反相

OBJECTIVE:To establish the RP-HPLC method for determination of methyl hydroxybenzoate and ethyl hydroxybenzoate in oral liquid preparations METHODS:The Nova-Park C18 column(3 9mm×150mm,5μm)was used The mobile phase consisted of acetonitrile-water(25∶75)at a flow rate of 1ml/min,the detection wavelength was set at 254nm and the sensitivity was set at 0 01 AUFS RESULTS:The linear ranges of methyl hydroxybenzoate and ethyl hydroxybenzoate were 0 4 412～13 2 360μg/ml(r=0 9 999) and 0 6 672～20 0 160μg/m...

目的 ：建立内服液体制剂中羟苯甲酯和羟苯乙酯的反相高效液相色谱测定方法。方法：色谱柱：Nova -ParkC18 柱(3 9mm×150mm ，5μm)；流动相：乙腈-水(25∶75)；流速：1ml/min ；检测波长：254nm ；灵敏度：0 01AUFS。结果：羟苯甲酯浓度在0 4412～13 2360μg/ml范围内线性关系良好(r=0 9999)；羟苯乙酯浓度在0 6672～20 0160μg/ml范围内线性关系良好(r=0 9998)。结论：该方法简便、快速、准确，可用于医院制剂中防腐剂的检测。

Department of Chemical Pharmacology, Capital University of Medical Science, Beijing 100054, ChinaAbstract: A reversedphase high performance liquid chromatographic method was developed for the simultaneous quantitative determination of caffeine, dapsone and chlorzoxazone. The operation was carried out on a C18 column (250 mmX4.6 mm i.d., 5.0 μm) with the mobile phase of acetonitrilephosphate buffer including 0.02 mol/L KH2PO4 and 0.02 mol/L (C2H53N, pH 6.5(25:75 in volume ratio).

用反相高效液相色谱同时测定血清中咖啡因、氨苯砜、氯唑沙宗探针药物的质量浓度，以乙腈磷酸盐缓冲体系(含 0.02 mol/L的磷酸二氢钾和0.02 mol/L的三乙胺，pH 6.5)(体积比为25：75)为流动相，以安替比林为内标，经C18柱(250 mmX4.6 mm i.d。， 5.0 μm)分离，紫外检测器检测，使3种探针药物得到较好的分离，并且在有效血药浓度范围内线性良好。

Utilizing the reversed-phase high performance liquid chromatogram to carry out further purification to the deer placenta polypeptide, the best purifying condition of separation is: Chromatogram column Hypersil ODS C18 (4.6*250mm), A liquid 0.1% TFA /ACN, B liquid 0.1% TFA / water, in 30min10% A ～50% A and 90% B ～50% B linear gradient elutes, velocity of flow 1.0mL/min, temperature 30℃, detecting wavelength 214nm.

利用反相液相色谱技术对鹿胎盘多肽进一步纯化，最佳分离纯化条件为：色谱柱 Hypersil ODS C18(4.6×250mm )，流动相 A 液0.1% TFA/乙睛，B 液0.1%TFA/水，30min 内10%A ～50%A ，90%B ～50%B 线性梯度洗脱，流速1.0mL/min ，温度30℃，检测波长214nm 。

We use AOT/iso-octane/water reverse micelles as a biological model to mimic the properties of triplet-excited-state porphyrin in the interfacial region. The amphiphilic property of compound is difficult to evaluate for different system.

用AOT／异辛烷／水反相胶束体系营造了一个多相、多界面的环境，通过对卟啉化合物三重态寿命的测量，研究了以亲水基团为端基的具有不同侧链链长的两亲卟啉化合物的界面行为，探讨了卟啉化合物的亲水疏水性质与界面环境间的相互作用，这对卟啉类光疗药物的结构优化具有帮助。

The XLR-type jack is wired pin 2 HOT, pin 3 COLD and pin 1 GROUND.

XLR类型插头的2脚为同相端，3脚为反相端，1脚是地。

The XLR-type jack is wired pin 2 HOT, pin 3 COLD and pin 1 GROUND.

XLR型接口的2脚接同相端，3脚接反相端，1脚接地。

A sensitive HPLC method for the simultaneous determination of diniconazole, thiram and imidacloprid from the mixture was developed.

采用反相高效液相色谱法，使用YWGC18柱和二极管阵列检测器，以甲醇-水为流动相，检测波长260nm，用外标法测定了福美双·吡虫啉·烯唑醇悬浮种衣剂中3种成分的含量。

A sensitive HPLC method for the simultaneous determination of diniconazole, thiram and imidacloprid from the mixture was developed. YWG C18 column saved as solid phase and CH3OH-H2O as mobile phase. Detection wavelength was set at 260 nm and quantitative analyzing was performed with an external standard method.

采用反相高效液相色谱法，使用YWG C18柱和二极管阵列检测器，以甲醇－水为流动相，检测波长260 nm，用外标法测定了福美双吡虫啉烯唑醇悬浮种衣剂中3种成分的含量。

Objective To establish HPLC-UV method for determination of glycyrrhizin acid in compound nasal spray of glycyrrhizin and flower flos magnoliae,the chief active ingredient of formulation,glycyrrhizin acid,and to observethe therapeutic effect of this formulation on guinea pig's hypersensitive rhinitis.Methods A compound nasal spray formulation was prepared using extracts of glycyrrhizin and measured by HPLC-UV method.Mobile phase conˉsisted of methanol,0.2mol/L ammonium acetate and glacial acetic acid（67:33:1,v/v）,analytical column was Zorbax ODS C 18 and wavelength250nm.

目的 建立测定复方甘草和辛夷喷雾剂含量的方法并观察该制剂对豚鼠超敏性鼻炎的疗效方法以甘草提取的甘草甜素和辛夷花蒸馏提取液主药制备成鼻用喷雾剂，用反相高效液相色谱法测定制剂中甘草酸含量，检测波长为250nm，Zorbax ODS C 18 分析柱，流动相为甲醇：0.2mol/L醋酸铵：冰醋酸（67：33：1）。

A HPLC method for determination of sorbic acid,benzoic acid and sodium saccharin in dairy drink was studied.The comparison of five methods of precipitating protein showed that the solution of potassium ferrocyanide and zinc sulfate as protein's precipitant was appropriate.

探讨含乳饮料中山梨酸、苯甲酸、糖精钠的HPLC含量测定方法，在比较5种沉淀样品中蛋白质的方法后，确定以亚铁氰化钾与硫酸锌溶液为沉淀剂，采用C18反相柱，以甲醇：乙酸铵溶液(0.02 mol/L)=15∶85为流动相，紫外230 nm检测，结合保留时间及二极管阵列检测器光谱分析信息定性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。