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The mutants were further screened through repeated fermentations as well as the measurement of their physical properties. The results showed that some major fermentation indexes of strain HJ1615-d4 such as alcoholicity, fermenting power,total acids and flavoring components etc. were almost the same as those of the original strain. However, its urea yield was only 15.41 mg/L, louer 50 % than that of the original strain.

对这4株突变株进行发酵栓实验,并测定其发酵综合性能,其中菌株HJ1615-d4在发酵力、总酸、酒精度以及风味物质等综合指标方面保持了亲株的优良性状,同时产尿素的量为15.41 mg/L,低于出发菌株产尿素量50 %以上。

Three developing systems were used for chromatography of TLC. UV 254nm, 366nm and anisaldehyde as a chromogenic agent were used for detecting compounds of these strains.

本研究设计4种内生菌发酵培养基,对89株分离自傣药植物且具有高抗菌活性的内生菌及5株潜在放线菌新种进行摇瓶发酵,发酵液的提取物用3种展层系统作薄层色谱展层,用UV(254nm、366nm)和茴香醛试剂进行检测。

The experiment optimized the culture medium and conditions for PV 1 to yield the highest GABA: sucrose as carbon source,peptone and beef extract as complex nitrogen source,initial pH at 7.0,temperature at 30~34 ℃.The content of GABA was over 4 g/L in the optimum culture conditions.Thereby,i...

实验还对酸菜1号菌株的培养基和培养条件进行了优化:用蔗糖为碳源、蛋白胨和牛肉膏作为复合氮源、培养基初始pH为6.0、培养温度为30~34℃时该菌株发酵产GABA的量最多,发酵过程中的振荡培养(100 r/min)和静置培养对GABA的产量无明显影响;在最佳培养基组合和发酵条件下,发酵液中GABA含量可达4 g/L以上,表明利用乳酸菌进行富含GABA食品的生产是可行的。

The optimal conditions of production for red-jujube and honey health-care wine using the methods of soak-prepare and rice brewage were investigated in this article.

采用浸提调配法和纯粮酿造法制备红枣蜂蜜保健酒,对2种工艺进行研究,得到红枣最适热烫时间5min,发酵前糖度10%~15%,红枣取汁时红枣果肉与水的质量比为1:8,采用南阳酵母作为发酵菌株,接种量为3%~5%,发酵6d,可获得高质量的红枣蜂蜜保健酒。

An astaxanthin overproducing strain was obtained by mutagenesis.In 200L common stirring fomenter, 60mg/L - 70mg/L astaxanthin were harvested by fed-batch cultivation of Phaffia rhodozyma. Results of optimization of extract condition indicated astaxanthin productivity was 1.14mg/g by acid methods under optimal condition. This achievement reached to the leadinglevel of the field in China, and also advanced method internationally. In the same time, it is a good basis for the further research for the fermentation of natural astaxanthin.

分离选育出虾青素高产的海洋红酵母,在200L中试罐中发酵虾青素,虾青素的产率达60-75mg/L发酵液;对天然虾青素的无毒提取技术进行研究,虾青素的提取得率为1.14 mg/g干菌体;虾青素发酵水平和提取技术水平为国际先进,国内领先,为用发酵法生产天然虾青素打下坚实的基础。

The results showed that the bacteria B7 came into the logarithmic growth phase for 5 h culture. Both the fermentation liquid and its filtration could inhibit C. colhounii effectively, and the fermentation of dilution l0 times also showed significant differences compared with the control. The inhibiting effect of its fermentation media filtration was even more significant in the optimizing test, with the maximal inhibition rate of 100%. The best fermentation condition was 0.5% glucose, 0.1% ammonium muriate, without extra mineral, pH 5.4, temperature 28℃. Based on the morphological properties and 16S rRNA sequence, the bacterium B7 was identified as the Bacillus subtilis.

