发现

Methods In this study,a plasmid vector,rAd5,and rAAV2 were used to transfect rat cochlear marginal cells of stria vascularis by injection into the perilymph through the round window membrane,and the transfection efficiency, target tissue accessibility, cell/ tissue toxicity, time course of expression, and effect on hearing were evaluated in vivo and in vitro.

结果三种载体对原代培养的大鼠耳边缘细胞的转染研究发现，腺病毒的转染效率最高，而腺相关病毒转染对细胞活性影响最小。rAAV2和rAd5对大鼠耳蜗组织的感染实验发现，腺病毒和腺相关病毒携带的增强型绿色荧光蛋白可在多种耳蜗组织中及转染对侧耳蜗组织表达，且并不引起明显的耳蜗组织细胞凋亡。rAAV2携带的EGFP基因在第90天时仍可检测到大量表达。rAd5携带的EGFP基因在第30天时表达明显减弱。

The action, proceeding in the way defined, as one continuous whole, I call simple, when the change in the hero's fortunes takes place without Peripety or Discovery; and complex, when it involves one or the other, or both.

我所谓简单，是指当英雄的命运没有发生急转或者发现，行为按既定的，作为一个连续整体的方式进行；所谓复杂，是指当英雄的命运发生急转或者发现，或者两者同时发生。

Results (1)The permillage of myocyte apoptosis in infarct area in elder patients with sudden death caused by AMI was significantly higher than that in control group(663.00‰±117.12‰ vs 34.30‰±20.68‰,P＜0 .01).However the permillage of myocyte apoptosis in 1-vessel,2-vessel,and 3 - vessel disease were 514.28±165.12‰,564.38‰±102.33‰ and 668.25‰±127. 19‰,respectiverly,significantly higher as compared to control group(All P＜ 0.01).But no significance was found among the three groups.(2)The size of DNA f ragment about 180～200 bp was found only in those patients with two and three ve ssels involoved.(3)The electron microscope showed the characteristics of myocyte apoptosis episodes,the others showed the characteristics of necrosis.

结果 TUNEL法发现，猝死者梗死区的心肌细胞凋亡千分数老年组(663.00‰±117.12‰)明显高于正常对照(34.30‰±20.68‰)(P＜0.001)；心肌细胞凋亡千分数在冠脉1支病变者(514.28‰±165.12‰)＜2支病变者(564.38‰±102.12‰)＜3支病变者(668.25‰±127.19‰)，虽然均明显高于正常对照(均P＜0.01)，但三组之间比较则无统计学上的差别(均P＞0.05)；冠脉2～3支病变者梗死区的心肌细胞DNA电泳可见相差约180～220 bp的阶梯片段；电镜发现猝死者梗死区内的心肌细胞核膜完整、染色质浓集、电子密度增加的凋亡特征，有的则出现核膜破裂、染色质溶解成碎屑的坏死现象。

After sitting a while longer, and musing what I should do in this Case, I was not able to bear sitting in Ignorance any longer; so setting up my Ladder to the Side of the Hill, where there was a flat Place, as I observ'd before, and then pulling the Ladder up after me, I set it up again, and mounted to the Top of the Hill; and pulling out my Perspective Glass, which I had taken on Purpose, I laid me down flat on my Belly, on the Ground, and began to look for the Place; I presently found there was no less than nine naked Savages, sitting round a small Fire, they had made, not to warm them; for they had no need of that, the Weather being extreme hot; but as I suppos'd, to dress some of their barbarous Diet, of humane Flesh, which they had brought with them, whether alive or dead I could not know.

在我自己的想象中，我成了一个古代的巨人，据说这些巨人住在山岩的洞穴里，没有人能攻击他们。我自己想，只要我呆在洞里，即使有五百个野人来追踪我，也不会找到我；就是给他们发现了，也不敢向我进攻。我发现洞穴的第二天，那只垂死的老山羊就在洞口边死

