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Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P.01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48 and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%. The overall sensitivity was significantly higher (P.05) than that of modified indirect MAIPA and that of using single antibody.

结果 特发性血小板减少性紫痕组4种羊抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P.01);若将ITP组患者4种羊抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性,则流式微球技术检测血小板特异性自身抗体的敏感性为73.2%,特异性为94.3%;4种羊抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P.05),且大于各单个抗体检测敏感性。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P .01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48, and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%.

结果 特发性血小板减少性紫癜组4种单抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P<0.01);若将ITP组患者4种单抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性,则流式微球技术检测血小板特异性自身抗体的敏感性为73.17%,特异性为94.3%;4种单抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P<0.05,且大于各单个抗体检测敏感性。

Hybridization of DNA fluorescent probe modified by coumarin deriving from naphthalene has been researched.

对萘系衍生的香豆素类荧光发色团进行了DNA荧光探针杂交实验的研究。

Effect of water stress on the chlorophyll a fluorescence parameters of flag leaf of the wheat was studied by using OS 30 chlorophyll fluorometer.

作者中文名:张秋英;李发东;刘孟雨;董宝娣摘要:利用OS-30型叶绿素荧光分析仪研究水分胁迫对小麦旗叶叶绿素a荧光动力学参数的影响。

Nevertheless, its fluorescence is enoughintense to be investigated the switch properties in aqueous solution. 5. The results of fluorimetric titrations and spectrophotometric titrations indicate that,for 〓 and 〓, fluorescence of fluorenyl was quenched by transfer of electron from metalcenter to photoexcited state of fluorenyl during coordination of Cu or Ni.

在酸性条件下,未配位的〓和〓都有荧光,在中性及弱碱性条件下与金属铜、镍离子配位后,发生光诱导电子转移,电子由金属离子中心转移至激发态的芴基而使其荧光猝灭。

Objective To investigate the characteristics of fundus photography and fundus fluorescein angiography of IRVAN (idiopathic retinal vasculitis, aneurysms, and neuroretinitis) syndrome and Eales disease.

目的 观察特发性视网膜血管炎、动脉瘤、视神经视网膜综合征和Eales病荧光素眼底荧光血管造影特征的异同。

Absorption spectra indicate that there is little or no interaction between chromphores in the ground state. However, the fluorescence emission of oxadiazole and triphenylamine moieties are almost quenched, which shows that in the excited ground state there exists very effective intramolecular singlet-singlet energy transfer .

研究发现,这些多发色团化合物在基态发色团之间没有作用或作用很小,而其中噁二唑和三苯基胺单元的荧光被严重的猝灭,这说明在激发态下,萘酰亚胺发色团与噁二唑、三苯胺功能团之间发生了分子内单线态—单线态能量转移。

Absorption spectra indicate that there is little or no interaction between chromphores in the ground state. However, the fluorescence emission of oxadiazole and triphenylamine moieties are almost quenched, which shows that in the excited ground state there exists very effective intermolecular singlet-singlet energy transfer.

研究发现,这些多发色团化合物在基态发色团之间没有作用或作用很小,而其中恶二唑和三苯基胺单元的荧光被严重的猝灭,这说明在激发态下,萘酰亚胺发色团与恶二唑、三苯胺功能团之间发生了分子内单线态-单线态能量转移。

Respectivey, The methylmercury levels of human hair for mercury mine workers, children and pregnam women living near the mine area have been compared with that of those living at nonpol luted .

本文采用气相色谱法测定人发中甲基汞,对人发的酸度、浸取时间、人发与酸液的比例,以及无机汞存在的影响作了测定和选择,方法的平均回收率96.6%,精确度2.8%,方法可靠性用氢化物原子荧光法核对,结果一致。

The results reveal that two energy transfer paths (i. e.β-PEB→α/β-PCB and β-PEB→α/βPCB′) with time constants of 50ps and 200ps, respectively, exist in R-PC monomers, and that α/β-PCB′chromophores are the terminal emitting acceptors with fluorescence lifetimes of 1150 ps. The experimental results of time-resolved fluorescence spectroscopy in case of R-PC trimers exhibit that the corresponding energy transfer processes are faster in R-PC trimers than in R-PC monomers, with time constants being 45ps, 165 ps and 920ps.

结果显示:在R-PC单体中存在2条能量传递途径,即β-PEB→α/β-PCB和β-PEB→α/β-PCB′能量传递过程,其时间常数分别为50ps和200ps;作为终端发色团的α/β-PCB′的荧光发射时间常数为1150ps.R-PC三聚体的时间分辨荧光光谱实验结果显示对应的能量传递过程速率加快,其时间常数分别为45ps,165ps和920ps。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。