结果表明,该菌体在培养了5h后即进入对数生长期;B7细菌的发酵液原液和无菌滤液均能抑制病原菌的生长,其中发酵液原液的10^3稀释液的抑菌效果仍与对照之间存在显著的差异;发酵条件优化试验中发现该细菌的无菌滤液最大抑菌率达100%,最佳发酵条件为葡萄糖0.5%、氯化钱0.1%、不加无机盐、pH5.4,温度28℃;结合形态学特征和16S rRNA的序列分析,将该菌株鉴定为枯草芽孢杆菌。

Anaerobic synecology of the hydrogen-producing fermentation system was investigated under certain test condition in this thesis. In the continuous flow system of anaerobic hydrogen-producing fermentation, the succession of hydrogen-producing acidogenic microbiology may be regressive process. By regressive process we means that the original community getting from nature which structure being complex with a high ability would degenerate gently to a certain climax community which structure being simple with less ability.

产氢发酵系统的微生物群落生态学研究证明,在连续流发酵产氢反应系统中产氢产酸发酵细菌群落的演替过程,是在人为控制的生境条件下,微生物从自然群落这种稳定性大、组成及生化反应过程复杂的生态群落,向稳定性小、组成和生化反应过程相对简单的某一产酸发酵顶极群落进行的"逆行演替"。

Shandong Shengli Bioengineering Co., Ltd., Jining 272037, Shandong, China Abstract: The cause of infection in the process of avermectin production was studied from infected time, scope and bacterial type. The test method to check the infection caused by other bacteria was also raised. The ways to prevent infection was ascertained from preparation of microbial strain, air purification, medium disinfection, ductwork deployment and systematic dead angle cleaning. Meanwhile, adjustment measure was summarized in different infected period such as microbial strain, seed pot and yeastiness.

山东胜利生物工程有限公司,山东济宁 272073 摘要:从染菌的时间、类型、范围三方面探讨了阿维菌素发酵染菌的原因,提出了发酵过程中杂菌的检查方法,分别从菌种制备、空气净化、培养基灭菌、管路配置、系统死角清理等方面明确了防止发酵染菌的措施,得出了斜面菌种、种子罐、发酵罐不同时期染菌后的补救措施。

The process and condition in the preparation of dextran through the fermentation of Leuconostoc mesenteriodes 0326 were discussed in the paper.The adhesive viscosity,pH value, the production of fructose and dextran,variation of molecular weight and the fermentation inducer were investigated,and the related curves of the dextran fermentation presented.

文章对由肠膜状明串珠菌L.M0326发酵生产右旋糖酐的工艺过程及条件进行了探讨,通过对其发酵过程中的粘度、pH值、果糖、右旋糖酐生成和分子量变化的研究及对发酵诱导物的考察,得到了右旋糖酐发酵工艺的相关工程曲线。

In the paper,the fresh,withered,rocked and fermented leaf fixed by microwave and steam were studied.The results showed that the content of pol yphenols of microwave samples was higher than those of steam samples;and the con tent of amino acids in steam samples was higher than those of microwave samples .There was a slight higher content of soluble sugars in microwave samples compa red with steam samples. The content of chlorophyll of microwave samples was hig her than steaming samples,and there was a small difference of caffeine content b etween microwave and steam samples.There was a little higher content of theafla vins in microwave samples than steam samples.The content of thearubigins in steam samples was higher than microwave samples,and there was no differe nt theabrown between microwave and steam samples.

摘 要:对鲜叶、萎凋叶、做青叶和发酵叶进行了微波和蒸青固样研究,结果表明:微波样的多酚类化合物保留量均高于蒸青样,氨基酸保留量则相反,可溶性糖的保留量微波样稍高于蒸青样,叶绿素的保留量微波样亦稍高于蒸青样,咖啡碱的保留量微波样与蒸青样间差异较小,发酵叶茶黄素的保留量微波样稍高于蒸青样,发酵叶茶红素的保留量微波样高于蒸青样,发酵叶茶褐的保留量二者间无差异。

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