反应物

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

The substrate coupling reaction system catalyzed by recombinanted CAR wasconstructed. It can be used for asymmetric reduction of 2'-chloroacetophenone and coenzyme regeneration with 2-propanol or 2-butanol as the cosubstrate.

5应用重组CAR不对称催化还原2'-氯-苯乙酮，通过加入异丙醇或仲丁醇作为辅助底物，建立了底物耦联反应体系实现辅酶NADH原位再生的反应体系。

In the asymmetric hydrogenation of ethyl pyruvate catalyzed by 0 5% Rh/PVP TiO 2, the chiral modifier cinchonidine not only induces the enantioselectivity, but also accelerates the reaction rate greatly.

研究了辛可尼定作手性修饰剂修饰的负载型纳米铑簇合物催化剂(0 5 %Rh/PVP TiO2 )催化丙酮酸乙酯不对称氢化反应，在该反应中手性修饰剂辛可尼定不仅具有对产物生成的手性诱导作用，而且对反应具有明显加速作用；在优化反应条件后，反应的TOF和对映选择性分别可以达到 5 8 0min-1和

More levels of nano-SiO〓 lead to larger particle size. An increase in polymerization reaction temperature causes the decrease in coagulum and increase in viscosity and particle size, and the nano-SiO〓 has same effects at certain temperature. When the levels of hydrophilic monomer acrylic acid and hydroxylethyl methacrylate in core composite are increased, the particle size of emulsifier-free emulsion decreases, in spite of the presence of nano-SiO〓.

无皂乳液具有单分散性好、表面洁净、成本低廉、不影响产品性能的特点，首次研究了含或不含纳米粒子的含羟基和羧基的丙烯酸无皂乳液聚合，发现含或不含纳米粒子的无皂乳液聚合后的粒子粒径较大，具有单分散性；较多的纳米粒子导致较大的乳液粒径；反应温度升高，凝聚物含量下降，而乳液粘度和乳胶粒粒径增加，在相同的反应温度下，纳米粒子降低了聚合过程中的凝聚物含量，增加了乳液聚合物的粘度和粒径；不论纳米粒子是否存在，当无皂乳液核组成中亲水单体AA和HEMA用量增加时，乳胶粒粒径均减少。

In Chapter 2 of this thesis, the kinetic equation of substrate reaction in presence of denaturant has been derived. A re-examination of the effect of high concentrations of guanidine hydrochloride on the inactivation of papain, taking into consideration the effect of high concentrations of guanidine hydrochloride on the Michaelis constant, showed that, for papain, the substrate gives no protection on its inactivation.

我们在第二章中推导了变性剂存在下的底物反应动力学方程，并在考虑了高浓度盐酸胍对酶催化反应米氏常数的影响的前提下，根据推导的动力学方程重新分析了盐酸胍存在下木瓜蛋白酶的失活动力学，结果表明底物对木瓜蛋白酶的失活没有保护作用。

Furthermore, two kinds of oligonucleotides with same size and difference sequence were successfully separated.

建立了分析2个丫SNP的单碱基引物延伸反应，掌握了单碱基引物延伸反应的规律。

A large number of bond-type complexes, as long as the hydrogen of the molecules covalently coupled to the molecular approach in an electronegative atom up, that constitutes a hydrogen bond, there are intramolecular hydrogen bonds and intermolecular hydrogen bonds of the points, will affect the formation of complex reaction carried out, such as in solution adding dioxa cyclohexane, or tetrahydrofuran, will reduce the alkylation reaction rate.

氢键型络合物为数众多，只要含氢的分子以共价键方式耦合到分子中一个电负性原子上去，即构成氢键，有分子内氢键和分子间氢键之分，络合物的形成将会影响反应的进行，如在溶液中加入二氧杂环己烷或四氢呋喃，都会降低烷基化反应速率。

Additionally, the activities of deltoid, trapeizus and erector spinae were higher in asymmetrical conditions as compared with in symmetrical conditions.

此外，三角肌、斜方肌及背脊肌的反应量在非对称持物姿势下明显高於对称持物姿势下的反应量。

It was shown that the absorption peaks of amine and amide groups of gelatin were shifted in HDPE/PE-g-MAH/Gelatin blends because of the reaction of PE-g-MAH with gelatin. The addition of PE-g-MAH made the increase of HDPE glass transition temperature. The compatibilizing effect of PE-g-MAH was due to the crystal compatibility produced by forming the co-crystals of HDPE, PE-g-MAH and PE-g-MAH-g-gelatin. The blends of EAA and gelatin were prepared by melting process and the compatibility of the blends was also studied. The FTIR results showed that absorption peaks of amine and amide groups of gelatin were shifted in EAA/Gelatin blends.

通过红外光谱分析、HAAKE流变分析、抽提等实验证明：共混物中明胶的氨基、酰胺基团吸收峰峰位发生了明显的变化；在熔融共混过程中，随着温度的提高，平衡扭矩增大；明胶与PE-g-MAH发生了反应形成PE-g-MAH-g-明胶接枝共聚物，而且随着PE-g-MAH用量的增加，形成接枝共聚物的量也提高；SEM的实验结果表明，在明胶的表面确有接枝物形成；通过DMA测试共混物的玻璃化转变温度，发现两组分的玻璃化转变温度差值减小，二者的相容性提高，实现了共混体系的自增容。

Novozym 435 was used for the synthesis of vitamin A plamitate. The influences of solvent, the molar ratio of substrates, the reaction temperature and time, and the water concentration were optimized and the best result was obtained by interesterification from 0.200 g vitamin A acetate and 0.468 g palmitic acid, at 30℃, in 10 mL hexane, containing 0% of water, with 10% of lipase(mass ratio, immobilized lipase to vitamin A plamitate). In these conditions, 75% of vitamin A acetate was converted into vitamin A plamitate. The immobilized lipase was reused about 6 batches.

对催化合成维生素A棕榈酸酯反应的脂肪酶和反应介质比较，同时对影响合成维生素A棕榈酸酯反应的因素（温度、初始水含量、底物摩尔比、反应时间和酶量等）进行了探讨，优化了反应条件：在10 mL不含水分的正己烷中，0.200 g 维生素A醋酸酯和0.468 g棕榈酸在酶量为10％（指固定化酶与维生素A醋酸酯的质量比）的固定化脂肪酶催化下，在 30℃、190 r/min下反应6 h，转化率可以达到75%，固定化酶可连续使用6次以上。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。