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METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组,在实验的24, 48和72 h提取细胞总RNA,进行反转录PCR,检测整合素的α5,αv和β1亚单位mRNA表达,并计算与内参照β肌动蛋白 beta????

METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits. Each value was normalized against that of β actin mRNA.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组,在实验的24, 48和72 h提取细胞总RNA,进行反转录PCR,检测整合素的α5,αv和β1亚单位mRNA表达,并计算与内参照β肌动蛋白 mRNA水平的比值。

The first column of the inverse of M1 is the required right vector, and the second column represents the up required vector.

反转M1矩阵后的第一列是我们需要的右向量,第二列是我们需要的上向量。

His sorrow shall be turned on his own head: and his iniquity shall comedown upon his crown.

他的凶恶必反转到自己头上,他的横暴必降落在自己的顶上。

The logic inversion of rock compressibility has been overcome by a new measurement method.

岩石压缩系数的逻辑反转现象,已通过新的实验方法加以克服。

The conjectural restoration of the order of the chapters should be made, according to Ryssel, on the basis of manuscript No.

该推测恢复秩序的章节应根据Ryssel的基础上,手稿第248号,这也避免这一反转

When content of COPEI resin is above 30 wt%, utter phase dispersions occur, furthermore with increasing of contents of C...

当COPEI树脂含量在30%以上时,共混体系出现完全反转相结构,随着其含量增加,环氧树脂分散相尺寸逐渐减小,制得的环氧树脂微球粒径和粒度分布逐渐降低。

E. , the competition between the n - p interaction and the coriolis force in low K space is also appropriate for odd-odd nuclei in the A = 100 region.

迄今为止,对于奇奇核的旋称反转机制到底是什么和是否存在一种适用于各个不同质量区的普遍机制仍没形成共识。

To do that, I would have to move the planets counterclockwise.

为了做到这一点我必须反转星盘,这意味这至少向西移动40度,最后我去了L.A。

To do that, I would have to move the planets counterclockwise. That meant traveling at least 40 degrees west. Going to L.A.

为了做到这一点我必须反转星盘,这意味这至少向西移动40度,最后我去了L.A。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。