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The results showed that the optimum fermentation conditions are as follows: fermentation time 4 days, inoculum size of yeast 2% and addition of amylaceumq 8%, the alcoholicity in fermentation broth 5.33%.

在单因素试验的基础上,用正交试验对五味子发酵工艺条件进行筛选,得出最优发酵条件为:发酵时间为4d,酵母菌接种量为0.2%,葡萄糖加入量为8%,发酵原液酒精度达到5.33%。

To investigate the mechanisms of biohydrogen production by anerobic fermentation of solid organic waste, gas chromatography is used to analysis the biogas and volatile fatty acid in anaerobic bio-reactor. Firstly, the ability of hydrogen production by digested sludge from the West Lake, Enteobacter aerogences, digested sludge from sewage farm, the fluid of methane pool and dejecta have been studied. The rule of the ability of hydrogen production by different bacteria under different control condition. Simultaneity, the importance of the synergistic effect on hydrogen production has been proved.

本文首先选用西湖底的厌氧活性污泥、产气肠杆菌、污水处理厂的淤泥、沼气池发酵液以及猪粪等不同菌群对废弃食物——马铃薯进行厌氧发酵产氢特性实验研究,得到马铃薯在各不同菌群及工况下的发酵产氢能力,同时发现产氢菌间的协同作用很重要,在控制好发酵条件的情况下,产氢菌群发酵通常会好于单一产氢菌的发酵。

Primary ampliative fermentation was carried out in 10L fermenter containing 5L fermentation medium; the agitation speed kept at 200rpm and the aeration rate being 5L/min; the pH kept at 5.0 while the temperature being 28℃.

在10L罐发酵中进行了初步放大发酵研究,确定了比较合适的发酵条件为:装液量为5.0L,温度28℃,通气量为5L/min,发酵过程中控制pH5.0,转速为200rpm。

Factors for the content of higher alcohols include composition of malt liquid, yeast species and its inoculative use level, and fermentation conditions (fermentation temperature, fermentation methods, fermentation degree etc.).

影响高级醇含量的因素有酵母菌种及接种量、麦汁成分和发酵工艺(如发酵温度、发酵方法、发酵度等)。

4Mm harvested in preculture, is the best. In this form, Rhizopus oryzae R1021 produced most L-lactic acid (76. 62g/L), the pellet size in production medium in flask and fermentor are 1. 9mm and 2. 6mm, respectively, and its L-lactic acid productivity reached or exceeded that of the immobilized cell with polyurethane foam. The produced L-lactic acid and the yield of L-lactic acid based on the total glucose and the productivity are above 74g/L and 74% and 2. 69g/L h, respectively, in 7 batches repetitive fermentation in fermentor, and the size of pellets remain 2. 6mm~3. 4mm.

摇瓶种子培养获得的菌球直径为1.4mm时,这种大小的菌球产酸最高,为76.62g/L;研究表明以直径1.4mm的菌球为种子,在摇瓶发酵和发酵罐发酵中菌球大小分别为1.9mm和2.6mm;这种菌球的产酸水平最高,可以达到甚至超过聚氨酯泡沫载体固定化细胞发酵乳酸的水平;在反复7批次发酵罐发酵过程中,菌球的大小始终保持在2.6mm~3.4mm之间,L-乳酸产量、得率和生产能力分别保持在74g/L、74%和2.69g/L h以上。

The methanol contents of carambola wine fermented from fresh and frozen carambola juice were 33 and 74 ppm respectively. The alcohol content, soluble solid, specificity gravity, pH and titrable acid are almost the same during the fermentation of carambola juice and puree, the methanol content of the must would increase as the fermentation progressing. The methanol content of the wine fermented from carambola juice and carambola puree were 74 and 256 ppm respectively, so the separation of the pulp from carambola puree can decrease the methanol content efficiently in carambola winemaking.

新鲜及冷冻杨桃汁经发酵后,甲醇含量分别为33及74 ppm,杨桃汁与杨桃泥在发酵期间其酒精度、可溶性固形物、比重、pH、可滴定酸的变化情形相似,而甲醇则随著发酵时间增加而增加,杨桃汁及杨桃泥所酿造之杨桃酒其甲醇量分别为74 及256 ppm,结果显示杨桃发酵前汁渣分离将可有效降低杨桃酿造酒中甲醇含量。

Through the research of stability of the fermentation broths of actinomycete strain HJ1-2 to heat, acid. alkali and light, it had a stable activity to heat, acid and UV, but unstable to alkali.

发酵液的稳定性测定结果表明,发酵液对热、酸和紫外线均较稳定,发酵液在800℃处理后抑菌活性开始下降,发酵液在pH3~9条件下抑菌活性稳定。

The experiments indicate that using the dungs and straws to produce hydrogen are feasible.

实验结果表明,在厌氧条件下,采用批量发酵,控制发酵温度为25℃左右,以农作物秸秆、畜禽粪便作为原料,利用沼气发酵后的厌氧活性污泥作为天然产氢菌种来源,以乳酸调控发酵料液pH值在4.5~5.5之间,可以产生氢气。

The compound fermented wine was produced with Gastodia elata BI, Rosa roxburghii Tratt and honey as main materials.

天麻、刺梨、蜂蜜复合发酵酒以刺梨、天麻、蜂蜜为主要原料,将天麻、刺梨分别榨汁,混合调酸,用蜂蜜调糖,经加热、杀菌、冷却后接种训化培养后的酒母,前发酵7d,后发酵20d,再经澄清、过滤、杀菌即得天麻、刺梨、蜂蜜复合发酵酒。

Sterilization in desinfection chamber and subcultures of collections ;②confection of fermentation media and sterilization technics;③influences to objective produce altering the fermentable conditions ,including the examinations of the parameters about remnant sugar、pH、growth of microorganisms and produce;④separations and distillations of objective produces in fermented broth,the distillation technics of filter press and ion exchange;⑤brief and operation techniques of automatic fermentation tank.

课程内容:本课程主要实验内容:①微生物无菌室灭菌及种子扩大培养技术;②微生物发酵培养基及配制及灭菌技术;③微生物发酵及发酵条件的改变对目的产物代谢的影响,包括菌体生长、pH、残糖和产物等发酵过程中各项参数的检测;④发酵液中目的产物的分离及提纯,板框压滤离子交换提取技术;⑤自动发酵罐的简介及操作技术。

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