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反应抗体

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Results indicate that the serum titers of the two kinds of antibodies all reached 3.2×10^5, however, the antibody produced by the immunogen prepared via glutaraldehyde showed higher affinity. The CTC half inhibition concentration (IC50) was 96.2μgmL^(-1) the detection limit was 6.67ngmL^(-1), and the cross-reaction rate between tetracycline and CTC was 77%. The research layed a good foundation for the detection of the antibiotics in livestock products.

结果表明,2种血清的抗体效价最高均达3.2×10^5%,而戊二醛法制备的抗原产生的抗体具有更高的亲和力,抑制率为50%时的金霉素的质量浓度(IC50)为96.2μmL^(-1),金霉素的最低检测限为6.67ngmL^(-1),金霉素和四环素的交叉反应率为77%研究结果为畜产品中四环素类抗生素残留的检测奠定了较好的基础。

For developing an immunoassay for multiple residues of pyrethroid pesticides, the hapten N-2- carbanmoyl-(3"-phenoxyphenyl) methyl 3-(2"-Cldoro-3",3",3"-trifluoropropenyl)-2, 2-dimethylcyclopropane- carboxylate was synthesized from cyanohydrin and 3-(2"-Chloro-3",3",3- trifluoropropenyl)-2, 2-dimethylcyclopropanecarboxylic acid via hydrolysis and esterification. The synthesis of the hapten was foundation of preparaing anti- cyhalothrin polyclonal antibodies.

为了制备对单一拟除虫菊酯高特异性的抗体,以菊酸和氰醇为起始原料,通过水解、酯化反应合成了半抗原N-2-氨甲酰-(3-苯氧基苄基)甲基-3-(2-氯-3,3,3-三氟丙烯基)-2,2-二甲基环丙烷羧酸酯,为进一步制备抗三氟氰菊酯的特异性抗体奠定基础。

The four most common phenotypes are A,B,AB,and O,referring to the type of antigen is presentor absenton the surface of a client's red blood cells,Routine testing usually involves only the Rh antigen.if an Rh-negative clinet receives Rh-positive blood,he or she will develop Rh antibodies,and future Rh-positive transfusions may cause a transfusion reaction .in pregnancy,antibodies from an Rh-negative mother may hemolyze fetal(erythroblastosis fetalis,or hemolytic disease of the newborn).

四种最普遍的表现型是A、B、AB、阿,这类型的抗原或表面上的一个客户的红血球、常规测试通常涉及到只有Rhantigen.if准备一Rh-positive接受血液,跟他或她将发展Rh抗体和未来的Rh-positive输血可能引起输血反应,在怀孕期间的母亲可能会产生抗体hemolyze北京(erythroblastosis fetalis或胎儿的新生儿疾病溶血试验确定具体)。

Two cases with hemoglobinuria showed low serum haploglobin and indicated intravascular hemolysis. One of them was proved to be a delayed hemolytic transfusion reaction due to anti-Jk.

输血后发生血红素尿的2例当中(血清haptoglobin下降)l例为Jk抗体引起的迟缓性溶血性输血反应;而另1例并没找到不规则抗体。

Results: The natural infection rate of chidren and women of child-bearing age is respectively 5.19%and 75.13%.There is no bad reaction after inoculating the nettle rash vaccine.All Children group antibody transfer to positive, while women of child-bearing age has only one negative, and GMT increase, it is a positive antibody level to propose long notably after vaccine inoculation.

结果儿童和育龄妇女的自然感染率分别为5.19%和75.13%,接种风疹疫苗后无不良反应,儿童组抗体全部转为阳性,育龄妇女仅1例为阴性,几何平均滴度增高,接种疫苗后显著提升抗体水平。

"One of the difficulties — still — is in making the clinical decision of whether a child has type 1 or type 2 diabetes," Dr. Bennett explained. There are several characteristics that differentiate the two. Dr. Bennett reported that among the Pima, 92% of children with type 2 diabetes also had a family history of type 2 disease, most often in the mother. Other features include obesity (body mass index of 30 kg/m2 or greater),(68%), being asymptomatic (78%), and ketoacidosis (about 10%). These children and adolescents are not insulin-dependent and may stay that way for years, he said. They are also glutamic acid decarboxylase antibody– and islet cell antibody–negative.

Bennett博士解释道,在临床上决定孩童是第一型或是第二型糖尿病是有困难的,两者之间是有不同的特点,Bennett博士提到,在印地安人身上,92%的孩童发生第二型糖尿病是有家族史的,且与母亲有关,其他特徵还包括肥胖(身体质量指数大於等於30 kg/m2)占68%、无症状占78%、酮酸血症约占10%;Bennett博士指出,这些孩童与青少年并非胰岛素依赖型且持续好几年,他们在麸氨酸脱羧酶抗体与胰岛细胞抗体是呈阴性反应。

In order to establish a sensitive immuno-PCR assay for detecting serai anti-P53antibodies, investigate the relation of anti-P53 antibodies in patients with breast carcinoma to P53 protein expressions in tissue and clinical significances and afford a practical tool in diagnosis of breast carcinoma clinically. SaHIgG was coupled to PNCa DNA by chemical cross-linker PEI and SaHIgG-DNA probe was constructed.

本研究建立了血清抗P53蛋白抗体免疫聚合酶链反应(Immuno-polymerase chain reaction,免疫PCR)检测方法,探讨了乳腺癌患者血清中抗P53蛋白抗体与组织中P53蛋白表达之间的关系及临床意义,旨在为临床乳腺癌P53蛋白检测提供实用的替代工具。

When adult male rabbit were immunized with rSMP-B, their spermatogenesis was blocked at spermatid stage. It suggested that rSMP-B might play an important role in spermatogenesis and spermiogenesis.

斑点杂交法表明该抗体与人及大鼠睾丸细胞质中的一种特异性蛋白发生交叉反应,提示rSMP-B具有种属同源性,其抗体有较强的抗育作用。

The effect of thymus environment on T cell development and tolerance.

在所挑选的这些抗体中,并不是所有对细胞系阳性的抗体对正常的细胞也呈阳性反应,说明培养的细胞可能表达了一些正常细胞不表达的抗原。

Sera from patients with other parasitic diseases and healthy individuals were used as control. RESULTS: The anti Trichinella antibodies were detected in 15 infected mice as early as 2 weeks after infection,and in 86 5% of 467 patients with trichinosis.

结果:15只小鼠感染旋毛虫后2wk即可检出抗体,467例旋毛虫病患者的抗体阳性率为86.5%,而丝虫病、包虫病患者各45例和健康人血清56份均为阴性。48份并殖吸虫病患者的交叉反应率为16.67%。

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