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We further evaluated the synergistic cytotoxicity of TRAIL in combination with actinomycin D, cisplatin and paclitaxel in A549 cell lines in vitro and in vivo.

本实验选择了人肺腺癌A549细胞瘤株作为我们实验研究的对象,通过TRAIL对人肺腺癌A549细胞诱导凋亡研究,在A549的荷瘤裸鼠的动物模型上研究了其体内的抗肿瘤活性,并进一步研究了TRAIL与化疗药物放线菌素D(actinomycin D,Act D)、紫杉醇和顺铂(cisplatin,CDDP)的联合作用对人肺腺癌A549细胞的体外协同抗肿瘤活性,通过化疗药物协同增强TRAIL抗肿瘤活性作用机制的初步研究,为降低临床化疗药的用药量,减轻其对机体的不良反应提供一定的理论基础。

The action spectrum analysis shows that the structure of daidzein-phospholipid complex is different from the reactant monomers and their physical mixture.The complex is a new product that is significantly different from those of daidzein.

对制备出的复合物进行紫外光谱、红外光谱、差热和X-衍射等光谱学分析,结果表明,大豆黄素与磷脂形成了复合物,且复合物是不同于反应物单体及单体物理混合物的新物相。

In some cell lines there is a similar periodicity in changes of cell surface antigenicity or response to wheat germ agglutinin.

在某些细胞系中,细胞表面抗原性或对表胚凝集素的反应方面有相近似的周期性变化。

Objective This study was aimed at optimizing the preparation of 5-Fluorouracil and peanut agglutinin conjugates so as to obtain a conjugate with high activity and high drug ratio, and to choose a suitable dosage form in the light of its properties.

目的 优化5-氟尿嘧啶(5-Fu)-花生凝集素结合物的反应条件,以期制备得到既有生物活性又有较高药物结合率的结合物。

Polymerase chain reaction ; agglutinin ; pneumonia ; mycoplasma

聚合酶链反应;凝集素;肺炎;支原体属

Department of Basic Medical Sciences,College of Vocational Technology,Liaoning University of TCM,Shenyang 110101.China Objective To observe immune regulation effect of Liyankang granule on children.Methods The present study has been carried out using NaCMC injected into the mice's abdominal cavity to cause inflammation and induce white blood cell migration,varnishing DNFB to induce mice's DTH,injecting colloid activated carbon suspension into mice's tail vein,with 10% of sheep RBC injected into the abdomen to immunize mice.We observed inhibitory function of Liyankang granule on NaCMC to cause inflammation and induce white blood cell migration,the effect of DNFB on inducing mice's DTH and the effect on clearance speed of carbon particle in mice's blood and serum agglution of mice.

采用大鼠腹腔注射羟甲基纤维素钠致炎诱发白细胞游走,涂抹二硝基氟苯诱导小鼠迟发型超敏反应,小鼠尾静脉注射胶体碳悬液,腹腔注射10%羊红细胞免疫小鼠,观察小儿利咽康颗粒剂对羟甲基纤维素钠致炎诱发白细胞游走的抑制作用,对二硝基氟苯诱导小鼠迟发型超敏反应、对小鼠血中碳粒清除速度、对小鼠血清凝集素的影响。

The assay, which was based on primers and TaqMan-MGB probes selected from highly conserved regions of the hemagglutinin gene of avain influenza virus subtype H5, was optimized in a reaction system and a condition to improve the sensitivity, specificity and accuracy. In addition, colone technology was used to develop a quantitative PCR format with virus copies amount.

针对H5亚型禽流感病毒血凝素基因保守区域设计特异性引物与TaqMan-MGB荧光探针,筛选并优化荧光定量RT-PCR反应体系与反应条件,用以提高方法的特异性、敏感性与准确性;并通过体外克隆技术建立病毒基因拷贝数进行定量分析。

Here we report a 22year-old man who suffered from recurrent fever accompanied by chest and abdominal pain for more than 10 years. The attack frequency was about once per 23 weeks. Although he consulted many clinics and even received appendectomy at the age of 15, no definite diagnosis was given. During the admission, many laboratory examinations failed to show any abnormality except mild leukocytosis and elevated C-reaction protein. Image studies including chest X ray and abdominal CT scan showed negative result but, interestingly, Gallium-67 scan showed a hot spot in right lower chest and right lower abdomen. After prophylaxis with colchicines 1.0 mg per day, he has enjoyed more than 2 years without the above symptoms.

本篇报告发表一位22岁的男性,十余年来每隔二三周即发生周期性的发烧,浆膜炎;虽然他遍访数家医院,并且在十五岁时被诊断为阑尾炎,并接受开刀治疗,还是没有得到正确的诊断与治疗,此次住院当中,除了稍微的白血球与C-反应蛋白偏高以外,很多实验室检查皆正常,影像学检查包括胸部X光,腹部电脑断层均正常,但有趣的是Gallium-67扫描发现在右胸及右下腹出现强烈反应,经诊断为地中海热后给予秋水仙素预防性治疗,病人已经超过二年没有复发。

Under the conditions of 456 W power output and l0min irradiation time, the quantities of reactants are malonic acid 13.Og and vanillin lOg, in pyridine solvent (1 6m1), the yield of reaction is 80%.

本实验在额定功率为456W,辐射时间为10min下,反应物投料量分别为丙二酸13.6g,香兰素10g在16ml溶剂中进行反应。

Halogen, proton acids, sub-halogen acid are electrophilic reagents, olefin addition reaction is the electrophilic addition reaction .

卤素、质子酸,次卤酸等都是亲电试剂,烯烃的加成反应是亲电加成反应。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

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There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。