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We use the kidney to research the issue of that cystamine can regulate kidney to improve the disease or not. In flow cytometry, we use CD8/CD4/CD25 makers to observe the balance of TH1/TH2, and we find that cystamine can raise TH2 expression to produce autoantibodies and also can induce overexpression of Treg to ease inflammation.

为了同时在免疫方面观察胱胺是否也有著能减缓SLE的倾向,进而深入了解观察肾脏这个重要的次级免疫器官,探讨cystamine是否对於肾脏也具有重要的调节作用进而能改善病症,在本实验中以flow cytometry对免疫倾向的确认,透过CD8/CD4/CD25 maker观察TH1/TH2 balance,发现胱胺能有效的反转SLE大量倾向TH2进而产生的自体抗体,同时也能增加 Treg的高量表达进而去抑制免疫反应缓解发炎。

The morphological and cytopathological characters of the viruses were observed by electron microscopy The types of virus were identified with I-ELISA, DAS-ELISA, TAS-ELISA, RT-PCR and IC-PCR. The CP genes of the main viruses identified in this research were amplified by PCR, cloned into pGEM-T and sequenced. The pumpkin germplasm from Heilongjiang and Yunnan provinces was screened for resistance to the main viruses using friction and roots immersed inoculation.

应用电子显微镜负染法和超薄切片法观察病原粒体形态及细胞病理学特征;利用酶联免疫吸附测定法中的双抗体夹心ELISA、间接ELISA、三抗体夹心ELISA以及反转录聚合酶链式反应、免疫捕捉PCR方法鉴定了采集样品中的病毒种类;对被确定为南瓜主要病毒病原的外壳蛋白基因进行了PCR扩增,克隆到pGEM-T载体并进行测序;应用摩擦接种法和浸根接种法对云南、黑龙江省部分南瓜品系、品种进行了抗性筛选。

In sham-operation group,animals were decollated and their brains were taken out at 24 hours postoperatively. In all the groups, hippocampal brain injury was detected using histopathological method combined with semi-quantified RT-PCR methods for detecting caspase-3 mRNA. Adult rats aged 90 days were used in modified y maze to examine their learning and memory functions. All these three experiments were independent.

各组海马区脑损伤行组织病理学检测,半胱氨酸天冬氨酸蛋白酶-3 mRNA的表达采用半定量反转录-聚合酶反应方法进行检查,成年90 d龄的大鼠进行三等分迷宫测试其学习记忆能力,3个实验各自独立。

A total of 90 healthy male Sprague Dawley rats were selected for this study. Twelve rats were used for the sham operation group. Left anterior descending coronary artery of the remaining rats was deligated to establish models of myocardial infarction. After 24 hours of deligation, 36 rats were randomly divided into the control group and experimental group, 18 rat in each group.

目的:检测人胰岛素样生长因子Ⅰ基因修饰的鼠骨骼肌成肌细胞移植后,心肌梗死大鼠血浆及心肌组织内胰岛素样生长因子Ⅰ蛋白浓度的变化,以及心肌细胞内胰岛素样生长因子Ⅰ和端粒酶反转录酶mRNA水平的表达。

Cation group has the capability of charge neutrality and this would make oily wastewater demulsify and remove oil easily. Hydrophobic group would improve hydrophobic-oleophilic nature of the oil granule. Simultaneously, wetting reversed agent would change wetting performance of oil granule, enhance conglutination capability between air bubble and oil granule. And Frother would form bigger foam layer on the water, so it could prevnt granule with air bubble from rolling downward.Using the hydrophobic polymeric flocculant as one of ingredients for flotation agent formula, the flotation agent has good treatment effect.

浮选剂配方中的疏水性高分子絮凝剂分子链上带有的阳离子基团起到电中和作用,使含油污水易于破乳除油,而疏水基团能增强颗粒的疏水性能;润湿反转剂能改变颗粒的润湿性,增强气泡与颗粒的粘附能力;起泡剂在水面能形成强度较大的泡沫层,起阻止带气絮粒向下翻动的作用。

On the basis of DDRT-PCR technique we found that:Total of 331 differential displayed cDNA bands from 1161 were displayed in the 4x4 diallel cross combinations with 30 pairs of primers, which shows the differences of gene expression between hybrids and their parental lines were very obvious in quantity and quality.

采用mRNA差异显示技术和荧光定量反转录PCR技术,从随机mRNA差异显示和候选功能基因mRNA表达定量分析两个方面,研究了32周龄杂种鸡及其亲本纯种的基因差异表达及其与繁殖性状杂种优势的关系,探讨了鸡繁殖性状杂种优势形成的因素及其分子遗传机理。

Germ-line mutations in the genes hTERT and hTR, encoding telomerase reverse transcriptase and telomerase RNA, respectively, cause autosomal dominant dyskeratosis congenita, a rare hereditary disorder associated with premature death from aplastic anemia and pulmonary fibrosis.

分别编码端粒末端转移酶反转录酶和端粒酶RNA的hTERT和hTR基因的种系突变导致常染色体显性先天性角化不良,它是一种罕见的因为再生障碍性贫血和肺纤维化而早亡的遗传性疾病。

It is also found that the electroosmotic flow is in the opposite direction to that predicted by the classical continuum theory.

模拟结果表明,在双电层的某个区域内,同号离子的浓度超过了对离子的浓度,出现了电荷倒置现象;约占整个通道的三分之二内的区域内,溶液朝着与连续性理论预测的相反方向流动,出现了反转电渗流。

Logging truck is a kind of special equipment used widely in well logging and perforation. It sends logging instrument to different depth of well via the rotation of roller with cable enwound on it.

测井车是油田行业用于测井及射孔的专用设备,该设备通过缠绕电缆的滚筒的正反转将测井仪器放入井下或提回井面,以测得油井不同深度的各种性能参数。

In this paper, the model is limited in a nearly equispaced Ladder-type system, we explained the effects of spontaneously induced coherence on the optical properties, such as population inversion and absorption, and so on.

本文探讨了自发辐射诱导相干对近等间隔的阶梯型三能级系统的稳态几率反转及吸收等光学特性的影响。

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