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Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,ChinaAbstract: Nine samples from swine abortus were detected by RT-PCR with a pair of primers specific to NS1 gene of Japanese encephalitis virus.

采用反转录聚合酶链式反应方法检测猪流产胎儿病料中的乙型脑炎病毒,并对阳性病料做病毒分离与纯化。

Equal amount of absolute alcohol was given to the control group. The expressions of vitamin D receptor in HL-60 cells of each group were identified by Western blot analysis, and cellular morphological changes were observed after being stained by Wright-Giemsa.

Western印迹技术鉴定维生素D受体在各组HL-60细胞的表达;Wright-Giemsa染色观察细胞形态学变化;流式细胞术检测细胞表面标志物CDl4抗原的表达;反转录-聚合酶链反应法分析c-myc基因的表达。

Both a reversion and an increase of the absolute value of specific rotation in the transformation from MALM to PMALM- b -PSF- b -PMALM were observed,which were probably caused by some kind of stereostructure of the main chain formed during the polymerization due to the intermolecular hydrogen-bonding.

共聚物在聚合过程中旋光度发生了反转,且比单体的旋光度的绝对值显著增加,这可能是由于聚合过程中分子主链感应出了手性的二级结构诱导出手性放大效应引起的。

In order to construct a normalized cDNA silencing library, a novel procedure was adopted to reduce the abundant copy number and increase the rare copy number. Then the normalized cDNA was put into pTRV vector to construct a cDNA library and this cDNA library was screened with the technology of virus induced gene silencing.

然后把均一化之后mRNA反转录得到cDNA,再与基因沉默载体pTRV重组,最后把构建好的载体通过电转化的方法转入到GV3101农杆菌中,从而建立起破色期番茄果实均一化cDNA沉默文库。

The results showed that the method of rubbing the kerf then injection of retrovirus suspension into pancreas could more effectively mark the pancreatic cells than the caudal vein injection and the intraperitoneal injection did in vivo. Furthermore, this study also found that some acinus cells could accept injury stimulus signals to regenerate through resuming mitosis after pancreatic injury.

结果发现在活体条件下,与尾静脉注射及腹腔注射法相比,胰腺伤口涂抹并胰腺内注射反转录病毒的方法能够更有效的标记胰腺细胞;而且,通过对标记细胞的世系追踪研究证明,在胰腺损伤后,胰腺腺泡细胞能够接受损伤信号刺激发生再生。

Vertical radiation diagram of Inverted Amos antenna with different number of 3, 5, 7, and 9 dipoles.

图 12: 3,5,7,9 振子反转阿摩司天线垂直方向图 9 July,2009 反转阿摩司天线 Fig。

The sequence of solutes flowing from the chromatogram would be changed at the inversion point, and so would the entropy condition.

反转点处,对应的吸附能力相邻的两种溶质的分离顺序发生反转,熵条件也随之改变。

As you scan futures markets for inversions, keep in mind that there is one area in which inversion is the norm.

当你扫描期货市场找反转时,记住,在有些地方出现反转是正常的。

It can also restrict other retroviruses, therefore, suggested as a general defender against retrovirus infection.

TRIM5α也能抑制其它的反转录病毒,因此可能具有广普的抗反转录病毒感染的作用。

In the fast reversal, you use primarily aileron to roll with…in the high g reversal, you use a combination of aileron and rudder to roll with.

前一种反转主要利用副翼滚转,后一种则同时利用副翼和舵来完成反转

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。