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The PTZ plasmid vector was changed to plasmid vector pSP72, which contains both T7 and Sp6 promotors for transcription of antisense (T7) and sense (Sp6) RNA probe in vitro. The results revealed the label rate of cRNA probe is high, the hybrids between RNA-RNA are stable, so the background of hybridization is satisfactory.

本实验将质粒载体PTZ转换为带有T7和Sp6两个启动基因的pSP72载体,故可在体外转录cRNA时同时得到意义(Sp6为启动基因)和反意义(T7为启动基因)探针,实验结果表明,cRNA探针标记率高,杂合物稳定,故所获杂交反应背景较为满意。

Many other reactions catalyzed by phosphines have also been studied.

进而用2,3-联烯酸叔丁酯来研究[3+2]环加成反应合成螺环化合物,区域选择性有很大提高,产率也很好。

Using t-tset to analyze significance of difference. Results: 1. H101 could cause cytopathic effects in U251 cells from 24h and shows greater oncolysis effects when much more H101mRNA expression by enhanced virus tite or prolonged time.

结果: 1。 H101病毒在24小时就可以对体外培养的胶质母细胞瘤U251产生抑制作用,加入的病毒滴度越大抑制率越高,并且随着病毒作用时间的延长,细胞病变反应越发明显,H101mRNA的表达呈上升趋势。2。

Although many methods on the oxidation of olefines to their derivatives were reported,there are many problems concerning low yield,catalyst lost,harsh condition,and serious pollution.

在现有的合成方法中,尚存在产率不高,催化剂流失,试剂昂贵,反应条件苛刻,环境污染严重等问题。

248Nm zero-order nulled chirped silica phase masks are fabricated by insertion a cylindrical lens to 325nm He-Cd laser holographic system to form the chirped grating patterns on photoresist and then transfer the patterns to silica substrates by reactive ion etching.

采用在325nm He-Cd激光双光路全息干涉系统的一臂上插入柱面镜的方法和反应离子刻蚀技术研制了周期渐变的零级抑制石英相位掩模版,并用该掩模版在B/Ge双掺光纤上研制出中心波长1551nm,带宽11.4nm,反射率约60%的宽带Chirp光纤光栅。

The result obtained was very promising. Meanwhile, [3+2]-cycloaddition of aziridines with nitriles was discovered by using Sc3 as catalyst.

与此同时,我们还发现了Sc3可以很好地催化腈与2-芳基氮杂环丙烷的[3+2]环加成反应,得到了二氢咪唑产物,产率中等到优秀。

A variety of aldehydes were converted into acylals in good yields with anhydride in the presence of a catalytic amount of zinc bromide under solvent-free conditions.

以醛和酸酐为原料,溴化锌为催化剂,无溶剂条件下高产率地合成了偕二酸酯(其中醛与苯甲酸酐的反应是首次报道)。

The result shows that reaction time for tertiary amine formation is only 7min, and the yield is increased by 25%.

结果表明,生成叔胺的反应时间缩短为7min,产率增加了25%。

The reactive interface has been studied under high magnification microscope, and it's verified that there were reaction induced mixing phenomena in RIM polyurethane system.

本文首先是在较高倍率的显微镜下观察了两相的反应界面,进一步证实在RIM聚氨酯体系中存在着化学反应诱导混合现象。

All the experimental operations were carried out under the conditions of water-free and oxygen-free in nitrogen stream (99.99%).

实验所有操作是在氮气流保护下,无水无氧操作,所用溶剂是经过除水、除氧回流后方可使用,反应原料无水氯化稀土是按文献方法合成,产品产率较高。

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