发生

I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Said you're going with your boys today and "Don't worry cause I'll be home late" But I know you're creepin' out there, cheatin', You've been freakin' babe So let me tell you how it's going to be And you can run game but not on me boy So who the hell are you trying to kid You know this is what I did I called your pager and your two-way boy but no response Start looking and I found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Baby boy I know you'll never find No stuff as good as mine She can't work you like I did You can look but you know I got that good shit I know that you're hating it But you better stay with the one you're with You made your bed and now you have to sleep in it baby I called your pager and your two-way boy but no response Start looking and found you in some other women's arms Don't try to front boy cause I saw you there with my own eyes Just get your stuff boy cause I'm tired of hearing all your lies Bounce baby out the door I ain't gonna take this no more Bounce baby out the door Get up and move Don't make me act a fool Just bounce baby out the door I ain't gonna take this no more I ain't gonna take this no more Bounce baby out the door Bounce baby out the door Get up and move Get up and move Don't make me act a fool Don't make me act a fool Just bounce baby out the door Wait a minute Sarah, give me a chance to explain I was just talking to shorty, I don't even know her name I ain't looking for no one to come and take your place Ain't no one out there baby that can fill your space No need to find a nickel got a dime at home Plus I love every night the freaky way we bone I ain't trying to get gone, ain't trying to roll I'd rather stay and be a part of your green eyed soul Just bounce baby out the door Bounce baby out the door Just bounce I ain't gonna take this no more Just bounce Bounce baby out the door Just bounce Get up and move Just bounce Don't make me act a fool Just bounce

我ain't去向前发生作用这个不更多弹起当作婴儿般对待出去门到达向上和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待出去门弹起当作婴儿般对待出去门我ain't去向前发生作用这个不更多弹起当作婴儿般对待出去门到达向上和移动做不制造我行动a开玩笑假定你是去用你的男孩今天和"做不担心原因我希望在家迟"但是我知道你是creepin'出去那里cheatin'你已经是freakin 婴儿那样让我说出你，它怎样正将是和你能变得勇敢了但是不是在我上那样地狱对是你试一试的男孩哄骗你的知道这个是我所做的我呼吁你的寻呼和你的双向的男孩但是没有看和我反应出发发现你在情妇手臂不努力尝试的一些中我有我的自己眼睛的仅仅把厌烦你的我被的材料男孩原因带到那里听你所有的谎言处理前面的男孩原因你我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门仅仅弹起弹起当作婴儿般对待从里面门我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑小男孩我知道你希望从不裁决不材料实际已经开采矿她不能工作你相似我是做你罐看但是你知道我得到那个好排粪我知道那个你是讨厌它但是你更好些留下用一你是用你制做你的床和现在你富人向睡觉在中它当作婴儿般对待我打电话你的寻呼和你的双向地男孩但是没有反应开始看和发现你在中一些情妇's手臂做不请尝试向前男孩原因我拉来拉去你那里用我自己的眼睛仅仅到达你的材料男孩原因我被使厌烦的听见你所有的谎言弹起当作婴儿般对待从里面门我 ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门到达沿着和移动做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门我ain't去向发生作用这个没有更多我ain't去向发生作用这个没有更多弹起当作婴儿般对待从里面门弹起当作婴儿般对待从里面门到达沿着和移动到达沿着和移动做不制造我行动a开玩笑做不制造我行动a开玩笑仅仅弹起当作婴儿般对待从里面门仅仅弹起等候a分钟萨拉让步我a机会向说明我是仅仅说话向矮子我做不平稳知道她名字我ain't寻找没有任何人向来和发生作用你的地方Ain't没有任何人从里面那里当作婴儿般对待那个罐充满你的是一部分你的绿色是从从门公正弹起里面门公正弹起弹起小里面盯着精神公正弹起小我ain't去把这没有更公正弹起弹起小带到从门里面仅仅弹起组织和移动公正弹起不使我装出一傻瓜公正弹起的样子

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明：(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料；(2)在所试验到的MS、MS1/2（将MS的所有成分均减半）、WPM、DKW、B〓、SH等六种基本培养基中，MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基；(3)水曲柳的体细胞胚胎发生以直接发生为主，体细胞胚主要来自于从合子胚分离的完整子叶；(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响，诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2，5，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0，2mg/L+TDZ 0.05mg/L；(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力，少数愈伤组织可以分化出体细胞胚；(6)愈伤组织的诱导也受培养基和激素配比的显著影响，最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2，5mg/L+TDZ0.05mg/L；(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力，此时种子尚未成熟，胚乳已呈固态，种胚的各个部分已分化完全，种胚体积占胚腔的大约2/3；(8)在各自综合的最适条件下，完整子叶的体细胞胚诱导率可达33.68%，下胚轴的愈伤组织诱导率可达90.7%。

Neuroectoderm; neural crest; neural plate; neural tube; neuraxis; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；神经管；轴索；成神经细胞；神经干细胞；neuroprogenitor细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管缺陷；无脑；脊柱裂；成神经细胞瘤细胞；细胞迁移；神经发生；发展；发育阶段

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；底板；神经管；轴索；成神经细胞；神经干细胞；神经母细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管缺陷；无脑；脊柱裂；细胞迁移；神经发生；发展；发育阶段

Neuroectoderm; neural crest; floor plate; neural tube; neuraxis; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经脊；底板；神经管；轴索；成神经细胞；神经干细胞；神经母细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管缺陷；无脑；脊柱裂；成神经细胞瘤细胞；细胞迁移；神经发生；发展；发育阶段

Neuroectoderm; neural crest; neural plate; floor plate; neuraxis; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层神经脊；神经板；底板；轴索；成神经细胞；神经干细胞；神经母细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；成神经细胞瘤细胞；细胞迁移神经发生；发展；发育阶段

Neural crest; neural plate; floor plate; neural tube; neuraxis; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经脊；神经板；底板神经管；轴索；成神经细胞；神经干细胞；神经母细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管缺陷；无脑；脊柱裂；成神经细胞瘤细胞；细胞迁移；神经发生；发展；发育阶段

Neuroectoderm; neural plate; floor plate; neural tube; neuraxis; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经板；底板；神经管；轴索成神经细胞神经干细胞；神经母细胞；干细胞分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管缺陷；无脑；脊柱裂；成神经细胞瘤细胞；细胞迁移；神经发生；发展；发育阶段

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；底板；神经管；轴索；神经胚形成；成神经细胞；神经干细胞；神经母细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管缺陷；无脑；脊柱裂；成神经细胞瘤细胞；神经发生；发展；发育阶段

In case of 如果发生 In case of fire, open this safety door.

一旦发生火灾时，打开这扇安全门

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。