发荧光

Also, the findings of histopathology and direct immunofluorescence examination in some patients were evaluated.

方法临床收集病例，进行性别、年龄、病期、皮疹分布，皮疹形态，症状、伴发疾病等的分析，同时作皮疹的组织病理及直接免疫荧光检查。

Result shows that there is no significant difference in distribution of any two sections' sympathetic nerve in normal goats annulus fibrosus disci intervertebralis,there is no significant difference between the sympathetic fibers distribution of convex side and concave side in annulus fibrosus disci int ...

方法先建立山羊脊柱侧凸模型，将椎间盘纤维环分为左前、左后、右前、右后四个区，然后通过NF200常规荧光组化和乙醛酸染色来研究正常山羊、侧凸山羊、正常人和青少年特发性脊柱侧凸患者椎间盘不同部位纤维环的交感神经分布特点。

Moreover, these nanoparticles were modified with amine groups and phosphonate groups in one step, and further labeled with galactose receptor.

五、制备了一种发蓝色荧光的半导体纳米晶体，促进了短波长发光纳米材料的相关研究进展。

The emission of light that does not derive energy from the temperature of the emitting body, as in phosphorescence, fluorescence, and bioluminescence.

发冷光：不是由于发光体的热量而来的光的发射，比如在磷光，荧光和生物性发光中。

Methods Fluorescence in situ hybridization with a whole chromosome 7 painting probe and a 7q subterminal probe (7q36→qter), prepared by chromosome microdissection technique, was used to analyze a case with a history of spontaneous abortion and 7q terminal deletion detected by conventional G-banding technique.

显微切割制备7号和7q亚端粒(7q36→qter)探针，通过荧光原位杂交技术分析一例具有反复流产史且常规G带发现有7q末端缺失的病例。

Methods Prevention and cure measures to control contagion were put into practice.Malaria and skeptical cases were investigated in population.Secondary cases were prevented.Incidence of Malaria was investigated by random sample method with IFAT and density of vector was also investigated;Epidemic trend was analyzed.

实施以控制传染源为主的防制策略，在各类人群中开展疟疾病例侦查，防止继发病例；采用间接免疫荧光技术随机抽样调查居民患病情况和媒介密度；综合分析疟疾流行趋势。

PartⅡ: Analysis of the paraffin embeded tissue slides [obtained from trail and control group at pre-treatment (d0) and post-operation (d15±1 days) ] and frozen section slides (from 4 typicalcases: 1 of IAC group, the other sBRM group at d5, d7 and d10), by H-E stain, monoclonal antibodies (CD3/CD4/CD8/CD68/CD57) marking, SP-DAP or APAAP-AEC stain, and TUNEL in situ apoptotic cell DNA fragments fluorescin labeling.

二。组织病理学研究：对上述两组病人治疗前后原发灶肿瘤组织蜡块分别作H-E和CD3、CD68表型免疫组化染色；并对其中四例典型病人的疗中间断连续活检新鲜组织作H-E染色，CD3、CD4、CD8和CD57表型免疫组化染色和凋亡细胞DNA断片TUNEL法荧光标记检测。

Absorption spectra indicate that there is little or no interaction between chromphores in the ground state. However, the fluorescence emission of oxadiazole and triphenylamine moieties are almost quenched, which shows that in the excited ground state there exists very effective intermolecular singlet-singlet energy transfer.

研究发现，这些多发色团化合物在基态发色团之间没有作用或作用很小，而其中恶二唑和三苯基胺单元的荧光被严重的猝灭，这说明在激发态下，萘酰亚胺发色团与恶二唑、三苯胺功能团之间发生了分子内单线态－单线态能量转移。

Here we introduced oxadiazole moiety to theside-chain of Ph-PPV to enhance the electron transport properties. The luminescenceefficiency and luminescence brightness (1.33cd/A, 9400 cd/m~2) of the Ph-PPV withside oxadiazole moiety are much higher than non-oxadiazole Ph-PPV (0.70 cd/A,2300 cd/m~2), and these polymers emitted green light which unaffected by theintroduction of oxadiazole moiety. Anthracene has a rigidity and planar structure which shows high photoluminescenceand thermo-stability.

结果表明，以十烷氧基作为间隔基使噁二唑单元键接在苯基取代PPV的侧链，所得材料制备的电致发光器件的效率和亮度(1.33cd／A，9400 cd／m~2)明显高于不含噁二唑单元的苯基取代PPV(0.70 cd／A，2300 cd／m~2)，噁二唑单元的引入并不影响材料的发光光色，膜状态下的荧光发射光谱和电致发光光谱均呈绿光发射。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。