发现物

In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

The fluorescence anisotropy using steady-state fluorescence polarization method was measured for the solution of β-CD and cinchonine mixed and sonicated for 1h. The anisotropy values for the solution increased with the increase of the concentrations of β-CD, which implied the existence of some complexes of large size or nanotube structure. Many type of large size complexes formed between β-CD and Cin under high concentrations in gas phase were characterized by ESI-MS in moderate experiment conditions.

将辛可宁与环糊精的混合溶液超声1个小时后，用稳态荧光偏振方法测定溶液的各向异性值，发现随着环糊精浓度的增大，溶液的荧光各向异性不断增强，这意味着溶液中形成了大尺寸即纳米级结构的环糊精与辛可宁复合物；用温和的质谱测定条件表征发现高浓度的β-CD与辛可宁在气相中可形成各种类型的大尺寸复合物。

The products amplified by primer 2 had polymorphism. The sequencing results indicated that there were two nucleotide mutations( C→A and C→T )at exon 1183bp and 336bp of hircine GDF9 gene, but the two mutations did not cause amino acid change. The products amplified by primer 3 had polymorphism.

所设计的6对引物扩增产物中共有3对发现了多态，引物1经SSCP分析虽然没有发现多态但经克隆测序却在外显子1编码区前-4bp处检测到A→C的突变。

In the research, we have found the interesting phenomena that NP1+shall+VP (+NP2) type of sentences use more transitive verbs than intransitive verbs and shall + notional verb type of sentences tend to use more verbs of active voice than those of passive voice.

在分析shall引导的实义动词原形句中，我们发现在该句式中及物动词的出现频率明显多于非及物动词的频率；在对shall引导的实义动词句进行分析时，我们发现该类句式中的主动语态句明显多于被动语态句。

AFLP marker technique was used to investigate the genetic relationships between 50 accessions of maize and its wild relatives including teosinte, Tripsacum and Coix With twenty one AFLP primer combinations of PstⅠ/MseⅠ, 641 AFLP amplified bands were obtained, including 586 polymorphic bands with an average of 30 52 per primer combination and the polymorphism percentage of 91 4% Cluster analysis using UPGMA method based on the data of AFLP showed that the 50 materials could be divided into...

用AFLP分子标记技术对 5 0个有代表性的玉米及其野生近缘种大刍草、摩擦禾、薏苡材料进行了遗传关系分析。利用 2 1个PstⅠ MseⅠ引物组合检出可辨认带共 6 4 1条，其中多态性带 5 86条，平均每对引物得到 30 5 2条多态性带，多态性 91 4 %。聚类分析结果表明，可以将 5 0个材料分为 3大类，与已有的玉米族分类一致，但与玉蜀黍属内的种间和种内分类存在差异，说明大刍草各种内存在较大的遗传多样性。本实验发现玉蜀黍属与摩擦禾关系比与薏苡的关系更近，但并没有发现玉米与小颖玉米亚种比与其他大刍草的遗传关系更近。

Under scanning electron microscope,barite in these samples occurs as euhedral crystal,co-existing with authigenic quartz and albite with the same idiomorph.Considering the geological setting and c rystal form,it is believed that barite occurred in the study area is compara ble with that found in the Guaymas Basin,Gulf of California,i.e.,with hydrothermal origin.

通过对东太平洋多金属结核中国开辟区表层沉积物和短柱状沉积物的系统观察和分析，首次根据矿物学和地球化学两方面的证据，发现中国开辟区沉积物中热水活动及其产物的存在。X射线衍射分析表明，沉积物样品中普遍含有重晶石，部分样品中含量高达10%以上。

This paperindicated that it was good for condensation reaction when benzene-ring bearingelectron-attaching groups. It also showed that and the 6-hydroxyl group could bereacted with acyl halide, haloid ether and halo alkene, but not with halo alkane. It wasnot only the first time to find that the reaction product of D-mannitol and aromaticaldehyde is tri-condensation derivatives, but also the first time to obtain thep-phthaldehyde-sorbitol derivatives containing eight hydroxyl groups.

研究发现：苯环上有吸电子基团时对缩合反应有利；多元醇缩醛衍生物6-羟基除与酰氯反应外，也能和卤代酸酯、溴代烯反应，但不与卤代烷反应；首次发现D-甘露醇与醛反应主要得到三缩合物；首次合成了含有8个羟基的对苯二甲醛和山梨醇的缩合物。

To our knowledge, reverse phase of high performance liquid chromatography was firstly developed to analyze the metabolites of 7-tocopherol with ideal results. Meanwhile, Gas chromatography/Mass chromatography were used to analyze the structures of unknown metabolites of 7-tocopherol and a main unknown metabolite was identified as a side chain ω- hydroxylate of 7-tocopherol.The kinetic results of of 7-tocopherol reacted in the S9 of male SD-rat liver showed that the optimal time was 45 min; the optimal concentration of S9 5 mg/ml; the optimal concentration of NADPH 1 mM; and the optimal concentration of 7-tocopherol 8 μg/ml. The result of γ-tocopherol metabolized in the S9 of male SD-rat was the best; male Brazil turtle better; and male cobia worst.

本试验首次确立了γ-生育酚代谢物的RP-HPLC分析方法，结果理想且重现性好；用GC／MS对γ-生育酚的未知代谢物结构进行分析，判断其中一种主要的代谢物为γ-生育酚侧链初级羟基化代谢物；γ-生育酚在雄性SD-大鼠肝S9的代谢动力学表明，本实验条件下代谢反应的最佳时间为45min，S9浓度为5mg／ml，NADPH浓度为1mM，γ-生育酚浓度为8μg／ml；发现γ-生育酚在雄性SD-大鼠肝S9中代谢最高，雄性巴西龟次之，雄性军曹鱼最低。

The results from analysis of conjugated dienes and thiobarbituric acid-reactive substance formation showed that aqueous extracts of black rice and black sesamin and ethanol extracts of black Jew's ear inhibited the oxidation of LDL.

我们发现，黑糯米和黑芝麻水萃取物及黑木耳的酒精萃取物会延迟脂质氧化过程的中间产物 conjugated dienes 的出现，且会抑制终产物 thiobarbituric acid-reactive substance 的产生，反映出黑糯米和黑芝麻水萃取物及黑木耳的酒精萃取物具有抗低密度脂蛋白氧化的能力。

The results show that the as-synthesized chalcone-containing copolymers are a kind of thermosensitive polymer with a lower critic solution temperature and the LCST of the copolymer decreases with the increase of the chalcone content in the copolymer. With the increase of the solvent polarity, both the maximum absorption wavelength and the fluorescence emitting wavelength of the chalcone-containing copolymers redshifts, and the intensity of fluorescence first increases, then decreases, which exhibits solvent polarity sensitive fluorescence properties.

结果表明，侧链查尔酮共聚物是一类具有低临界溶解温度的温敏性聚合物，其LCST温度随着共聚物中查尔酮含量的增加而降低；随着溶剂极性的增加，侧链查尔酮共聚物的紫外-可见最大吸收波长红移，其荧光发光波长红移并且发光强度先增强后降低，具有溶剂极性敏感的荧光特性；同时对比侧链查尔酮共聚物水溶液低温和高温下的荧光，发现低温下几乎无荧光，高温下其荧光得到明显增强，其荧光具有可逆的温度&开/关&特性。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。