发情

Result At the end of dioestrus, the estradiol level was increased gradually, and the peak of estradiol appeared from 1 day before oestrus to 3 days after oestrus (52.50±29.26) pg/mL, then the estradiol decreased rapidly, its lowest value at 6-9 days of oestrus (7.20±5.50) pg/mL. The level of progesterone was low before oestrus, and rose gradually at the peak of estradiol and reached the highest value at 9-15 days of oestrus (35.52±7.87) ng/mL, then it decreased gradually.

结果 发情间期的末期，雌二醇开始升高，在发情前1 d至发情开始后3 d内出现雌二醇高峰(52.50±29.26) pg/mL，峰值后雌二醇急剧下降，到发情第6~9天下降至最低值(7.20±5.50) pg/mL；孕酮水平在发情前期较低，于雌二醇高峰时开始上升，发情第9~15天达到最高值(35.52±7.87) ng/mL，此后逐渐下降。

In infundibulum and ampulla, the mPGES-2 staining from days 3 to 5 was very weak. During estrous cycle, mPGES-2 immunostaining in infundibulum was weak at diestrus and comparatively stronger at estrus. In ampulla, the immunostaining was nagtive in diestrus and weak in estrus.

发情周期中，mPGES-2 蛋白在发情间期伞部弱表达，在发情期伞部较强表达；在发情间期壶腹部不表达，在发情期壶腹部弱表达，与Western blot 检测整个输卵管的结果基本一致。

Methods Blood samples were collected from the precaval vein of 6 female Tibet mini-pigs to test the concentration of sex hormones including T,P4,E2,FSH,LH,PRL and blood biochemical indexes including Ca, Mg, P, ALP, Cre, CHO, TP, and Alb during the estrous cycle.

对6头雌性西藏小型猪在发情周期内的发情当天、第5天、第10天、第15天、再次发情时分别采血5 mL并分离血清，应用放射免疫法测定血清睾酮、孕酮(P4)、雌二醇(E2)、促卵泡生成激素、促黄体生成激素、泌乳素；用自动生化仪测定血清钙、镁、磷、碱性磷酸酶、肌苷、总胆固醇、总蛋白、白蛋白。

Methods: Adult mice of Kunming variety were used as experiment animals. After judgement of oestrus, the oestrus period and oestrus stage were determined, and then those mouse were grouped depending on oestrus stage. The mice were dealed with diferrent dosage of PMSG and HCG to make them superovulation, afterwords were mated individually with the male mouse together at same cage. The embryoes were rinsed out and evaluated after 3.5 days when the plug had been seen. The transplant mice were born after the high-quality embryoes were transferred into the receptors mouse uterus at the same oestrus stage which was stimulated by mating with the male mouse which spermaduct had been ligated previously.

以昆明系小白鼠为实验动物，取性成熟个体，先进行发情鉴定，确定其发情周期及不同的阶段，然后按发情阶段分组，分别用不同剂量的孕马血清促性腺激素和人绒毛膜促性腺激素进行超排处理并与公鼠合笼，在见栓后的第3.5天冲胚，对所得胚胎进行鉴定；将质量较好的胚胎选出，移入同期发情并经结扎公鼠交配刺激后见栓的受体小鼠子宫内，得到移植小鼠。

E. staining and other techniques were used to elucidate the pattern of follicular development, atresia, changes in follicle number on the ovarian surface, proportions of apoptotic granulosa cells in follicles of different sizes and morphological changes of atretic follicles, as well as changes in serum estradiel and progesterone levels at different stages of the porcine estrous cycle.

为探讨猪发情周期不同时期卵泡发育和闭锁的规律及机理，本实验通过TUNEL原位标记、H.E。染色以及放射免疫测定等手段研究了猪发情周期不同阶段卵巢表面各类卵泡数量的变化、各类卵泡中颗粒细胞的凋亡比例、闭锁卵泡的形态变化以及发情周期各阶段血清孕酮及雌二醇水平的变化等问题。

In the pituitary, the pattern of ghrelin mRNA expression during oestrous cycle markedly decreased in the estrus and metestrus. In the ovary, ghrelin mRNA exhibited with the highest level in the diestrus and the lowest in the proestrus, which was different from those in the hypothalamus and pituitary.

下丘脑ghrelin mRNA的表达在发情前期水平最高（p 0.05），发情期最低；在垂体，发情前期和间情期mRNA表达水平明显高于发情期和发情后期（p 0.01）；而卵巢中Ghrelin mRNA表达模式不同于下丘脑和垂体，在发情前期水平最低，然后逐渐升高，在间情期表达水平最高（p 0.01）。

The changing trend of progesterone during oestrous cycle resembles with that during the first 14d of early pregnancy. Its content reaches the peak value at lOd after oestrus and then declines. The estradiol peak appears at 3d of oestrous cycle, and the level rises again before oestrus. The concentration of LH declines after oestrus. The level of follicle stimulating hormone reaches the peak value at lOd after oestrus (2.40mIU/ml).

发情周期：民猪发情周期孕酮的含量与妊娠早期14天内的变化极其相似，在发情后第10天达峰值，而后开始下降；雌二醇水平在发情后第3天出现高峰，下次发情前开始升高；促黄体素在发情当天达最高水平，然后开始下降；促卵泡素在发情后的第10天达到峰值(2.40mIU/ml)。

The testing methods and targets were shown as below:Recorded milk weights from the 7th day postpartum to 15th day postpartum,and calculate the average day-milk yield;Milk samples were draw in 7th day and 14th day postpartum,and testing the milk fat,milk lactose,milk protein,milk Zn and the milk somatic cell counts;Blood samples were adopted at the 7th day postpartum,testing the levels of serum PRL; Blood samples adopted at the 1st day and the 12th day in oestrous cycle were used to test the levels of E_2 and P_4 in serum;In the experimental stage, registering the condition of parturition, the mean birth weight of calves,the first oestrus,the oestrus scycle postpartum and the conception rate of the first service after artificial insemination.

试验方法及所测指标如下：于产后第七天到第十四天记录日产奶量，最后计算每组平均日产奶量；每组随机抽取分娩后第七天、第十四天的奶样，测定乳糖、乳蛋白、乳脂、乳锌含量及乳体细胞数；于产后第七天，随机采取奶牛血样，测定血清中PRL含量；于发情周期的当天和第12天，随机采取血样，测定血清中雌二醇(Estradiol，E2)和孕酮(Progesterone，P)的浓度；记录奶牛的分娩情况、犊牛初生重、产后第一次发情日期、产后发情周期，并采用人工授精技术进行受精，记录第一情期受胎率，最后取平均值，作为衡量奶牛繁殖性能的指标。

Finally, the computer monitoring system was installed into a computer equipped with a data-collecting device. By using the system, the oestrus of dairy cattle could be predicted and forecasted timely and accurately.

将奶牛发情计算机监测系统安装于与数据采集设备相连的过程计算机中，对奶牛发情进行实时监测并做出及时准确的奶牛发情预报。

Before sexual seasons, because of induction estrus, the breeding of doe could be carried outahead of schedule and Pregnancy rate of doe was equal to Pregnancy rateof natural estrus doe.

在繁殖季节前期，诱导发情可以使母鹿提早配种，其受胎效果能够达到繁殖季节里自然发情受胎效果，可使母鹿发情集中和产羔提前。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失