发光菌属

Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群，序列同源性大于99%，与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6，6~18，18~66和66 h；培养12 h后，YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56，此后逐渐上升，至发酵结束时其pH值分别为7.74和8.07；培养0~18 h时2菌株发酵液中还原糖含量迅速降低，此后保持稳定；12 h时氨基氮含量达到最低，此后开始缓慢上升；YL001菌株培养54 h后及YL002菌株培养42和66 h后，其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。

TBA analysis showed 0.01% of TP had evident antioxidation on lipids from fish meat, and TBA increased with the TP concentration accretion presenting an unlinear relation. The effects of antioxidation of 0.03% and 0.07% of TP were close, thus, the best choice is 0.03% in practical application.5. 93 bacteria strains were isolated and categorized into several spoilage bacteria classes: Aeromonas, Vibrio, Pseudomonas, Enterobacteriaceae, Flavobacterium, Acinetobacter, Photobacteriaceae, Brevibacterium, and Micrococcus. In the early stage of storage (1～3 days), Gram-positive bacteria were the major strains and accounted for 70%, most of them belonged to Brevibacterium and Micrococcus.

TBA试验结果显示，添加00.1%以上茶多酚对草鱼脂肪都有明显的抗氧化作用，随浓度增加TBA值增加，但不呈线性关系，0.03%～0.07%抗氧化效果比较接近，从经济、有效的角度考虑在实际应用中可采用0.03%的茶多酚。5、从草鱼中分离出93株菌株，鉴定出以下几类主要腐败细菌：气单胞菌属、弧菌属、假单胞菌属、肠道杆菌科、黄杆菌属、邻单胞菌属、发光杆菌属、产碱杆菌属、短杆菌属、微球菌属、不动杆菌属、无色菌属。

Through the traditional physiological and biochemical method, together with the 16SrDNA sequence homology and phylogenetic analysis, the strains were identified. The result shows they belong to the genus photobacterium, but they differ from any species of this genus in some characteristic which suggests they might be new species, and their taxonomic placement needs more information on genes level.

对两株菌进行鉴定，分析其生理生化特征和16SrDNA产物序列，确定这两株菌均属于发光杆菌属，但是和该属的各种还有一定差异，对其分类地位的最后确定还需进一步的系统发育学分析。

Anguillarum isolates, while 94.3% and 91.9% nucleotide identical to L. pelagius and Photobacterium damselae respectively. MP analysis showed that ayu-H080701 shared 97.6%-98.8 % amino acid sequence identical to L. anguillarum isolates, while lower than 75.6 % to other bacteria. Phylogenetic analysis showed that ayu-H080701 grouped constantly with L. anguillarum isolates. The biochemical, physiological tests and sequence analysis all strongly supported the identification of the pathogen causing ayu vibriosis in Ninghai country, China, as an isolate of L.

PCR扩增检测表明，细菌16S rRNA 基因通用引物和鳗利斯顿氏菌MP基因特异引物均能扩增到预期大小的特异性条带。ayu-H080701与鳗利斯顿氏菌16S rRNA基因核苷酸序列同源性最高，为99.4%～99.5%，与同属的海弧菌和美人鱼发光杆菌分别为94.3%和91.9%；ayu-H080701与鳗利斯顿氏菌MP氨基酸序列同源性高达97.6%～98.8 %，与其它弧菌科成员则低于75.6 %，系统进化树分析也揭示ayu-H080701与鳗利斯顿氏菌进化相关性最高。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