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These new findings are important for improving induction and conversion frequencies in conifer somatic embryogenesis.

因此,这些新的研究成果对于改善松柏类植物体细胞胚胎发生中的胚的诱导率和转换率低的现象具有重要的意义。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明:(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料;(2)在所试验到的MS、MS1/2(将MS的所有成分均减半)、WPM、DKW、B〓、SH等六种基本培养基中,MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基;(3)水曲柳的体细胞胚胎发生以直接发生为主,体细胞胚主要来自于从合子胚分离的完整子叶;(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响,诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2,5,10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0,2mg/L+TDZ 0.05mg/L;(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力,少数愈伤组织可以分化出体细胞胚;(6)愈伤组织的诱导也受培养基和激素配比的显著影响,最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2,5mg/L+TDZ0.05mg/L;(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力,此时种子尚未成熟,胚乳已呈固态,种胚的各个部分已分化完全,种胚体积占胚腔的大约2/3;(8)在各自综合的最适条件下,完整子叶的体细胞胚诱导率可达33.68%,下胚轴的愈伤组织诱导率可达90.7%。

Objective To discuss the application of PDCA circular administration in preventing the occurrence of phlebitis caused by chemotherapeutic drugs.

目的 探讨PDCA循环管理预防化疗药物所致静脉炎的发生中的运用,降低化疗药物所致静脉炎的发生率,减轻患者的痛苦,提高患者对护理工作的满意度。

Barrett's esophagus, the most important risk factor in the deelopment of adenocarcinoma of the esophagus, is a metaplastic condition in which the normal squamous epithelium of the esophagus is replaced by columnar or glandular epithelium.

Barrett食管,是食管的正常的鳞状上皮被柱状或腺状上皮所取代的一种化生状态,为食管腺癌的发生中的最重要的危险因素。

This study is to further investigate the mechanisms of osteoclastic bone resorption induced by IL-1 with established osteoclasts culture and osteoclasts, bone marrow stromal cells co-culture system.

本文利用建立的破骨细胞培养和破骨细胞、骨髓基质细胞共培养体系,在体外进一步研究IL-1诱导破骨细胞性骨吸收的机制,旨在探讨细胞因子在骨质疏松症骨丢失发生中的作用。

Barnes likens his work with the Mice to deciphering a snapshot of an automobile accident in progress.

巴尼斯把他的工作比做破译正在发生中的车祸的快照。

In acoustic neuromas, patients with LOH were younger at the age of diagnosis, and larger tumor size, had shorter history and higher growth rate. Conclusions CHR22 LOH was a frequent event in the tumorigenesis of sporadic schwannoma. The presence of CHR22 LOH was related to clinical features.

CHR22 杂合子丢失是神经鞘瘤发生中的常发事件,缺失的区域包括NF2基因,CHR22 杂合子丢失与听神经瘤的临床行为有一定的关系,CHR22 杂合子丢失可能是神经鞘瘤增殖的重要因素。

Based on the references and our studies, we reviewed the bile injury on esophageal mucosal epithelial cells, the diagnosis and treatment of bile reflux into esophagus. The relationships of the biles with Barrett s esophagus and esophageal adenocarcinoma were elucidated especially.

本文复习文献并结合我们的研究成果,就胆汁对食管黏膜的损伤,包括损伤的可能机制、食管内胆汁反流的诊断、治疗等方面作以介绍,重点对胆汁在Barrett食管和食管腺癌发生中的作用及其防治作了阐述

Based on the references and our studies, we reviewed the bile injury on esophageal mucosal epithelial cells, the diagnosis and treatment of bile reflux into esophagus. The relationships of the biles with Barretts esophagus and esophageal adenocarcinoma were elucidated especially.

本文复习文献并结合我们的研究成果,就胆汁对食管黏膜的损伤,包括损伤的可能机制、食管内胆汁反流的诊断、治疗等方面作以介绍,重点对胆汁在Barrett食管和食管腺癌发生中的作用及其防治作了阐述

Pathologic observation also proved these results .It was suggested that TWP and anti-DC McAb had immunosuppressive effect on AIT.

本文在建立小鼠AIT的基础上,观察雷公藤多甙与DC单抗在抑制小鼠AIT发生中的作用,探讨二者对AIT治疗的可能性。

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Plunder melds and run with this jewel!

掠夺melds和运行与此宝石!

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻,也许正是在通往天国的路上,我体验着这白色的晕旋。

When you click Save, you save the file to the host′s hard disk or server, not to your own machine.

单击"保存"会将文件保存到主持人的硬盘或服务器上,而不是您自己的计算机上。