发明物

The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

The present invention relates to a coating composition comprising; A at least one polyester oligomer prepared from reactants comprising a 20 60 wt.% of at least one polyol, b 5 30 wt.% of at least one polycarboxylic acid selected from the group of cyclic polycarboxylic acids, the esters or the anhydrides thereof, wherein the carboxyl groups are separated by 3 carbon atoms or less, and from the group of alpha , beta -saturated acyclic polycarboxylic acids, the esters or the anhydrides thereof, and c 20 60 wt % of at least one monocarboxylic acid, the sum of the wt.% indicated for the reactants,, and always being 100 wt.%, and the oligomer being a low-viscosity oligomer having a weight average molecular weight Mw of less than 5,000, and a hydroxyl number in the range of about 200 to about 400 mg KOH/g oligomer, and B at least one polyisocyanate.

本发明涉及一种包含如下组分的涂料组合物：A至少一种由包含如下成分的反应物制备的聚酯低聚物：a20-60wt％至少一种多元醇，b5-30wt％至少一种选自其中羧基被3个或更少碳原子隔开的环状多元羧酸、其酯或酸酐以及选自α，β-饱和的无环多元羧酸、其酯或酸酐的多元羧酸，以及c20-60wt％至少一种一元羧酸，其中反应物、和的wt％之和总是为100wt％，所述低聚物是重均分子量Mw低于5000且羟基值为约200-约400mg KOH/g低聚物的低粘度低聚物，以及B至少一种多异氰酸酯。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤：(1)分别提取鱼类基因组DNA和线粒体DNA；(2)在鱼类线粒体DNA D-loop控制区设计简并引物：其上游引物MitD1-F有21个碱基：CAC CCY TRR CTC CCA AAG CYA；下游引物MitD1-R有23个碱基：GGT GCG GRK ACT TGC ATG TRT AA；(3)在引物的作用下进行PCR反应；(4)琼脂糖凝胶电泳；(5)对扩增的mtDNA D-loop基因片断进行测序。

Provided is a liquid crystal sealant composition in which an aqueous solution obtained by admixing the epoxy resin composition with purified water has an ionic conductivity of 1 mS/m or less; the B stage-reduced product has a viscosity of 50 to 10000 Pa.s at 80 to 100 DEG C; the cured matter of the above composition has a linear expansion coefficient of 10x10-5 mm/mm/ DEG C or less and a glass transition temperature Tg of 100 DEG C or higher; and the above cured matter has a moisture permeability of 200 g/m2.24 hours or less at 80 DEG C and which can meet a liquid crystal display element produced by a single layer press hot bonding system and exhibits high adhesion reliability and makes it possible to produce a homogeneous liquid crystal display element having a high quality.

本发明提供一种液晶密封剂组合物，其中：通过将环氧树脂组合物与相同质量的净化水混合得到的水溶液具有离子电导率为1mS/m或更小；在80～100℃下半溶阶段折合产物的粘度为50～10000Pas；上述组合物的固化物具有线膨胀系数为10×10－5mm/mm/℃或更小，热形变温度Tg为100℃或更高；且上述固化物在80℃时具有透湿率为200g/m224小时或更小，这能够满足通过单层热压粘结体系生产的液晶显示元件，并具有高的粘结可靠性，使得有可能生产出具有高质量的均匀液晶显示元件。

The invention is directed a patterned contact sheet and to a method of bonding a cover wafer to an interposer wafer using the patterned contact sheet having a waffle-like pattern of a plurality of ridges and plurality of wells to form a cover/interposer combination or unit that can be bonded to a substrate having a MEMs device thereon, the cover wafer, interposer wafer and substrate together forming a protective packaging for the MEMS.

本发明涉及在制造过程中保护临界表面的形成有图案的接触片材以及一种使用形成有图案的接触片材将盖子片粘合到插入物片上以形成盖子/插入物组合或单元的方法，所述接触片材具有华夫饼干状图案的许多脊和许多井，所述盖子/插入物组合或单元可粘合到具有MEM设备的基材上，所述盖子片、插入物片和基材一起形成MEM用的保护性封装。

The resin composition for a platable substrate of the present invention comprises 100 parts by weight of a resin composition comprising 10 to 60% by weight of a graft copolymer prepared by graft polymerization of a rubbery polymer (A1) and a monomer component (A2) containing an aromatic alkenyl compound monomer unit and a vinyl cyanide compound monomer unit and 40 to 90% by weight of the other polymer provided that the total amount of the components (A and is 100% by weight, and 5 to 40 parts by weight of a phosphate ester flame retardant having a molecular weight of more than 326 or 2 to 40 parts by weight of a red phosphorus flame retardant.

本发明涉及一种镀覆基材用树脂组合物，其特征在于，该树脂组合物是在100质量份的树脂组合物中，混合5～40质量份的分子量超过326的磷酸酯系阻燃剂或2～40质量份的红磷系阻燃剂形成的物质，其中树脂组合物由10～60质量％的接枝共聚物和40～90质量％的其它聚合物组成(A+＝100重量％，接枝共聚物通过在橡胶质聚合物(A1)上接枝聚合含有芳香族烯烃化合物单体单元和氰化乙烯基化合物单体单元的单体成分(A2)得到。

The compounds of the invention are also useful in the prevention and treatment of TRX-mediated diseases, such as autoimmune, allergic and inflammatory diseases, and in the prevention and/or treatment of diseases of the central nervous system, such as neurodegenerative diseases, The present invention further provides pharmaceutical compositions comprising the hydroxamic acid derivatives and safe dosing regimens of these pharmaceutical compositions, which are easy to follow, and which result in a therapeutically effective amount of the hydroxamic acid derivatives in vivo.

本发明的化合物还可用于预防和治疗TRX-介导的疾病，例如自身免疫性、变应性和炎性疾病，并且用于预防和/或治疗中枢神经系统疾病，例如神经变性性疾病。本发明还提供了包含所述异羟肟酸衍生物的药物组合物以及这些药物组合物的易于依从并且在体内产生治疗有效量的所述异羟肟酸衍生物的安全剂量方案。

The present invention further provides pharmaceutical compositions comprising the hydroxamic acid derivatives and safe dosing regimens of these pharmaceutical compositions, which are easy to follow, and which result in a therapeutically effective amount of the hydroxamic acid derivatives in vivo.

此外，本发明提供了药物组合物，其包含所述的异羟肟酸异羟肟酸衍生物，本发明还提供了这些药物组合物的安全的剂量给药方案，其可以容易地遵照，并且其在体内产生该异羟肟酸衍生物的治疗有效量。

The invention provides an asphalt thickener that comprises metal oxide and benzene ring –containing peroxidate and is 0.05~2% to basic asphalt mass.

本发明提供一种沥青增稠剂及沥青组合物。本发明沥青增稠剂包括金属化合物和含苯环的过氧化物。本发明沥青组合物中增稠剂为基质沥青重量的0.05～2％。

A method of dyeing a polyamide fiber structure characterized by using a polyamide fiber structure which has structural differences along the fiber length direction and dyeing the structure with an anionic reactive dye in a dye bath having a pH of 3 to 8; and a dyed polyamide fiber structure obtained by the above method.

本发明的聚酰胺类纤维构成物的染色方法，是以用纤维纵向上有结构差别的聚酰胺类纤维构成物，经将pH调至3～8的含阴离子性反应性染料的染液染色为特征的，本发明的染色物是由该染色方法制得的聚酰胺类纤维的染色物。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