反相

Keywords: diketoreductase,isomeride,separation and purification,statins chiral side-chain expression, photoaffinity labeling probe

通过DEAE-Sepharose离子交换柱及Sephadex分子筛柱纯化后，酶的纯度达到电泳纯，并通过正相硅胶柱和反相C-18柱分离纯化其反应产物。

A method for the separation and determination of Pueraria lobata isoflavonoid extract by reversed-phase high performance liquid chromatography was proposed.

采用反相高效液相色谱法，在C1 8柱上以甲醇水为流动相进行梯度洗脱，在 0～ 1 0min内将葛根黄酮提取物进行分离，并在 2 5 0nm检测，采用标准加入法对实际样品中的葛根素含量进行了分析。

RP-HPLC is employed to determine the Isoflavones, with a C18 RP Col umn, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm.

反向高效液相色谱法进行含量测定，C18反相柱，MeOH CH3CN 0.1%H3PO4 为流动相，检测波长 260nm。

C18 esterified silica monolithic column emerging from chromatogram realm was made by use of solid-phase extraction medium material in μ-TAS. Several simple solid-phase extraction pretreatment units were set up to preconcentrate dobutamine hydrochloride which was detected by dobutamine hydrochloride-KMnO4-Luminol chemiluminescence system.

以改性C18反相硅胶整体柱为微流控芯片分析系统中固相萃取介质材料，构建不同功能单元的样品预处理微分析系统，实现了血清样品中痕量盐酸多巴酚丁胺的富集。

Liposomal entrapment efficiency of urokinasein by MREV is as high as 65％, which is not evidently influenced by incorporation of phospholipid derivatives such as DSPE-PEG-RGDS and Stearyl RGDS.

改良反相蒸发法在磷脂相转变温度以下制备脂质体以提高包封率的效应在尿激酶上得到了很好的体现。

Meanwhile, the steric effects of alcohol seemed to be a certain factor.

对托品酸乙酯在CTB手性固定相上反相手性识别机理进行了讨论。

The structures of the triterpene glycosides were elucidated based on spectral data, chemical reactions, and related references.

利用大孔树脂柱层析、正相硅胶柱层析、反相硅胶柱层析、Sephadex LH-20以及HPLC等层析手段，对一种辐肛参的皂苷类成分进行分离，应用波谱技术和化学方法，并结合文献对照，对所得的化合物进行结构鉴定。

BQJ-34 was cultured on the biotin fermentation media for 48 hour ,a 10ml fluid medium was boiled for an hour and was centrifuged (10,000r/min , 30 min), the supernatant is filleted with the 0.22μm filters and the biotin content of filter liquid is detected by RP-HPLC based on the biological samples regression analysis, recovery, accuracy testing.

利用生物素培养基对菌株BQJ-34进行振荡培养，取48h的细菌培养液10ml置100℃条件下水浴1 h，随后在超速离心机上以10 000 r/min，30 min离心，取上清液用0.22μm滤膜过滤，用高效液相检测滤液中的生物素含量，并通过对生物素样品的回归分析和回收率、精密度试验，最终确定利用反相高效液相色谱法可用于培养液中生物素测定，其最佳检测条件是：用安捷伦Zorbax SB-C18(4.6~※250cm，5μm)色谱柱，以K_2HPO_4(0.04mol，pH2.5)和CH_3CN(二者体积比为91.5：8.5)为流动相，紫外检测波长为215nm。

The mobile phase was 0.1 mol/L ammonium oxalate + N,N-dimethylformamide + 0.2 mol/L diammonium phosphate (75+15+10, by vol) adjusted to pH 8 by aqueous ammonia. Column temperature was 30℃, flow rate was 0.8 ml/min, and detection wavelength was 365 nm.

使用C18反相柱和二极管阵列检测器，以0.1mol/L草酸铵溶液+二甲基甲酰胺+0.2mol/L磷酸氢二铵溶液=75+15+10(用氨水调pH值至8.0左右)为流动相，柱温30℃，流速0.8ml/min，检测波长365nm，外标法对水合霉素的有效成分进行定量分析。

The results suggested that the reaction temperature, the ratio of oil to water, the amount of ethyl cellulose as dispersant, initial monomers composition, the drop manner and the amount of initiator, the stirring speed, the pH of the buffer and the elution process have great influence on the morphology such as diameter of the hydrogel beads.

研究结果表明，采用反相悬浮聚合法，以丙烯酰胺和N-乙烯基吡咯烷酮为共聚单体，N，N'-亚甲基双丙烯酰胺为交联剂，牛血清白蛋白为模板，可以制备BSA印迹共聚物微球；反应温度、水油比、乙基纤维素分散剂用量、初始共聚单体配比、引发剂用量和加入方式、搅拌速度、水相pH值以及后续洗脱条件等均对粒径等微球结构和形态产生明显影响。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了