反应素

By using fluorescence spectrophotometry method,a systematic study was conducted to determine the effect of cadmium and lead ion stress on reactive oxygen species in creeping bent Agrotis stolonifera leaf tissue.

通过2种重金属离子和抗霉素A处理匍匐翦股颖Agrotis stolonifera幼苗叶片，利用2，'7'-二氯荧光黄双乙酸酯溶液对重金属胁迫和抗霉素A处理后叶片中产生的过氧化氢(H2O2)反应形成具荧光物质DCF的特性，用荧光光度分析法分析了叶片中H2O2产生与积累规律。

66 patients had been done coronary angiograph and divided randomizely into drug-eluting stents implantment group(30 cases of Cypher stent implantment) and common stents implantment group(36 cases of common stent implantment).

目的：比较药物洗脱支架与普通支架植入前后冠心病患者血超敏C-反应蛋白、白细胞介素-6（IL-6）、P选择素、可溶性细胞间粘附分子（sICAM-1）的变化，探讨其意义。

A one-pot chemoselective reduction and lactonization of(4S,5R)-hemiesters was carried out to afford high-yielding(3aS,6aR)-lactone,a key chiral building block for the biotin synthesis. One-step introduction of carboxybutyl chain onto(3aS,6aR)-thiolactone was reached upon treatment of zinc reagent 25 in the presence of nanopalladium via an improved Fukuyama coupling under mild reaction conditions.

在侧链引入过程中，采用纳米钯LDH-Pd~0为催化剂以改进的Fukuyama偶联反应一步高效地引入-生物素五碳羧酸侧链，紧接着创造性地以离子氢化的方式建立了-生物素的第三个手性中心。

During our strategy, two linear amplification approaches are employed, ligase detection reaction and an isothermal amplification (cooperation by DNA polymerase and nicking enzyme).

连接产物由于在DNA聚合酶和限制性内切酶的共同作用下，发生等温扩增反应，扩增出大量的血红素的适配子，适配子与血红素结合，形成的复合物催化鲁米诺和双氧水的化学反应。

Levels of expression significantly increased after day 4 posttransplantation and persisted through the whole course of rejection, but levels of expression were obviously lower in the peripherial blood than those in portal vein blood. In contrast, the control group showed no expression of mRNA for perforin and granzyme B in portal vein and peripherial blood.

结果 急性排斥肝移植组术后3天门静脉和外周血中均有穿孔素和颗粒酶B基因mRNA表达，4天后表达明显增强，并贯穿整个排斥反应的全过程，但外周血表达的强度明显较门静脉血为弱，同基因对照组术后14天内无穿孔素和颗粒酶B基因表达。

Results The rejection group began to show the mRNA of perforin and granzyme B up-regulation on day 3 posttransplantation, when no histologic evidence of rejection was found. Levels of expression significantly increased after day 4 posttransplantation and persisted through the whole course of rejection, but levels of expression were obviously lower in the peripherial blood than those in portal vein blood. In contrast, the control group showed no expression of mRNA for perforin and granzyme B in portal vein and peripherial blood.

结果 急性排斥肝移植组术后3天门静脉和外周血中均有穿孔素和颗粒酶B基因mRNA表达，4天后表达明显增强，并贯穿整个排斥反应的全过程，但外周血表达的强度明显较门静脉血为弱，同基因对照组术后14天内无穿孔素和颗粒酶B基因表达。

In order to understand their mechanisms of blood-lipid regulation, We used the amphotercin B cell model and RT-PCR method to examine the effects of the serum-drugs of SM and FC extracts and their effective monomers, including danshensu, protocatechualdehyde, ursolic acid, quercetin and rutin, on cholesterol biosynthesis in CHO cells and the expressions of CYP7AmRNA and DGATmRNA in BRL cell line.

采用两性霉素B细胞模型和逆转录-聚合酶链反应方法，分别观察丹参和山楂各调脂有效部位以及部分单体(丹参素钠、原儿茶醛、熊果酸、槲皮素和芦丁)对内源性胆固醇合成、CYP7AmRNA和DGATmRNA表达的影响。

The expression changes of ICAM-1 and p65 mRNA level were detected by reverse transcription-polymerase chain reaction and the expression of p65 protein in AR42J cell lines were detected with Stept Avidin-Biotin complex respectively, and the changes of amylase in culture medium with chromatometry.

逆转录－多聚酶链反应半定量法观察细胞间黏附分子(ICAM-1)和p65亚单位mRNA表达的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达；人工碘比色法观察培养液上清中淀粉酶的活性改变。

The expression changes of TNF-α and p65 mRNA expression were detected by reverse transcription-polymerase chain reaction, the TNF-α level in the supernatant was determined by ELISA, and the expression of p65 in AR42J cell lines was detected by Stept Avidin-Biotio Complex.

逆转录－多聚酶链反应半定量法观察肿瘤坏死因子/p65亚单位mRNA表达的变化；ELISA法检测TNF-α在细胞上清中质量浓度的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达。

Bombyx mori silk fibroin film was treated by plasma processes with SO 2 gas to introduce functional groups on the surface of film. Meanwhile, functional groups were also introduced by the reaction of 1,3 propane sultone with amine sites on the surface of silk fibroin film which were produced by NH 3 gas plasma.

从蚕丝中提取的丝素蛋白制备成薄膜，首次采用二氧化硫等离子体处理在材料表面引入磺酸基团；同时丝素蛋白膜用氨气等离子体处理后在表面接入氨基，利用 1，3 丙磺酸内酯与氨基的反应在材料表面接枝磺酸基团。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。