发荧光

Hybridization of DNA fluorescent probe modified by coumarin deriving from naphthalene has been researched.

对萘系衍生的香豆素类荧光发色团进行了DNA荧光探针杂交实验的研究。

RESULTS AND CONCLUSION: The CFSE-labeled spleen lymphocytes were highly expressed green fluoresce with a positive labeling rate of 95%.

结果与结论：CFSE标记的小鼠脾细胞活力良好，荧光显微镜下见全部细胞标记后均发绿色荧光，形态正常，CFSE标记阳性率为95%。

Effect of water stress on the chlorophyll a fluorescence parameters of flag leaf of the wheat was studied by using OS 30 chlorophyll fluorometer.

作者中文名：张秋英；李发东；刘孟雨；董宝娣摘要：利用OS-30型叶绿素荧光分析仪研究水分胁迫对小麦旗叶叶绿素a荧光动力学参数的影响。

Nevertheless, its fluorescence is enoughintense to be investigated the switch properties in aqueous solution. 5. The results of fluorimetric titrations and spectrophotometric titrations indicate that,for 〓 and 〓, fluorescence of fluorenyl was quenched by transfer of electron from metalcenter to photoexcited state of fluorenyl during coordination of Cu or Ni.

在酸性条件下，未配位的〓和〓都有荧光，在中性及弱碱性条件下与金属铜、镍离子配位后，发生光诱导电子转移，电子由金属离子中心转移至激发态的芴基而使其荧光猝灭。

Objective To investigate the characteristics of fundus photography and fundus fluorescein angiography of IRVAN (idiopathic retinal vasculitis, aneurysms, and neuroretinitis) syndrome and Eales disease.

目的 观察特发性视网膜血管炎、动脉瘤、视神经视网膜综合征和Eales病荧光素眼底荧光血管造影特征的异同。

Absorption spectra indicate that there is little or no interaction between chromphores in the ground state. However, the fluorescence emission of oxadiazole and triphenylamine moieties are almost quenched, which shows that in the excited ground state there exists very effective intramolecular singlet-singlet energy transfer .

研究发现，这些多发色团化合物在基态发色团之间没有作用或作用很小，而其中噁二唑和三苯基胺单元的荧光被严重的猝灭，这说明在激发态下，萘酰亚胺发色团与噁二唑、三苯胺功能团之间发生了分子内单线态—单线态能量转移。

Shape of fluorescence spectrum of diphenylanthracene in multilayer film is almost the same as that in solution, and the position of emission peak does not show apparent shift, which is related to the fact that fluorophore are surrounded by highly branched dendrons. These results provide a new route for design and assembly of luminescent materials.

溶液中的荧光光谱和多层膜的荧光光谱的形状和峰位都非常接近，这和发色团处在树状分子的三维支化结构的中心是直接相关的，这一研究对发光材料的分子设计具有重要的指导意义。

The results reveal that two energy transfer paths (i. e.β-PEB→α/β-PCB and β-PEB→α/βPCB′) with time constants of 50ps and 200ps, respectively, exist in R-PC monomers, and that α/β-PCB′chromophores are the terminal emitting acceptors with fluorescence lifetimes of 1150 ps. The experimental results of time-resolved fluorescence spectroscopy in case of R-PC trimers exhibit that the corresponding energy transfer processes are faster in R-PC trimers than in R-PC monomers, with time constants being 45ps, 165 ps and 920ps.

结果显示：在R-PC单体中存在2条能量传递途径，即β-PEB→α/β-PCB和β-PEB→α/β-PCB′能量传递过程，其时间常数分别为50ps和200ps；作为终端发色团的α/β-PCB′的荧光发射时间常数为1150ps.R-PC三聚体的时间分辨荧光光谱实验结果显示对应的能量传递过程速率加快，其时间常数分别为45ps，165ps和920ps。

Therefore, the EL spectra of (Znq_2)_4 is wider than that of Znq_2. 4._2 was synthesized. The analysis of molecular spatial structure and the characterization of material performance of_2 and Liq showed that two Liq molecules and two Naq molecules were connected by Na-O-Na bond bridges to form_2. Compared with Liq,_2 exhibits stronger rigidity in planar molecular structure, larger steric hindrance and intermolecular distance, and much smaller molecular polarity, thus resulting in much longer fluorescence lifetime, much higher fluorescence quantum efficiency, wider energy bandgap and better film formability. When used as light-emitting layer in OLED,_2 shows lower formation probability of excited dimmer and exciplex formation than Liq, thus emits bluer light with higher current efficiency than Liq. When_2 ultrathin film is used as electron injection layer in OLED, it exhibits higher current density, higher luminance, lower turn-on voltage and higher current efficiency than Liq ultrathin film for the existence of sodium ions in_2 ultrathin film. 5. The summarization the relationship between molecular spatial structure and material performance of Alq_3,(Znq_2) and Liq, lead the conclusion that the molecular spatial structure of Mq_n affects its material performance in such aspects as the rigidity of planar molecular structure, intermolecular interaction, molecule stacking mode and intermolecular distance.

制备了_2，通过对_2和Liq的分子空间结构与性能进行分析与讨论，发现_2是通过两个Na-O-Na键桥将两个Liq和两个Naq连接构成的，其分子平面结构的刚性程度强于Liq，空间位阻大于Liq，分子之间的距离大于Liq，分子极性远远小于Liq；_2的荧光寿命长于Liq，荧光量子效率高于Liq，成膜性优于Liq；_2的禁带宽度比Liq大，光致发光光谱中_2的最大发射峰较Liq发生蓝移；当_2在OLED中作为发光层时，激发二聚体与激基复合物的生成几率远远小于Liq，发的光比Liq更蓝，电流效率大于Liq；_2超薄膜中有Na离子的存在，与Liq超薄膜相比，当其在OLED中作为电子注入层时，具有更大的电流密度，更高的发光强度，更低的阈值电压和更高的电流效率 5、对上述Alq_3、Znq_2和Liq的分子空间结构与材料性能之间的关系进行了归纳总结，认为Mq_n的分子空间结构主要在分子平面结构的刚性程度，相邻分子之间的相互作用，分子堆叠的方式和分子之间的距离这四个方面影响其性能。

Green fluorescent protein gene was conjugated to the 3 end of the PAP gene in order to screen easily of the transgenic cotton plants. The combined gene was cloned into plant expression vector pBI121 and then transformed. About 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays. This result reflected that the GFP gene was expressed in the transgenic cotton; dot-blot result also demonstrated that PAP gene was exactly transformed into the cotton. The capacity of the Verticillium dahliae-resistance is being analyzed.

为了便于转基因棉花后代的筛选，在 PAP基因的 3'端融入了绿色荧光蛋白GFP）基因，然后将融合基因克隆在植物表达载体PBI 121上，再进行遗传转化，得转基因棉花种子5000余粒，将种子播种长到于叶展开时，先在黑暗中用紫外灯照射，查找表现绿色荧光的幼苗，然后再用地高辛标记的PAP基因特异性探针对这些棉花进行点杂交，最后发现有8株棉花表现阳性反应，说明PAP基因的确己经转到了棉花的基因组中，其棉花黄萎病的抗性鉴定正在进行之中。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。