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The steps for preparing insecticide include: first shaking flask for fermentation, adding amidon, soya bean cake powder, cotton cake powder, protease leather, yeast powder, maize milk, magnesium sulfate heptahydrate, monobasic potassium phosphate, dibasic potassium phosphate, calcium carbonate alpha-amylase and make-up water by ratio in culture medium with 100 unit volumes; secondarily filling 100ml culture mediums in triangular bottle, then seeding BTH-13 after disinfection and culturing them until the brood-cell pulls off under finite temperature; third adopting the selected culture medium and fermenting them in full-automatic fermentation tank, acidifying the fermentation liquor after the fermentation, adding the emulsifier, and directly performing spray-drying after the compression of the fermentation liquor to prepare the wettable powder.

制备杀虫剂的步骤是:首先是摇瓶发酵,在100单位体积的培养基中,按比例加入淀粉、豆饼粉、棉饼子粉、蛋白胨、酵母粉、玉米浆、七水硫酸镁、磷酸二氢钾、磷酸氢二钾、碳酸钙、及α-淀粉酶,补充水;其次是在三角瓶中装100ml培养基,灭菌后接种BTH-13,在一定温度培养至芽孢脱落;第三是采用筛选出来的培养基,在全自动发酵罐中进行发酵,发酵结束后发酵液酸化,加入乳化剂,酵液浓缩后直接喷雾干燥制备可湿性粉剂。

This experiment on the fermentation of multiple water samples for coliform determination of three phases: the beginning of the fermentation test, flat panel to verify the separation and recovery fermentation, fermentation of the final restoration coliform test there exist, then in accordance with the beginning of the fermentation tube tests positive the greatest possible number of investigations the number of coliforms coliform report the number of keys.

本实验中阐述的多管发酵法对水样中大肠菌群的测定包括三个阶段:初发酵试验,平板分离和复发酵验证,经最后复发酵试验验证实有大肠菌群存在后,再根据初发酵试验的阳性管数查大肠菌群最大可能数检索表报告大肠菌群数。

The invention use an alcaligenes faecalis as a fermentation strain, using sucrose or glucose commonly used in food industry as carbon source for microbe cultivation, using conventional chemical fertilizer in the agricultural production as nitrogen source and inorganic salt, the medium of microbial growth is prepared by auxiliary yeast powder, aerobic fermentation is processed in the conditions of 30 DEG C, 1.0vvm, 500r/min ventilatiing and stirring, adding specially supplementary food fluid in the fermentation course achieves the following aims:(1) controlling the penetrating pressure of the broth in the hypotonicity condition;(2) controlling the pH of the broth between 4.0 and 7.0;(3) providing abundant nutritious matter such as carbon source and nitrogen source for microbial growth, obtaining Weilan gum broth after 72 hours, obtainin Weilan gum product by drying in 95-115 DEG C and atmospheric pressure.

本发明以一株粪产碱杆菌为发酵菌种,使用食品工业中常用的蔗糖或葡萄糖作为微生物培养的碳源,以农业生产中常用的化肥作为氮源和无机盐,辅以酵母粉配制微生物生长的培养基,在30℃以1.0vvm、500r/min通风搅拌条件下好氧发酵,发酵过程中流加特殊配制的补料液以达到以下目的:(1)控制发酵液的渗透压处于低渗条件;(2)控制发酵液的pH处于4.0~7.0之间;(3)为微生物生长提供丰富的碳源和氮源等营养物质,经72小时得到威兰胶发酵液,在95℃~115℃下常压干燥得到威兰胶产品。

Raw fermentation is the process of liquescence, saccharification and fermentation conducting at the same time. By cutting the cook process, besides energy saving, the deoxidized sugar in the broth is in the low level during the whole process, which has the advantage of avoiding the fermenting material to be infected by other bacteria and being suitable for high gravity fermentation.

近年来兴起的生料发酵,是液化、糖化和发酵同时进行的过程,生料发酵无蒸煮工序,不仅大大降低了能耗,而且还原糖始终处于低水平,这有力避免了染菌,可以进行高浓度发酵,提高了发酵罐的生产能力。

The optimal conditions for extracting IDF with lactobacillus and facultative thermophile were: inoculants size 6%, the ratios between the two reagents 1:2, fermentation temperature 37℃, fermentation time 15h; fermentation temperature and fermentation time were significant effecters to the IDF content(P.05); inoculums size and the ratio between the two reagents were of little significance.

5用微生物发酵法提取水不溶性膳食纤维的最佳工艺条件是:接种量为6%,两菌比为1:2,发酵温度为37℃,发酵时间为15h。发酵温度和发酵时间对产品膳食纤维含量的影响显著(P.05),接种量和两菌比的影响不显著。

The type, initial feeding time and concentration of precursor for HV unit formation had great effects on PHBV production in shake flasks. Propionate was selected as the better precursor for HV fraction than valerate after comprehensive survey of these two precursors. The suitable PHBV shaking-flask culture conditions were obtained through the analysis of PHBV fermentation process, the investigation of several factors by using RSA method and the fed-batch experiments. Cell dry weight, PHBV content, PHBV concentration, HV fraction and yield coefficient of HV unit to propionate were 27. 7g/L, 80.7%, 22. 4g/L, 16. 1mol% and 0.50g/g, respectively.

在共聚物PHBV的摇瓶发酵过程中,PHBV中HV组分形成的前体物质的种类、初始加入时间以及加入量是影响PHBV发酵过程的重要因素;在综合比较丙酸和戊酸这两种前体的基础上,选择丙酸作为HV组分合成前体较适宜;通过对PHBV发酵过程曲线分析、影响PHBV发酵指标的各因素的响应面分析以及摇瓶发酵补料试验,得到了较适宜的PHBV摇瓶发酵补料条件,在此条件下,细胞干重、PHBV含量、PHBV浓度、HV组分含量和HV组分对丙酸的产率系数可分别达到27.7g/L、80.7%、22.4g/L、16.1mo1%和

On the base of former work of high-temperaturetolerent ADY production, this paper performed following application experiments.

本文在前报获得耐高温酒精活性干酵母的基础上,(1)进行了主发酵温度为高温40℃的酒精发酵试验,发酵动力学曲线显示,耐高温酒精ADY在主发酵温度40℃条件下,仍具优良的发酵性能,节水节电,适于安全度夏。

Then,a large scale experiment in a 50 L fermentor is held on the bases. After 60 hours' fermentation, the yield of gum is 2.7%,the viscosity is 8 Pa.s.

在此试验的基础上,进行了50 L发酵罐的放大发酵试验,经过约60 h的发酵,发酵后最终产胶率达2.7%,发酵液粘度为8 Pa.s。

In the single factor experiment ,the authors tested the inoculation, the temperature, the Bx and the fermentation time towards the wine ferment peculiarity's affect and the optimum fermenting technical condition were determined .

以皇冠梨为原料,分别对接种量、发酵温度、糖度及发酵时间等四个因素对皇冠梨果酒发酵特性的影响作了试验研究。然后采用L9(34)正交组合试验设计,确定出了皇冠梨果酒发酵工艺条件。

Based on the oxytetracycline production data and with the aid of mathematical statistics method, the dependent factors which affect the antibiotic potency, have been defined.

本文以土霉素生产的实例,应用数理统计方法,找出不同的发酵条件对土霉素发酵单位的影响;同时指出各发酵条件之间的依存关系,定量地找出发酵条件应控制的水平。根据回归方程可以对在不同条件下的发酵单位进行预测和控制,从而有利于发酵单位的提高。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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