发光性的

The liqase was positively controlled by HAI and AI-2 to some extent. The lecithinase was negatively controlled by HAI-1, and the hemolysin was negatively controlled by both HAI-1 and AI-2. Special primers of luxS gene were designed from the luxS gene sequence of V. harveyi obtained from the GenBank, and the gene was amplified from a pathogenic strain V. harveyi VIB645 by PCR. The luxS gene was cloned to a cloning vector pUC, and was sequenced. It was found that the open reading frame is 519 bp. The similarity of the luxS gene from V. harveyi VIB645 to the published sequence of V. harveyi strain in GenBank was only 90%.

从GenBank中获取哈维氏弧菌中编码AI-2合成酶的luxS基因序列设计一对特异性引物从哈维氏弧菌VIB645中PCR扩增luxS基因，将luxS基因克隆于pUC载体并测序，发现其与GenBank中已发表的哈维氏弧菌的luxS基因序列相似性为90%，证明LuxS/AI-2系统在哈维氏弧菌不同的菌株中的具有一定的特异性，推测LuxS/AI-2系统可能与哈维氏弧菌的致病性或发光现象有关。

A photonic crystal-air interface or line defect is utilized as a holophote or a partial reflective mirror to design a paralleled plane cavity, a single ring cavity structure and a multi-ring cavity structure. The method has simple design idea, good integration and wide application; by adopting the method, the self aligning photonic crystal lasers with different wave bands can be designed according to the luminescent characteristics of different materials; particularly, the utilization of semiconductor materials to design a light source of optical communication wave bands has more important practical value.

利用光子晶体-空气界面或线缺陷作为全反射或部分反射镜设计平行平面腔、单环形和多环形腔结构；该方法设计思路简单，集成性好，适用范围广，可根据不同材料的发光特性设计不同波段的自准直光子晶体激光器，特别是用半导体材料设计光通讯波段的光源器件具有更重要的实用价值。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Moreover, the more extended and luminous revelation of Divine things, coupled with the greater abundance of spiritual assistance conferred chiefly through the instrumentality of the sacraments, make practice of virtue easier and more attractive at the same time more elevated, generous, intense and enduring, while the universality of Christianity lifts the practice of asceticism out of the narrow limitations of being the exclusive privilege of a single race into a common possession of all nations of the earth.

此外，更多的扩展和发光的启示神圣的东西，再加上更大的丰富的精神所赋予的援助，主要是通过工具的圣礼，实践以德治国更容易，更具吸引力的在同一时间，更多的升高，慷慨的，尖锐性和持久的，而普遍性的基督教升降机的做法，禁欲主义走出狭隘的限制，被排斥的特权，一个单一的种族纳入一个共同的藏有所有国家的地球。

During the process, LED is used as light source to raise image brightness and stability, images are taken from both the upper side and the lower side of the detected matter, and the detected matter is distinguished from two sides for raised distinction rate. In the foreign matter distinguishing and processing, the integral unit gray homogeneity is utilized in judging the unit attribute to distinguish the foreign matter with color similar to that of the detected matter.

在步骤(1)中，使用发光二极管作为光源，以提高图像的亮度和稳定性，并在待测物上下面都进行摄像，同时对待测物的两面进行识别，提高识别率；在步骤(3)中针对颜色与待测物相似的异物，利用单元整体灰度的均匀性来判别单元的属性。

The normally matte-black ablative coating on her dorsal surfaces flickered with patterns of cirrostratus and lapis ocean and glowing orange sunset.

正常的粗糙黑色烧蚀性涂层在它的外表面摇曳着卷层云、深蓝色的海洋和发光的橙色落日图案。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。