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The morphogenesis of floral organs in both srg mutant and wild-type rice Dular (Oryza sativa L.) was observed using the scanning electron microscope. The results showed that the floral primordia were differentiated under irregular pattern in srg mutant. After regular formation of lemma and palea primordia, the floral primordium of srg formed lodicule primordia inner to both lemma and palea, followed by the formation of stamens and carpel. Alternatively, floral primordia in srg were split to form two or more floral primordia. The number and position of stamens and carpels primordia were malformed.

利用扫描电子显微镜观察野生型水稻和裂颖水稻的颖花原基分化情况,结果发现,突变体水稻的幼穗在形成内、外稃原基后,有些颖花在形成浆片后并不是直接发育形成雄蕊原基,而是在外稃或内稃原基内侧又分化出浆片原基;有些颖花原基从中间缢裂,缢裂的颖花原基又分别形成类稃片的结构,颖花发育后期,6枚雄蕊发育畸形,并且观察到不同数目的雄蕊。

The floral primordium is initially circular, along with the edge of which slight concaves occur. The outmost whorl of tepal primordia arises as a result of apical meristem division and the floral primordium then is in the shape of triangle. Two further whorls of tepal primordia arise in spiral acropetal sequence and are alternately arranged. When the innermost whorl of tepal primordia is at the late developmental stage, the foremost stamen primordia arise almost simultaneously in spiral acropetal succession and all of the stamen primordia are ultimately arranged in a triangle-cone form. Late in stamen primordium development, carpel primordia arise as same as the young stamen primordia and grow laterally. Grooves are present on the adaxial side of each carpel and develop into ventral sutures gradually closing up.

观光木的花原基最初为近圆形,随着顶端分生组织的活动,花原基边缘处出现浅凹,形成第一轮花被片原基,此时,花原基呈三角形排列,后两轮花被片原基依次发生,与前一轮互生;在内轮花被片发生的后期,最初几枚雄蕊原基几乎同时出现,呈螺旋状向顶发生,最后排列成三角圆锥状;雄蕊原基发育后期,心皮原基开始发育,形状与发育初期的雄蕊原基相似,随后心皮原基进行侧向生长,在近轴面出现浅凹,进而发育为凹槽,形成腹缝线,最后腹缝线完全愈合。

RESULTS: The growth speed, weight, developmental uniformity, bionergy and fecuncity of the Bombyx silkworm descent dramatically as they eat the feed that the concentration of 2,4-DCP over 0.8 mmol L~-1 or NP exceed 0.500 mmol L~-1. But the effect on reproductive capacity vary with gender difference, it's more obvious on the late-stage of development and periods of metamorphosis such as ecolysis, pupation, edosion. There may be cumulative effect inside the Bombyx siliworm.

结果 家蚕幼虫食下含0.8 mmol·L~(-1)以上浓度的2,4-DCP或0.500 mmol·L~(-1)以上浓度的NP饲料,生长速度、生长量、发育整齐度、生命力和生殖能力都明显下降;生殖能力所受影响应性别而不同;家蚕发育后期和蜕皮、化蛹、羽化等变态时期影响更加明显,在蚕体内可能有累积作用。

The expression profiles analysis of 25 genes belonging to 8 types in different development periods of Y35 somatic embry showed, genes involved in transcription, signal transduction, cell fate, substance transport, cell and development regulation were active at early and middle stages of somatic embryogenesis, while at the stage of cotyledonary embryo maturation, the high level-expressed genes were mainly those related to storage protein, maturation and defense.

通过半定量和Real-Time PCR技术,对WRKY和MAPKK等8类25个差异表达基因在Y35体细胞胚不同发育时期的表达分析显示,转录、信号转导、细胞生长分裂、细胞命运、物质转运和多数发育调节相关基因在体细胞胚胎发生的早期或中期比较活跃,而进入子叶胚成熟后期则主要是储藏蛋白、成熟和防御相关基因大量表达。

This suggests persistent expression of SiDIV and ceasing of SiCYC1A and SiCYC1B in late peloric flower stage may generate the actinomorphic flower.

综此推测,变异型的花发育成辐射对称可能是由於开花晚期时SiCYC表现量下降及SiDIV持续表现,造成花发育后期两侧对称性的无法继续维持,而成了辐射对称花形式。

The results shows that the tapetal expanding abnormally occurs in dyadphase stage, microspore abortion occurs in the early of monokaryon stage, disaggregation in the end of monokaryon stage, only some spall of microspore is observed in monokaryon stage, the four cell walls also exist, the tapetal cells became completely vacuolated, almost filled the whole locule. vascular bundle of male sterile line anther vanish at mature pollen stage.

结果发现花药绒粘层在二分体时期发生异常,小孢子在单核前期开始败育,至单核后期完全解体,花药发育后期不育系药腔中只观察到少量的小孢子残体,花药壁的四层细胞依然存在,绒粘层细胞已经彻底液泡化,占据了药腔的整个空间,花粉成熟期不育系花药维管束消失。

Using the scanning electron microscope, the starch granules were observed which gathering and stuffing in scale cell during bulb development.

用扫描电镜观察到鳞片细胞中明显的淀粉颗粒,并随鳞茎的发育充满整个细胞腔。14C示踪技术分析表明,含苞期的14C-同化物分配以地上部为主,集中在花苞部位;花后期的14C-同化物分配以地下鳞茎为主,其中下位叶标记处理的鳞茎分配量占植株总14C的比例达85.8%,证实了植株的光合产物在花后期主要供应鳞茎发育膨大所需。

The results indicated that the SS expression in sweet sorghum leaves and stems was lower at early growth stages, increased dramatically at late growth stages (flowering, grain filling, and dough stage), and reached the highest level at flowering stage.

结果表明,甜高粱叶片和茎秆中的SS蛋白质表达量在发育早期较低,发育后期(开花期、灌浆期和腊熟期)明显升高,在开花期最高。

Mysoin, the protein that allows muscle to contract, was known to be essential for the beginning of development in certain amoebas, but its importance in subsequent stages was unknown.

肌浆球蛋白是一种促使肌肉结合在一起的蛋白,众所周知,这种蛋白是特定变形虫开始发育的必要物质,但是它在后期发育阶段的重要性并不为人所知。

Compared with the ck, the sugar accumulation increased extremely significant by grafting with the wild watermelon 2, but that of the other treatments all decreased and the sugar accumulation of loofah/Zaojia was the lowest. The sucrose invertase activity decreased; the activitives of sucrose phosphate synthase and net for sucrose-metabolism enzymes all increased during the development of own-rooted and grafted watermelons.

与对照相比,野生西瓜2号/早佳组合的果实糖积累极显著增高,而其他嫁接组合的糖积累均降低,丝瓜/早佳组合的果实糖含量最低;自根和嫁接西瓜果实发育过程中蔗糖转化酶活性降低,蔗糖磷酸合酶活性升高,蔗糖合酶在西瓜果实发育后期主要起合成作用,促进果实蔗糖积累,蔗糖代谢相关酶净活性值增大。

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