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Rresults from those studies can be summariazed as follow:(1) A preliminary study no correlationship between the UV-VIS reflection spectrum of tomato fruit surface and the lycopene amount of tomato fruit was undertaken. Based on observation from this study, a damage-free and in-situ detection technique of lycopene amount from tomato was developed with UV-VIS reflection spectroscopy;(2) The geometrical isomers of lycopene were separated successfully on C30-HPLC-PDA-ELSD. The absorption coefficients of Z-isomers were consequently dedermined. The geometrical isomer composition of lycopene could therefore be assessed. Those efforts formed a solid base to identify the natural product from synthetic compound of lycopene;(3) Lycopene resource from the fruit of Autumn oliver was proved to be available for industrial application. An extraction methos of lycopene from the fruit of Autumn oliver by supercritical carbon dioxide was developed at laboratory scale;(4) A method to extract lycopene by supercritical liquid with improved efficiency was developed; Data from this investigation suggested that a proper raw material pre-extraction process was important for a better extraction efficiency;(5) Variation in the geometrical isomer composition of lycopene during its metabolish in rat was assessed. A large amount of Z-isomers were observed. This variation took place in serum;(6) The absorption rate and accumulation in serum of natural lycopene in rat were assessed;(7) Ability to quench singlet oxygen by different geometrical isomers of lycopene was compared;(8) It was detected that whether natural lycopene had functions to regule blood-lipids and LDL antioxidation;(9) A C31 degradation piece of lycopene was found in the prostate gland of rat.

研究结果包括:[1]应用UV-VIS反射光谱法建立了番茄果实中番茄红素含量的无损伤、现场检测技术;[2]应用C30-HPLC-PDA-ELSD技术实现了对番茄红素几何异构体的分离,测定了不同顺式异构体的吸光系数,建立了天然番茄红素几何异构体组成的检测方法,为区分和鉴定人工合成品和天然产物奠定了基础;[3]证实了秋橄榄果实中的番茄红素资源具有工业开发价值,并建立了超临界CO_2萃取的实验室方法;[4]探索了提高番茄红素超临界流体萃取效率的方法,证明了物料前处理对提高萃取效率起了极其重要的作用;[5]观察了番茄红素在大鼠体内几何异构体组成的变化情况,发现了大量顺式异构体的存在,并证实了几何异构体组成在血清中发生了显著变化;[6]测定了天然番茄红素在大鼠体内的吸收率和在大鼠血清中的动态积累规律;[7]比较了不同番茄红素几何异构体淬灭单线态氧的能力;[8]检测了天然番茄红素是否具有调节血脂作用和抗低密度脂蛋白氧化的功能;[9]首次发现大鼠前列腺内存在番茄红素C31降解片段,并证实其仍具有淬灭单线态氧的能力。

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