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The 824 bp fragment of exon 2 of melatonin receptor 1A(MTNR1A ) gene was amplified successfully by PCR in both 10 ewes of nonseasonal estrous breed and 10 ewes of seasonal estrous breed, and then cloned into pGEM-T Easy vector.

对常年发情的绵羊品种小尾寒羊和季节性发情的绵羊品种多赛特羊共20只母羊的褪黑激素受体1A(melatonin receptor 1A,MTNR1A)基因外显子2的824 bp扩增产物进行了克隆测序及序列比较分析。

Results showed that expression of ghrelin mRNA in the hypothalamus changed with the oestrous cycle, i.e., with the highest level in the proestrus and the lowest in the estrus.

结果表明下丘脑、垂体和卵巢ghrelin mRNA在母猪发情周期不同阶段都有表达,并且在发情周期不同阶段表达丰度有规律性变化。

All results above indicated that:The expression of ERβand p-ERβin mouse ovary at oestrous cycle and in early gestational period changed regularly, it showed that ERβand p-ERβhad important effects to ovaries development, ovarian follicles groth, oocyte maturation and form corepus luteum in female mouse ovary during oestrous cycle and in early gestational period.

以上结果表明:ERβ及其磷酸化形式——p-ERβ在小鼠发情周期以及妊娠早期卵巢中表达有规律性的变化,揭示ERβ与p-ERβ在雌性小鼠发情周期及妊娠早期,对卵巢发育、卵泡生长、卵母细胞成熟和黄体形成有重要的作用。

Effects on reproductive performance: The normal delivery of group B,C and D were 80%、93.33%、93.33%,respectively,were improved by a certain extent comparing with the control group; The mean birth weight of calves were not significantly different between the trial and control groups.But numerical datas shown that supplementing Zn-Met in dietary had the tendency to increase the birth weight of calves; Supplementing Zn-Met in dietary can make dairy cows express empathema earlier,and significantly(P.05).Among the trial groups,the advanced extent of the group C was comparatively large(5.8d);The oestrous cycles were all in normal range and not significantly different; The levels of serum PRL of group B,C and D were significantly(P.05) 1.46 ng/ml、3.08 ng/ml、3.45 ng/ml higher than the group A.

对繁殖性能的影响:添加蛋氨酸锌组的正常分娩率为80%、93.33%、93.33%,较对照组(73.33%)有一定程度的改善;试验组犊牛初生重与对照组犊牛初生重差异不显著(P>0.05),但数据结果显示,日粮添加蛋氨酸锌有使犊牛初生重增加的趋势;日粮添加蛋氨酸锌可使奶牛产后发情提前,且差异显著(P<0.05),其中,添加蛋氨酸锌240mg/kg组提前的幅度较大,提前了5.8d;试验组发情周期与对照组差异不显著(P>0.05),均在正常范围之内;B、C、D组血清促乳素含量均比对照组高,且差异显著(P<0.05),依次增加1.46ng/ml、3.08ng/ml、3.45ng/ml,试验组B与C、D两组间也达到显著差异(P<0.05)。

Vagina sponge bolt and CLO was used in oestrous synchronization of donors and acceptances , the rates of estrus was100.0% and 83.0%. 6mg FSH and CLO was used in superovulation of Hexi Fine wool goat,115 embryos were acquire totally and average14.4 embryos,100 useful embryos, average 12.5 embryos.The surgical operation used in embryos transplant, the rate of Gestation was 82.4%, 47 lambs was birth,and the birthrate of lamb was 167.9%.

采用阴道海绵栓结合氯前列稀醇法对供、受体进行同期发情处理,处理结果的发情率分别为100.0%和83.0%;采用 FSH剂量6m g/只结合氯前列稀醇对河西绒山羊超数排卵,共回收胚胎115枚,头均14.4枚,可用胚胎100枚,头均12.5枚;采用手术法对杂种河西绒山羊受体移植后,妊娠率82.4%,产羔47只,产羔率为167.9%。

The oestrus rate of ewes in high, middle and low nutrition level groups were 83.33%, 100.00% and 33.33% respectively.

上述结果表明,中等能量蛋白质营养水准能较好促进母羊的发情,缩短母羊产后的发情时间。

Estrus synchronization can be applied such as heat control technology, so that timely focus on oestrus ewes in a relatively short period of time breeding, pregnancy rate, subject to higher distribution, fully furnished full of school-age ewes, but also to improve the quality of the lamb.

同期发情,可如热控制技术的应用,以便及时对发情母羊集中在一个时间繁殖,怀孕率,受较高的分布,相对较短的时间齐全学校全年龄的母羊,而且提高羔羊的质量。

Diestrus: Lasting 60 to 90 days, diestrus is the period when the reproductive tract is under the control of the hormone progesterone.

发情间期:这段时期持续约 2-3 个月。发情间期,母犬生殖系统处于妊娠激素的调控之下。

The number of the medium sized follicles (diameter=3~5 mm) was highest (8 5/ovary) at diestrus and lowest (0/ovary) in metestrus.

中等卵泡数量在发情间期最多,为8.5个/卵巢,在发情后期卵巢中无中等卵泡。

Estrum of wild sow belongs to seasonality and often estrus in October to November.

母野猪发情有季节性,大约在每年的10~11月份;公野猪发情无季节性,可以常年配种。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。