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After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定:采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒,将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合,靶细胞被裂解后,释放的乳酸脱氢酶与底物反应显色,酶标仪测定吸光度,吸光度与裂解程度成正比,按照公式计算百分裂解率。

Currently the dosage forms of the treating drug are ordinary oral tablet, suppository, clyster and so on.However, these dosage forms have shortages, such as used inconvenience, the efficacy is not ideal, relapse rate is high and number of adverse reactions is more.

目前其治疗药物主要是采用普通的口服片剂、栓剂、灌肠剂等剂型,但这些剂型存在使用不便、疗效不理想、复发率高和不良反应多等缺点。

The paper includes:The author calculate the structures inner force and displacement of the free end , analyze the influence of the height and the value of pretress of cable by making use of ANSYS 9.0 soft, pose the concept of critical prestress,conclude the function relation between displacement of the free end, initial strain and the height of cable ,analyze the dynamic properity and point out that Newmark-β method should be adopted when we analyze the dynamic properity of the cable-stayed spatial structure with direct integral test to guarantee the contribution of high mode,finally conclude the functional relation among coefficient of earthquake inner force, initial strain and the height of cable, wish to get some helpful conclusion applied to cable-stayed spatial truss design.

本文系统介绍了斜拉空间桁架竖向地震反应的分析理论与方法、详细分析了斜拉空间桁架结构在静力和竖向地震作用下的受力性能,主要内容包括:利用通用有限元软件ANSYS9.0计算了斜拉空间桁架在静力和竖向地震作用下内力及自由端位移分布规律并得出地震内力系数与节间位置、预应力以及斜拉索高度之间的函数关系;详细分析了斜拉索中施加的预应力以及斜拉索高度的变化对于该种结构内力及自由端位移的影响并提出了斜拉空间桁架的临界预应变概念,进而得到了自由端位移与初始预应变以及斜拉索高度的函数关系,即:△=-(40h+2180)ε+9.8;分析了该种结构的动力性能,并指出:在时域内采用直接积分法计算该种结构的动力响应时,应该考虑采用不引起振幅率减小的Newmark-β法,以保证高阶模态响应的贡献仍然保留在响应中而不致于被忽略。

Methods The different density of CO2 was gained by chemistry reaction and the trap killing-rate was tested by comether experimentation.

方法不同浓度CO2运用化学反应获取,通过诱蚊试验测定诱杀率反应诱杀效果。

Results: The HD50 of PNS was higher than 4mg·ml-1 , and PNS significantly increased concanavalin A and lipopolysaccharide-stimulated splenocyte proliferation in a concentration-dependent manner in vitro.

结果: PNS的半数溶血率HD50大于4mg·ml-1;体外显著促进了刀豆蛋白和脂多糖诱导的小鼠脾淋巴细胞增殖反应。

What's more, we measure the inhibition rate of calcium carbonate and conglutination of the polymaths.

以丙烯酸、顺丁烯二酸酐和丙烯酰胺为原料,过硫酸钾为引发剂,在不同的条件下进行自由基聚合反应,生成不同分子量的共聚物,并测定了这些共聚物的粘度和对碳酸钙的阻垢率。

The test results showed that the amount of this kind of function material to conjugate proteins were larger than 1.5ig/mm2and its one time adsorption rate was larger than 80% and can satisfy the requirements for solid-phase radioimmunoassay. Test results showed that this polymer-NHS-ester can connect with proteins through covalent bounds under mild conditions This technical method will supply a type of new material for solid-phase radioimmunoassay.

实验证实,这类聚合物NHS酯可在温和条件下与蛋白反应,使蛋白以共价键结合方式固定在聚合物上,这种功能材料对蛋白的结合量大于1.5μ/mm~2,对蛋白的一次吸附率大于80%,能满足固相放免分析的要求,将为固相放免分析提供一种新材料。

Results The postoperative reaction was mild and all the transplanted conjunctiva was alive.

结果 术后反应轻,移植片均存活,降低了复发率。

The leaching process can be explained by the model of contractibility and unreacted core, that is the leaching rate conforms to the leaching equation 1-(1-α)(superscript 1/3)~kt.

浸出过程可用收缩未反应核模型来解释,即浸出率与动力学方程1-(1-α)(上标 1/3)~kt相吻合。

And the relationship between the weight average molecular weight of the copolymer and its scale inhibiting was analyzed.

考察了反应条件对共聚物产率和重均分子量的影响,并研究了重均分子量与其阻垢性能的关系。

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

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本文综合考虑了几种电弧重燃和熄灭理论。