发荧光的

Also, the findings of histopathology and direct immunofluorescence examination in some patients were evaluated.

方法临床收集病例，进行性别、年龄、病期、皮疹分布，皮疹形态，症状、伴发疾病等的分析，同时作皮疹的组织病理及直接免疫荧光检查。

Result shows that there is no significant difference in distribution of any two sections' sympathetic nerve in normal goats annulus fibrosus disci intervertebralis,there is no significant difference between the sympathetic fibers distribution of convex side and concave side in annulus fibrosus disci int ...

方法先建立山羊脊柱侧凸模型，将椎间盘纤维环分为左前、左后、右前、右后四个区，然后通过NF200常规荧光组化和乙醛酸染色来研究正常山羊、侧凸山羊、正常人和青少年特发性脊柱侧凸患者椎间盘不同部位纤维环的交感神经分布特点。

Methods Fluorescence in situ hybridization with a whole chromosome 7 painting probe and a 7q subterminal probe (7q36→qter), prepared by chromosome microdissection technique, was used to analyze a case with a history of spontaneous abortion and 7q terminal deletion detected by conventional G-banding technique.

显微切割制备7号和7q亚端粒(7q36→qter)探针，通过荧光原位杂交技术分析一例具有反复流产史且常规G带发现有7q末端缺失的病例。

Methods Prevention and cure measures to control contagion were put into practice.Malaria and skeptical cases were investigated in population.Secondary cases were prevented.Incidence of Malaria was investigated by random sample method with IFAT and density of vector was also investigated;Epidemic trend was analyzed.

实施以控制传染源为主的防制策略，在各类人群中开展疟疾病例侦查，防止继发病例；采用间接免疫荧光技术随机抽样调查居民患病情况和媒介密度；综合分析疟疾流行趋势。

PartⅡ: Analysis of the paraffin embeded tissue slides [obtained from trail and control group at pre-treatment (d0) and post-operation (d15±1 days) ] and frozen section slides (from 4 typicalcases: 1 of IAC group, the other sBRM group at d5, d7 and d10), by H-E stain, monoclonal antibodies (CD3/CD4/CD8/CD68/CD57) marking, SP-DAP or APAAP-AEC stain, and TUNEL in situ apoptotic cell DNA fragments fluorescin labeling.

二。组织病理学研究：对上述两组病人治疗前后原发灶肿瘤组织蜡块分别作H-E和CD3、CD68表型免疫组化染色；并对其中四例典型病人的疗中间断连续活检新鲜组织作H-E染色，CD3、CD4、CD8和CD57表型免疫组化染色和凋亡细胞DNA断片TUNEL法荧光标记检测。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

Blue-light emitting polymer with binaphthalene and PPV moieties was synthesized, which had fluorescence efficiency of 45% in chloroform.

通过将联二萘和PPV单元共聚得到了线性的聚合物发蓝光材料，在溶液中具的荧光量子产率为45％。

Shape of fluorescence spectrum of diphenylanthracene in multilayer film is almost the same as that in solution, and the position of emission peak does not show apparent shift, which is related to the fact that fluorophore are surrounded by highly branched dendrons. These results provide a new route for design and assembly of luminescent materials.

溶液中的荧光光谱和多层膜的荧光光谱的形状和峰位都非常接近，这和发色团处在树状分子的三维支化结构的中心是直接相关的，这一研究对发光材料的分子设计具有重要的指导意义。

A group of constitutive-expression fusions with different fluorescent strength were obtained.

将重组质粒电转化7653R，获得的转化子发不同强度的绿色荧光。

Therefore, the EL spectra of (Znq_2)_4 is wider than that of Znq_2. 4._2 was synthesized. The analysis of molecular spatial structure and the characterization of material performance of_2 and Liq showed that two Liq molecules and two Naq molecules were connected by Na-O-Na bond bridges to form_2. Compared with Liq,_2 exhibits stronger rigidity in planar molecular structure, larger steric hindrance and intermolecular distance, and much smaller molecular polarity, thus resulting in much longer fluorescence lifetime, much higher fluorescence quantum efficiency, wider energy bandgap and better film formability. When used as light-emitting layer in OLED,_2 shows lower formation probability of excited dimmer and exciplex formation than Liq, thus emits bluer light with higher current efficiency than Liq. When_2 ultrathin film is used as electron injection layer in OLED, it exhibits higher current density, higher luminance, lower turn-on voltage and higher current efficiency than Liq ultrathin film for the existence of sodium ions in_2 ultrathin film. 5. The summarization the relationship between molecular spatial structure and material performance of Alq_3,(Znq_2) and Liq, lead the conclusion that the molecular spatial structure of Mq_n affects its material performance in such aspects as the rigidity of planar molecular structure, intermolecular interaction, molecule stacking mode and intermolecular distance.

制备了_2，通过对_2和Liq的分子空间结构与性能进行分析与讨论，发现_2是通过两个Na-O-Na键桥将两个Liq和两个Naq连接构成的，其分子平面结构的刚性程度强于Liq，空间位阻大于Liq，分子之间的距离大于Liq，分子极性远远小于Liq；_2的荧光寿命长于Liq，荧光量子效率高于Liq，成膜性优于Liq；_2的禁带宽度比Liq大，光致发光光谱中_2的最大发射峰较Liq发生蓝移；当_2在OLED中作为发光层时，激发二聚体与激基复合物的生成几率远远小于Liq，发的光比Liq更蓝，电流效率大于Liq；_2超薄膜中有Na离子的存在，与Liq超薄膜相比，当其在OLED中作为电子注入层时，具有更大的电流密度，更高的发光强度，更低的阈值电压和更高的电流效率 5、对上述Alq_3、Znq_2和Liq的分子空间结构与材料性能之间的关系进行了归纳总结，认为Mq_n的分子空间结构主要在分子平面结构的刚性程度，相邻分子之间的相互作用，分子堆叠的方式和分子之间的距离这四个方面影响其性能。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。