反转定向的

A pair of primers were designed based on M1 gene sequence of known H5N1 Avian influenza virus.M1 gene was cloned from total RNA,extracted from tissue of H5N1 subtype virus inoculated embryo by reverse transcriptase-polymerase chain reaction using high proofreading polymerase(Pyobest~TM DNA Polymerase),and expressed using Invitrogen champion~TM pET directional TOPO expression system.Recombinant protein containing polyhistidine(6xHis) tag in N-terminal about 29.8kDa in size,wac obtained and purified.

根据已发表的禽流感病毒M1基因序列设计合成PCR克隆引物，自接种H5N1亚型病毒的鸡胚组织中提取RNA，反转录后采用高可信度DNA聚合酶经PCR扩增M1基因，采用Invitrogen定向表达系统(ChampionTMpET directional TOPO expression system)进行克隆表达，纯化获得N末端携带多聚组氨酸标签的重组蛋白，分子量约29.8 kDa。

The parameters include waveguide width: 8μm, titanium film thickness:50~60nm, index change: 0.006, diffusion temperature: 1050 and diffusion time: 9~10 hours.Designed and produced a 2 X 2 coupler Optical switch and a 1 X 2 digital optical switch. The coupler optical switch was produced by AP reversal structure, with the main parameters such as total length of switch: 30mm, waveguides center distance: Hum, interaction length: 17mm, and at 17V with a crosstalk of -18dB. The digital optical switch was produced by asymmetry electrode Y branch structure. The main parameters of this switch such as switch length: 25mm, the angle of Y branch: 0=0.1? center distance at branch end: 30μm, the center distance of electrons: 12μm, and at 27V with a crosstalk of 18dB.

设计并制作了2×2耦合型光开关和1×2数字开关，其中耦合型开关主要采用的反转Δβ定向耦合器开关，开关长度约30mm，波导宽度8μm，波导间距14μm，耦合区长度17mm，电极主要采用三电极结构，最终测得对TE模开关电压＜-15，消光比≈18dB；数字开关采用非对称电极Y分叉数子开关结构，利用BPM软件模拟了其开关特性并优化了器件参数，制作中采用的主要参数为：开关长度约25mm，Y分叉角θ=0.1°，Y分叉末端波导间距30μm，电极间距12μm，最终测得开关电压27V，消光比≈18dB。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失