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The medium composition and some fermentation parameters were studied by cross test and single factor experiments. The optimal conditions are as flows: Tryptone 0.76 %, Bean powder 1.0 %,initial pH 8.0, temperature 28 ℃, inoculation quantity 8 % and loading volume 30 %. Under optimal conditions, the maximum yield of biomass and microbial lipids reached 29.8 g/L and 11.7 g/L, respectively, which was 2.59 times and 2.05 times of those cultured in the original medium, after 9 days fed-batch co-fermentation in shake-flask.

采用单因子和正交实验对培养基组成和发酵条件进行了优化,结果表明,诱变菌在温度28 ℃,pH 8.0,接种量8 %,装液量30 %,蛋白胨0.76 %和豆粕1 %,分批补糖的最优发酵条件下发酵9 d,生物量和菌体油脂积累量分别达29.8 g/L和11.7 g/L,较出发菌提高了2.59倍和2.05倍,同时原料糖利用率达到99.4 %。

The experimental results suggested that there were some rules during the fermentation as follows: pit temperature increased rapidly and then dropped at the beginning of the fermentation and kept stable eventually at 20~22 ℃; there was slight change in yellow water pH value at first and then pH value decreased gradually in middle fermentation stage and finally kept stable; the change rules of total acids of yellow water were opposite to pH change rules; the reducing sugar of yellow water increased at first and then dropped and kept stable eventually; there was almost no change in alcohol content of yellow water in prior fermentation period, and then it increased rapidly and dropped slowly and finally kept stable; gas production rate was as high as 550 L/d within the first week in fermentation and then dropped undulatingly and changed to zero or even negative gas production rate.

窖池温度在发酵前几天迅速升高,然后下降,最后趋于稳定,保持在20~22 ℃;黄水pH开始变化不大,中间逐渐下降,最后趋于稳定;黄水的总酸变化与pH相反;黄水中还原糖先升高后下降,最后基本保持稳定;黄水中酒精含量在发酵前期变化不大,后期先是迅速升高,然后缓慢下降,最后趋于稳定;在发酵前期一周内,产气量很大,最高达550 L/d;之后产气量呈波动式下降并逐渐平稳,最后趋于零甚至负产气量。

The culture filtrate of strain LN-176 was tested for light absorption at 260nm and 280nm, and the results showed that the protein content in the culture filtrate significantly increased. After ammonium sulphate precipitation, the supernatant and the precipitation were separated to testify their antagonistic activity, and it was found that the precipitation was inhibitory to Ustilago hordeum , but the supernatant not.

LN-176菌株的发酵液在260nm和280nm下进行吸光度测定,结果表明发酵液中蛋白质含量明显升高;发酵液经硫酸铵盐析后,取上清液和沉淀物分别做抗菌试验,发现沉淀物对青稞散黑穗病菌有拮抗活性而上清液无活性。

In This paper .thirty strains of yeast were isolated from nineteen samples of fermented milk(10 koumiss samples.three fermented goat milk samples.four fermented milk samples,two shubat samples) that were collected from the west-centre region of Inner Mongolia from which we aimed to select the yeast with high vitalistic b-Galactosidase .

本研究主要以内蒙古中西部地区牧区采集的19个发酵乳样品(10份酸马奶、3份发酵山羊奶、4份酸牛奶和2份发酵驼奶)为研究对象,分离获得30株酵母菌,从中筛选乳糖酶高活力酵母菌株。

In This paper ,thirty strains of yeast were isolated from nineteen samples of fermented milk(ten koumiss samples,three fermented goat milk samples,four fermented milk samples,two shubat samples) which were collected from the west-centre region of Inner Mongolia from which we aimed to select the yeast with high vitalistic β—Galactosidase .

本研究主要以内蒙古中西部地区牧区采集的19个发酵乳样品(10份酸马奶、3份发酵山羊奶、4份酸牛奶和2份发酵驼奶)为研究对象,分离获得30株酵母菌,从中筛选乳糖酶高活力酵母菌株。

The advantages of the simple twice zymotechnics lie in the characters of the cultivation materials, the growth of mycelia, the control of plant diseases and insect pests, the output of Volvariella Volvacea and the production cost. All these indicate that simple twice zymotechnics is a relatively reasonable compost treatment in Volvariella Volvacea cultivation.

简易二次发酵法的栽培材料理化性状、草菇菌丝生长、病虫害控制和草菇的产量、生产成本等,都比自然堆沤发酵法和常规二次发酵法有较大的优势,表明简易二次发酵法是草菇栽培中较理想的一种培养基处理方法。

Vinification: After 12-days of maceration, the must is decanted in stainless steel tanks, in which it takes place the malolactic fermentation.

发酵方式:经过12天的发酵后,导入不锈钢罐中进行苹果酸-乳酸发酵。

The cold winter temperatures put a temporary stop to the fermentation process, but it starts to ferment again in the spring, but this time it ferments in the bottle.

冬天寒冷的天气使得发酵过程暂时时停下来,但到了春天又开始发酵,不过这次发酵是在瓶子里进行了。

Amino nitrogen content as protease enzyme capacity indicators was determinated in the fermentation process.

在酱油发酵中,研究了菌株HUV-2的发酵规律并对发酵条件进行探索。

We had made full use of the flavoring components in double bottom distiller′s grains and the strong fermentation properties of Angel brand TH-AADY through application of TH-AADY coupled with the techniques of double bottom fermentation, fermentation of reflux liquor and cross-steaming for thin layers etc.

在较长发酵期的优质浓香型粮食酒生产中,采用TH—AADY与双轮底发酵、回酒发酵、薄层串蒸等工艺相结合,充分利用双轮底糟中香味成分及安琪牌TH—AADY的强发酵性能,有效地提高了粮食酒的产质量,取得了较明显的生产进步和经济效

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