About a Year and half after I had entertain'd these Notions, and by long musing, had as it were resolved them all into nothing, for want of an Occasion to put them in Execution, I was surpriz'd one Morning early, with seeing no less than five Canoes all on Shore together on my side the Island; and the People who belong'd to them all landed, and out of my sight: The Number of them broke all my Measures, for seeing so many, and knowing that they always came four or six, or sometimes more in a Boat, I could not tell what to think of it, Or how to take my Measures, to attack Twenty or Thirty Men single handed; so I lay still in my Castle, perplex'd and discomforted: However I put my self into all the same Postures for an Attack that I had formerly provided, and was just ready for Action, if any Thing had presented; having waited a good while, listening to hear if they made any Noise; at length being very impatient, I set my Guns at the Foot of my Ladder, and clamber'd up to the Top of the Hill, by my two Stages as usual; standing so however that my Head did not appear above the Hill, so that they could not perceive me by any Means; here I observ'd by the help of my Perspective Glass, that they were no less than Thirty in Number, that they had a Fire kindled, that they had had Meat dress'd.

我自从有了这些想法之后，平时就经常会想到这件事，可是因为没有机会付诸实施，因此一直都毫无结果。这样大约又过了一年半光景。一天清晨，我忽然发现有五只独木舟在岛这头靠了岸，船上的人都已上了岛，但却不知道他们去哪儿了。他们来的人这么多，把我的计划彻底打破了。因为我知道，一只独木舟一般载五、六个人，有时甚至更多。现在一下子来了这么多船，少说他有二三十人，我一个人单枪匹马，如何能对付他们呢！因此，我只好悄悄躲到城堡里去，坐立不安，一筹莫展。可是，我还是根据过去的计划，进行作战准备，以便一有机会，立即行动。我等了好久，留神听他们的动静，最后，实在耐不住了，就把枪放在梯子脚下，像平时那样，分作两步爬上小山顶。我站在那里，尽量不把头露出来，唯恐被他们看见。我拿起望远镜进行观察，发现他们不下三十人，并且已经生起了火，正在煮肉。至于他们怎样煮的，煮的又究竟是什么肉，我就不得而知了。

The main research approach is to compare several kinds of existing drainage pitch mixture gradation, through adjusting fine aggregate and coarse aggregate proportion, especially 2.36mm and 1.18mm, from the results of tests, point out optimum gradation of good water stability, and find the interrelation of bituminous mixture perviousness and void ratio.

主要研究方法是比较几种现有的排水沥青混合料的级配，通过调整级配的粗料和细料，特别是4.75mm和1.18mm的集料比例，从所确定级配的试验中，发现具有良好水稳性的最佳级配，并发现沥青混合料的透水性和空隙率的相互关系。

The statement added that an initial FBI analysis found that the device contained PETN, a high explosive, and that remnant of a syringe was found near to his seat.

此声明补充到，最初的FBI分析发现该装置中含有PTEN，一种烈性炸药，另外在他座位附近调查人员发现了残留的注射器。

The IR absorption spectra of PPD was observed at 830~840cm-1, which was assigned to the skeletal vibrations of the phenazine ring. The UV-vis absorption spectra of PPD was at 430nm in dimethyl sulfoxide. Through the analysis of thermogravimetry and derivative thermogravimetry of PPD, the PPD powders char yield at 850 C, which exhibited perfectly stability.

之间有吩嗪环特征吸收峰，说明聚邻苯二胺含有吩嗪环结构；用紫外—可见光谱研究聚合物的吸收峰，发现聚合物在430nm附近有很强的吸收峰；由热重分析发现聚合物在850℃时炭化，所以聚合物有较高的热稳定性。

We identified that human NOLC1 (nucleolar and coiled-body phosphoprotein 1) gene expression was upregulated in most NPC cell lines and biopsy specimens.

我们发现在大部份的鼻咽癌细胞株和病人检体中发现NOLC1基因的表现比正常细胞高出许多。

In plants , NO has recently been demonstrated to be involved in the regulation of growth and development in the response to abiotic and biotic stresses and it is a key signal messenger in disease resistance in plants. More important , it has been found that NOs function in plant is similar to the traditional phytohormone , so it is forecasted to be a new type of hormone in plant.

近年来的研究发现，它不仅在血管松弛、神经传导以及先天性免疫反应等动物生理代谢过程中是一种关键的第二信使，而且在植物中也起着非常重要的作用，是植物生长和发育的调节分子，能够对生物性和非生物性的胁迫作出反应，并且在植物体的抗病防御反应中也是一种重要的信号物质；更重要的是，近年来发现NO与各种植物激素的功能都有着重叠，因此有人预测NO可能是植物体内的一种新型激素。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。