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Methods: Antineoplastic compounds were screened by bioassay-guided method with partitive extraction and multiple column chromatographies(Sephadex LH-20, ODS and preparative HPLC). Structures of active compounds were determined by high-resolution 2D NMR(600 MHz) and ESI-MS techniques.

通过分级萃取和多种硅胶柱层析(Sephadex LH-20凝胶过滤、ODS反相层析和HPLC)追踪分离抗癌活性成分;利用电喷雾离子质谱和高分辨(600 MHz)核磁共振二维谱技术鉴定化合物的结构。

Methods: Antineoplastic compounds were screened by bioassay-guided method with partitive extraction and multiple column chromatographies(Sephadex LH-20,ODS and preparative HPLC).

通过分级萃取和多种硅胶柱层析(Sephadex LH-20凝胶过滤、ODS反相层析和HPLC)追踪分离抗癌活性成分;利用电喷雾离子质谱和高分辨(600 MHz)核磁共振二维谱技术鉴定化合物的结构。

Characterization of CGRP in perfusate showed one predominant peak to coelute with synthetic rat CGRP by reverse-phrase HPLC.

反相高压液相分析,内毒素灌流所得馏分的CGRP免疫活性峰位在第40min,与大鼠CGRP标准品的峰位吻合。

Methods according to the national pharmacopoeia(2000), determinate the content of moist and ash.

按照2000版《中华人民共和国药典》的方法进行药材水分及灰分测定;运用改良的反相高效液相色谱的方法,对薯蓣皂苷元进行测定。

The dramatic movie demonstrates that ring particle density and reflectivity makes some parts of the shadowed side nearly the photographic negative of the sunlit side, but nearly empty regions remain continually dark.

由这精采的影片看得出,由於环上粒子的密度和反射率不同,有些地方在向阳面和背阳面看起来是完全反相的,但几乎没有东西的地区看起来则一直都是黑的。

Methods The rabbit model with hyperhomocysteinemia was established by L-methionine subcutaneouse injection. The changes of white microthrombus and microvascular diameter in pia mater microvessel were observed by using cranial window method and closed microcirculation detection system, The concentration of Hcy was detected by high performance liquid, The level of von Willebrand factor, endothelin-1 (ET-1) were assayed by ELISA. The content of NO was measured by nitrate reduction method. Meanwhile, the relationship between the changes of microvascular diameter and blood concentration ET-1, NO was analyzed.

采用L-蛋氨酸皮下注射法建立HHcy家兔模型,应用"颅骨窗口法"和微循环闭路检测系统观察其软脑膜微血管中白微栓数量及微血管口径变化;采用柱前衍生反相高效液相色谱-荧光法检测同型半胱氨酸浓度;平行观测血管内皮功能指标血管性血友病因子抗原(vWF,用酶联免疫吸附法)、血浆内皮素-1ET-1,用ELISA)、血清一氧化氮水平;并对HHcy所致ET-1、NO血液浓度变化和微血管口径变化进行相关分析。

Objective: To develop a RP-HPLC method for determination of three alkaloids in total alkaloids exfracted from Lotus plumule .

中文摘要:目的:建立同时测定莲子心中莲心碱、异莲心碱、甲基莲心碱含量的反相高效液相色谱法。

8 Kinds of food additives, tartrazine, amaranth, benzoic acid, saccharine, sorbic acid, ponceau 4R, sunset yellow FCF and brilliant blue FCF in fruit juices were simultaneously determined by reversed phase-high performance liquid chromatography on a Hypersil BDS C18 column5 μm, 250 mm×4 mm i.

建立了反相高效液相色谱法同时直接测定进口果汁中柠檬黄和苋菜红等8种添加剂含量的方法。

Results showed that the inhibition type of ACE was competitive.Finally, we make componentⅡthrough further purity by using DEAE Anion exchange column and Sephadex LH-20. After that com- ponentⅡwas separated to two parts.

六、将粗分物组分Ⅱ用DEAE阴离子交换柱和SephadexLH-20继续分离纯化,最终得到两个组分,用反相高效液相色谱C18柱检测其纯度。

Objective: To develop a HPLC method for the determination of quercetin in Quercetin capsule .

目的 :建立反相高效液相色谱法检测槲皮素胶囊中槲皮素含量方法。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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