生长的方法来达到过程调控和优化的目的。

On the basis of Saccharification of the liquid component analysis and processing of corn stalk remnants of the scanning electron microscope on the surface of the micro-structure .

在对糖化液成分分析及预处理后的玉米秸秆进行了扫瞄电镜的表面结构的微观分析基础上，进行了玉米秸秆糖化液的发酵前预处理和秸秆糖化液的发酵条件，以及构建双菌株发酵体系进行了研究。

With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.

通过对培养条件的不断优化，重组葡激酶-水蛭素融合蛋白在大肠杆菌BL21(DE3)里得到了高效表达，菌体密度最终达到115g/L以上，可溶性重组融合蛋白占菌体总蛋白的30%以上，含量约为1.1~1.2g/L.5L发酵罐的发酵工艺参数在40L发酵罐中进行了放大培养，结果表明该工艺能有效的放大，可适用于工业生产。

The fermentation condition of Acetobacter xylinuml.1812 has been optimized by single factor and orthogonal experiments including the components of medium and the initial fermentation condition and the fermentation parameters.

通过单因素和正交试验，优化了木醋杆菌产细菌纤维素的发酵条件，包括培养基成份的优化、初始发酵条件的优化和发酵动力学参数的测定。

This paper focused on the breeding of an L-lactic acid producing bacteria Lactobacillus easei YBQ1-1, studying the characteristics, medium components, pH and metabolic network of the mutant YBQH2-14 obtained after being selected.Using UV irradiation, L.casei YBQH2-14 was selected in the different plate media such as the medium with high concentration of glucose, lactic acid , CaCO3 -Bromocresol purple and KMnO4 -KBr.

本文主要对L-乳酸生产菌株(Lactobacillus casei YBQ1-1)进行了诱变选育，并研究了突变株YBQH2-14发酵条件对生产L-乳酸的影响，构建了L.caseiYBQH2-14的代谢网络模型，分析了分批发酵不同阶段的代谢通量，同时研究了L-乳酸的应用——黑麦麸皮粉发酵。

Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群，序列同源性大于99%，与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6，6~18，18~66和66 h；培养12 h后，YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56，此后逐渐上升，至发酵结束时其pH值分别为7.74和8.07；培养0~18 h时2菌株发酵液中还原糖含量迅速降低，此后保持稳定；12 h时氨基氮含量达到最低，此后开始缓慢上升；YL001菌株培养54 h后及YL002菌株培养42和66 h后，其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。