反应物

The reaction mechanism of H2O2 dismutation catalyzed by a non-heme tetraaza annulene complex has been investigated by density functional theory using the B3LYP functional.

采用密度泛函理论B3LYP方法计算了一种非血红素四氮杂轮烯配合物催化H2O2歧化的反应机理。

Superoxide dismutase (EC 1.15.1.1;SOD) is a metal-containing enzyme that widely exist in organisms. It catalyzes the dismutation of superoxide free radical and produces hydrogen peroxide and oxygen.It plays an important role in protecting the plasmalemma from the reactive oxygen species.A recombinant plasmid pPICZA-sodM18 was constructed and after its linearization was transferred into chromosome of Pichia pastoris GS115 by electroporation.

超氧化物歧化酶(Superoxide dismutase；EC 1.15.1.1；SOD)是广泛存在于生物体内的金属酶，是机体内唯一以超氧阴离子自由基(O_2~-)为底物的酶，它能专一地催化O_2~-的歧化反应，生成过氧化氢和氧气，能有效的抵御氧自由基和其他氧化物自由基对细胞质膜的毒性，对机体防御氧化损伤起着十分重要的作用。

When termination occurs partly by disproportionation, the prepolymer chains in the product carry branches as well as cross-links.

当终止反应一部分是由歧化所起时，则在产物中预聚物链不但带有交联也带有支链。

Detection of 98% of DMD/BMD gene deletions by polymerase chain reaction.

应用14对引物多重聚合酶链反应作假肥大型肌营养不良症的产前基因诊断。

Cucumber mosaic virus isolation CP310 was isolated from Capsicum annuum.By detecting 14 kinds of host plants,Nicotiana glutiana and Nicotiana clevelandii with CP310 showed dead symptoms and Lycopersicon esculentum and Nicotiana tobacum with CP310 showed dwarfish symptoms.

在辣椒上分离到一株黄瓜花叶病毒分离物CP310，对其进行14种寄主植物生物学反应测定，发现CP310在心叶烟、克里夫兰烟等植物上表现致死症状，在番茄、烟草表现为植株明显矮化。

Results: The evaluation at the middle of period of treatment: the variations of pulmonary rales, perpheral bloody white blood cells and the X-ray examination an chest of the two groups' patients wren similar, but the symptom of fever and character of respiratory tract's secretion of the patients in experiment group took turns for better obviously than the control group's, the rate of elimination for ineffective on 3 day of the experiment group was obviously lower than the matched control. The evaluation at the end of period of treatment: the total effectualness and elimination of bacterium of the experiment group were notably higher than the matched control, There were not any obvious untoward reaction.

结果 疗程中期，两组肺部啰音、外周血白细胞及胸部X线检查变化相近，试验组发热、呼吸道分泌物性状较对照组明显减轻，3d无效淘汰率低于对照组；疗程结束，试验组有效率和细菌消除率均高于对照组，且未见明显不良反应。

The results indicate that the complex can decrease energy barrier of the titled reaction and it may be a potential candidate for catalase mimic.

这表明标题配合物可有效地降低标题反应的能垒，有可能作为一种潜在的过氧化氢仿酶。

Objective To identify gene mutation of a epidermolysis bullosa pruriginosa family. Methods Polymerase chain reaction, DNA squencing, multiplex PCR using allele-specific oligonucleotide primers.

采用聚合酶链反应，DNA直接测序以及等位基因特异引物的多重PCR方法，对一个痒疹样大疱性表皮松解症家系40人（其中患者23例）进行基因突变情况检测。

Erythrosin e system and ethyl eosin system can be applied to the determination of chlorpromazine hydrochloride with conventional spectrophotometry, and eosin Y system can be used by color fading spectrophotometry.

在NaAc HCl缓冲介质中，盐酸氯丙嗪可与曙红Y、赤藓红、乙基署红等卤代荧光素染料反应，形成离子缔合物，溶液颜色发生明显改变，可用光度法测定。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。