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Japonicus.Nerve extraction substance of sea star and dithiothreitol has been reported to be effectively to induce oocyte maturation in sea cucumbers. In this study we tested their effects on oocyte maturation induction in sea cucumber A. japonicus. The results are as follows, oocyte of A. japonicus does not get mature spontaneously. Neither NES alone nor NES with follicle suspension can induce oocyte maturation. While DTT can prominently increase the percentages of germinal vesicle break down. When the concentration of DTT is between10-1 mol/L and 10-3 mol/L, the effect of maturation induction is more sufficient. When the concentration is about 10-2 mol/L, the percentage of GVBD can reach up to more than 90%.

分别利用在海参中有诱导作用的海星神经提取液和二硫苏糖醇对从成熟卵巢解剖出的刺参卵母细胞进行诱导成熟试验,结果显示,刺参的卵母细胞在海水中不会发生自发成熟,海星神经提取液以及神经提取液加滤泡悬浮液对卵母细胞没有诱导成熟的作用,而二硫苏糖醇处理可以诱导卵母细胞生发泡破裂,当DTT溶液的浓度处于10-1 mol/L到10-3 mol/L之间时,对刺参卵母细胞的促熟作用较为明显,在10-2 mol/L左右时,卵母细胞的诱导成熟率可以达到90%以上。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle, GV)卵母细胞;用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达:其中cDNA的合成分两种方法进行:一是将GV期单个卵母细胞直接进行RT合成cDNA,二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT;回收产物构建克隆质粒并测序。

My company was founded in 1994, the existing PS foam extrusion line picture frame Article 5, have a professional mold production staff, welcomed the cooperation.

我公司成立于1994年,现有PS 发泡相框条挤出流水线5条,有专业的模具制作人员,欢迎合作。

In this study, we used poly L-latcide, poly DL-latcide-co-glycolide 75:25 (PLGA7525) and poly DL-latcide-co-glycolide 50:50 (PLGA5050) during citric acid inducing ammonium bicarbonate gas forming process to form porous polymer film, and rolled the porous polymer film to make nerve conduits with pores and multi-layered. Electron Spectroscopy for Chemical Analyzer and Attenuated Total Reflectance – Fourier-Transform Infrared Spectrometer were employed for determining elements' functionabilities and chemical compounds. Charge Coupled Device camera and Scanning Electron Microscope were employed for macroscopic and microscopic morphologies and structural observation. Differential Scanning Calorimetry was employed for measuring glass-transition temperature. Nano-indentation system was employed for measuring elastic modulus and hardness. Biodegradation and water absorption ratios were measured to analyze their chemical properties and SEM was employed for microscopic morphology of the tested nerve conduit.

本研究将以聚乳酸、聚乳酸-聚甘醇酸共聚合物(75:25)、聚乳酸-聚甘醇酸共聚合物(50:50)做为研究材料,以柠檬酸诱发碳酸氢铵发泡之气泡成型法制作具有相连通孔隙之高分子薄膜,再将高分子薄膜卷制成特殊多层的导管,制作具有多层孔隙之新型神经再生导管,以电子能谱化学分析及减弱式全反射-傅立叶转换红外线光谱分析进行成份分析及化学元素测定;以电荷耦合元件摄影机、扫瞄式电子显微镜进行多层孔隙型神经导管巨观及微观形态结构观察;以示差扫瞄热量量测分析玻璃转换温度、奈米三维量测弹性模数及硬度;以及进行降解性质测定,并以扫瞄式电子显微镜进行降解后之导管微观结构形态观察。

"" Mike Aitken winemaker at Woodgate said,there is a big growth opportunity for sparkling wine, it is our leader, it's where the market is heading.

伍德盖特葡萄酒厂的酿酒师麦克·艾特肯说:发泡酒有一个大的发展机会,这是我们的领路人,是葡萄酒市场的方向。

However, Steven van Hoeck, v.p. of sales at Alliance Gas Systems, believes the rough inner surface could be due to too much pressure on the gas or leaving the gas in the part too long—both causes for the gas to begin to act as a foaming agent.

然而,Alliance Gas Systems公司的副总裁Steven van Hoeck却认为内表面的粗糙是归因于气压太高或气体停留在制品内太久,因为这两个因素都使气体起到发泡剂的作用。

It has great bead and perfume of sweet pastries, golden delicious apple and a touch of lime.

此酒发泡好,有糕饼、苹果的香气和淡淡的酸橙香气。

While wines such as sparkling wines, chardonnay, pinot noir, sauvignon blanc and cabernets were featured I chose to focus on the benchmark sauvignon blanc.

展出的葡萄酒有发泡酒、夏顿埃、黑比诺、苏维浓白朗和卡本内,我主要品尝了具有指标意义的苏维浓白朗。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。