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The polysaccharide and glucan contents were analyzed by phenol-sulfuric acid method and aniline blue dye fluorescence assay, respectively.

首先将液态灵芝发酵液和商业灵芝粉以酚硫酸法测多醣产量,再以萤光分析方法进行葡聚醣定量。

The interference of fermentation broth to CTAB measurement was further observed. It is revealed that Mg2+ concentration exhibits a significant impact on CTAB results, but glucose, arabinose and D-glucuronic acid not.

进一步研究了发酵液各成分对改良CTAB方法的干扰,结果表明,葡萄糖、阿拉伯糖、D葡萄糖酸前体等对CTAB浊度法没有干扰,但Mg2+等具有较为明显的干扰。

This paper discussed the optimal conditions for the determination of residual alcohol in the fermented mash for Chinese highly-flavored liquor, using potassium bichromate colorimetry.

研究了重铬酸钾比色法检测浓香型白酒发酵糟醅中残余酒精含量的最佳测定条件。

The contents of γ-amino butyric acid in douchies, collected from various regions of China, were analyzed by high-performance liquid chromatography in this study.

采用高效液相色谱法测定全国不同地区传统发酵生产的豆豉样品中的γ-氨基丁酸含量。

We used ion-exchange method to remove butyric acid from fermentation broth.

采用离子交换法吸附发酵液中的丁酸,从18种树脂中筛选出了1种对丁酸吸交性能较好的树脂A。

The detection limits were 0.05μg/ml for cadaverine,histamine and spermidine, 0.1μg/ml for tyramine, 0.25μg/ml for spermine. The recoveries rate of the method were 96.6%,101.94%,101.90%,98.65%,107.4% for cadaverine, histamine, tyramine, spermidine and spermine, respectively.

通过在发酵香肠中强化接种弯曲乳杆菌6031和肠膜明串珠菌51-5 D单一和混合菌种,使用高效液相色谱法检测了7种生物胺含量的变化,同时进行乳酸菌、球菌、总细菌、肠杆菌的计数。

A method for simultaneous determination of 8 organic acids in fermented Capsicum by reverse phase high performance liquid chromatography was developed.

建立了高效液相色谱法同时分析发酵辣椒中8种有机酸的方法。

METHODS Polysaccharide of G.lucidum were extracted and purified.The content,molar ratio and protein content of polysaccharide were determined by HPLC and Kjeldahl method.

提取和纯化灵芝子实体、菌丝体和发酵液多糖,应用高效液相色谱法和凯氏定氮法对多糖的含量、单糖组成及糖蛋白质含量作了分析、比较。

The results showed that Kaidimycin was chemically neutral, macrocylic polylactones, stable and liable to dissolve into water and pyridine, slight to dissolve into methanol, ethanol and N-butanol, difficult to dissolve into ethyl acetate, acetone, benzene and chloroform. Chemical reaction of functional group of Kaidimycin showed that it had glycoside, amino acid and aldehyde, no reducing sugar, polypeptide and phenol.

凯地菌素属中等极性的、中性抗生素,极性纸层析结果与多烯类抗生素的纸层析结果相似:易溶于水、吡啶,微溶于甲醇、乙醇、正丁醇,不溶于乙酸乙酯、丙酮、苯、三氯化碳;对高温、光照和紫外处理有很好的稳定性;官能团反应试验结果发现,凯地菌素可能含有糖苷键、氨基酸伯氨基和醛基,不含有还原糖、多肽和酚基,高锰酸钾、浓硫酸反应与多烯大环内酯类抗生素的特性相似;并且依据纯品,采用生物法和分光光度法对摇床发酵液进行了效价的测定。

Based on the plant growth rate and inhibition zone methods, the antifungal activity of extracts of every organ of Xanthium sibiricum, the fermentation liquid of endophytic fungi MG4-23, and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively. The extract of every organ of X.

方法]采用生长速率法和抑菌圈法,分别以水稻纹枯病菌等6种植物病原真菌和大肠杆菌等3种病原细菌为供试菌种,对苍耳各器官提取液、植物内生真菌MG4-23的发酵液及菌丝体提取液进行抑菌活性测定。

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